3 cell membrane samples: A, B, and C were fluorescently labeled and subsequently photobleached. The recovery of fluorescence is given on the FRAP curve: (see image attached)Rank the 3 cell membranes in terms of DECREASING fluidity and explain your ranking based on their FRAP curves, most probable appearance, and composition of the membranes.Draw the expected image of the surface of the 3 membranes BEFORE photobleaching and AFTER fluorescence recovery as viewed under a fluorescence microscope. 100AВtime% fluorescence

Fluorescence recovery after Photobleaching ( FRAP )-- Fluorescence recovery after Photobleaching is…

Answered: 3 cell membrane samples: A, B, and C…

3 cell membrane samples: A, B, and C were fluorescently labeled and subsequently photobleached. The recovery of fluorescence is given on the FRAP curve: (see image attached)

Essentials of Pharmacology for Health Professions

7th Edition

ISBN:9781305441620

Author:WOODROW

Publisher:WOODROW

Chapter5: Abbreviations And Systems Of Measurement

Section: Chapter Questions

Problem 5CRQ

See similar textbooks

Related questions

Q: Channel proteins used for the bulk passage of water molecules across the cell membrane?

Q: This lab examines the relationship between the absorbance of light by a solution at 595 nm and the…

A: Bovine serum albumin or BSA is a serum albumin protein derived from cows. It is often used as a…

Q: Red blood cells look intact and normal in the 0.9% solution. In the 1.5% solution cells are present…

A: When RBCs are placed in 0.9% saline solution, there is no net movement of water in any direction.…

Q: Asnwer the given question a and b, DO NOT JUST COPY PASTE IMAGES FROM GOOGLE

A: FRAP is the Fluorescence Recovery After Photobleaching. It is the technique that used the…

Q: Gold particle size range nanometers show the highest cell uptake. Larger particles, for example,…

A: Gold particles range from around 50nm show highest cell uptake. Larger particles, for example,…

Q: applicability of a dialyzing membrane (DM) as a model for the cell membrane enclosing an aqueous…

A: Diffusion the transport of molecules from their high concentration towards their low concentration.…

Q: Relative fluorescence intensity (A.U.) 1.0 0.8 0.6- 0.4 0.2 dithionite 0.0+ 0 100 Liposomes…

A: Answer :- The erythrocyte extract given here contains enzymes/proteins known as phospholipid…

Q: 5) Using FRAP (Fluorescence Recovery After Photobleaching), you can measure the diffusion rate of…

A: FRAP (fluorescence recovery after photobleaching) is a method to determine the diffusion kinetics…

Q: a. Rank the three cell membranes in terms of decreasing fluidity. Provide an explanation of your…

A: The plasma membrane is the outmost layer that holds the inner constituents of the cell and prevents…

Q: Soap molecules have a polar head and a non polar tail. Briefly explain whether your experimental…

A: Soap is prepared by combining a fatty acid with a base. It breaks the carboxyl bonds to form soap…

Q: A large chaperone protein complex GroEL is approximately 16 nm in diameter. When it is dissolved in…

A: Particles present in cells show rapid movement(back and forth) and we call it Brownian motion. The…

Q: ) The diameter of the Kupffer cell in the image is 9.1 cm. Assuming it is spherical, calculate the…

A: Kupffer cells are specialised cells that are found in the liver's sinusoids' lumen and are adherent…

Q: Research Center received a protein sample in a liquid form from her colleague recently. However, the…

Q: Consider this red blood cell (red circle) in the solution. The blue dots cannot pass through the…

A: The ability of a solution that enables the transport of water in or out of the cell by the process…

Q: Why both acidic and basic dye can be used to stain cytoplasm? Why the negative charged Eosin can…

A: Acidophilic Dyes: These are the acidic dyes which react with basic or cationic components in the…

Q: 2. How does SDS-PAGE separate proteins and peptides from each other? Explain

A: Note-''Since you have asked multiple questions, we will solve the First two questions for you. If…

Q: Troubleshooting gels. What effect will be observed on the

A: Polyacrylamide gel electrophoresis is used for the separation of protein molecules on the basis of…

Q: the length of Cell X using the ruler in Microsoft Word. Assuming the actual length to be 3 um,…

A: Introduction Bacteria are single-celled microorganisms with a simple structure. Bacteria can be…

Q: Two wavelengths of light strike a living cell, one has a wavelength of 12 nm, and one has a…

A: A wavelength can be defined as one cycle of wave and it's measured as the distance between two…

Q: Diffusion Spot Diameter 1 Diffusion Spot Diffusion Spot Diameter 3 Time Diameter 6 (min) Crystals…

A: Diffusion is the process by which molecules move from a region of higher concentration to a region…

Q: You are interested in the mobility of two different unknown integral plasma membrane…

A: Here after the cDNA transformation from the mRNA with the help of reverse transcriptase the protein…

Q: Three cell membrane samples: M, C, and S were fluorescently labeled and were subsequently…

A: Introduction: The cell membrane, also known as the plasma membrane, is a semi-permeable membrane…

Q: a. You selectively label phospholipids with a fluorescent dye and perform the FRAP assay. You detect…

A: FRAP is a method to determine the diffusion or kinetics of the biomolecule through tissue or cells.…

Q: hypotheses about the fluidity and mosaicity of the cell membrane. Write the hypotheses in "IF-THEN"…

A: Cell membrane of plasma membrane is the outer layer of the cell. It performs several function first…

Q: 4. In an experiment to determine the water potential of fresh beetroot tissue, a student cut 12…

A: A. Beet root contains a special pigment betalains in their stem tubers. This pigment help to know…

Q: Diameter of a typical cancer cell Range of alpha particle Range of beta particle 100 um 50-70 um 700…

A: The cancer is a condition of uncontrolled cell division and growth. In Cancer cell certain genetic…

Q: 1. In Gel filtration chromatography, when will you stop collecting eluents if sample is not colored?…

A: As you have posted more than one question, we will solve first question for you. I request you to…

Q: Diameter of a typical cancer cell Range of alpha particle Range of beta particle 100 um 50-70 um 700…

A: Radiotherapy or radiation therapy is the use of high intensity radiation to kill or suppress the…

Q: Please label this red onion cell image with a drop of water under the microscope. Include all…

A: The movement of nutrients, water, and waste in and out of the cell is required for the living…

Q: Membrane fluidity [ Choose ] depends on lipid composition, temperature, pH, and ionic strength…

A: Lets see that the statement given is authentic or false. There are 6 question in a row with…

Q: 5- a sample of a crude protein extract is obtained from a prostate cancer tissue read 0.98 at 280 nm…

A: According to Christian and Warburg method, if the sample have the *presence of nucleic acid also,…

Q: Our current experimental procedure works because Con A adheres strongly to the nitrocellulose…

A: The correct option is C A membrane-binding protein.

Q: Please label this microscope image of sheep's blood in a hypotonic solution with as much details as…

A: Tonicity of a solution is the measure of the relative difference of the solute concentration or the…

Q: 3 cell membrane samples: A, B, and C were fluorescently labeled and subsequently photobleached. The…

A: FRAP or fluorescence recovery after photobleaching analytical method that helps to determine the…

Q: \ctivity D: Osmosis in an Onion Cell this activity you will observe what happens to onion cells when…

A: The cells are the structural and functional unit of an organism that perform all the physiological…

Q: an experiment to study the effect of solvent on the integrity of the membrahe of carrot-root cells.…

A: Aim: To study the effect of organic solvent on the integrity of the membrane of carrot root cells.…

Q: The graph below shows the results of a FRAP analyses studying Membrane Protein 1-4 (MP1-MP4). Work…

Q: In a monolayer of cultured epithelial cells, the cells may or may not have formed tight junctions.…

A: One of the types of cell junctions is the tight junction. Tight junctions are otherwise called…

Q: drug is order 100mg/m2 po BID. How many milliliters will a patient receive if her BSA is 1.80m2 and…

A: The given information told to us is that Order of the drug is 100 milligram per metre square . The…

Q: This was previously rejected due to not including all parts of the question. I've added all parts in…

A: Since you have posted a question with multiple sub-parts, we will solve first three subparts for…

Q: In an effort to develop a novel graft and new intellectual property, you are considering…

A: The process of layer-by-layer (Lbt) deposition for creating thin films. The films are made by…

Q: This lab examines the relationship between the absorbance of light by a solution at 595 nm and the…

A: CBB binds are generally helped to basic amino acids by a combination of hydrophobic interactions and…

Q: Why methylene blue, a basic dye, can be used to stain cytoplasm, a basic structure? Why cytoplasm is…

A: Stains or dyes are the chemical compounds contains the groups that imparts color and their chemical…

Q: A netlike membranous complex of superposed flat saccules with vesicles detaching from the…

A: Endomembrane system: - it is a system through which the cell modifies, packages and transports the…

Q: Educator Materials 3. The average human skin cell measures 30 um in diameter. Calculate this…

A: Skin is composed of three primary layers: the epidermis, the dermis, and the hypodermis. Human skin…

Q: Determine the following for positive staining. (a) dye, (b) charge of the chromophore/colored ion,…

A: A) basic dyes are used to paint the specimen and the background colour is bright B) the coloured…

Q: Hoechst 33342 is a membrane-permeant dye thatfluoresces when it binds to DNA. When a population…

A: The most well-known and most used dye for the visualization of DNA is ethidium bromide. It can…

Q: Three cell membrane samples: M, C, and S were fluorescently labeled and were subsequently…

A: Fluorescent Recovery After Photobleaching (FRAP) is a technique used to test the mobility…

Q: In an experiment to determine the water potential of fresh beetroot tissue, a student cut 12…

A: The spontaneous net movement or diffusion of solvent molecules through a selectively permeable…

Concept explainers

Osmosis

In simple words, osmosis is the slow, gradual movement of a liquid through a membrane. But there are nuances to this process. For instance, there has to be an imbalance in the distribution of solute in the liquid on either side of the membrane, which has to be semipermeable. The liquid in the region with a lower solute concentration moves to the region with a higher concentration, thereby displacing the solution in the process. The objective is to equalize the concentration of molecules on both sides of the membrane; the displacement of liquid can be viewed as a byproduct of this equalization. Since there is no energy required to carry osmosis, it is considered a passive process.

Question

100%

3 cell membrane samples: A, B, and C were fluorescently labeled and subsequently photobleached. The recovery of fluorescence is given on the FRAP curve: (see image attached)

Rank the 3 cell membranes in terms of DECREASING fluidity and explain your ranking based on their FRAP curves, most probable appearance, and composition of the membranes.
Draw the expected image of the surface of the 3 membranes BEFORE photobleaching and AFTER fluorescence recovery as viewed under a fluorescence microscope.

Expert Solution

This question has been solved!

Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.

This is a popular solution!

SEE SOLUTION Check out a sample Q&A here

Step 1

VIEW

Step 2

VIEW

Step 3

VIEW

Trending now

This is a popular solution!

Step by step

Solved in 3 steps

SEE SOLUTION Check out a sample Q&A here

Knowledge Booster

Learn more about

Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.

Similar questions

Solution!
d/e/1FAIpQLSfTle9UfP15_VUqFI-ACEQd1XBykXv5Lr4dEMQbLJ1d6fCupw/viewform Students subjected three samples of five different molecules to gel electrophoresis as shown in Figure 1 A B C DE +2 3 Wells 4 8. Which of the following statements best explains the pattern seen on the * gel with regard to the size and charge of molecules A and B? 1 point molecules A and B are positively charged, and molecule A is smaller than molecule B. molecules A and B are positively charged, and molecule A is larger than molecule B. molecules A and B are negatively charged, and molecule A is smaller than molecule B. molecules A and B are negatively charged, and molecule A is larger than molecule B. Sign out
Please do not give solution in image format thanku
Copy and paste the link below and watch the video on Youtube https://www.youtube.com/watch?v=8RBs0Ghg_48 Answer the following Questions: 1. What are the chemicals and materials used in gel electrophoresis? 2. Draw a schematic diagram of a gel electrophoresis set-up 3. Describe the procedure in doing a gel electrophoresis experiment. Why is there a need for a leveling bubble/leveler? What is the use of the rubber dam? 4. What is the use of ethidium bromide and why must you wear gloves when you handle it? 5. What makes the DNA fragment move towards the positive plate? 6. What is the purpose of glycerol in the sample buffer? 7. What is the use of a DNA ladder? 8. What will happen when you increase the voltage of the set-up? 9. Can gel electrophoresis be used to separate amino acids? If so, how is it done?
Handwritten solution please with detail explanation
Fischer projection of xylitol (sweetening agent in "sugarless gum", candy, and sweet creals.)
Paper chromatography
Relative fluorescence at 603 nm 1.0 0.8 0.6 0.4 0.2 0.0 L 0.00 0.10 0.20 0.30 Time (s) 0.40 0.50 0.60 In this FRET data table, an antibiotic was added, but the type was not recorded. According to the results, could the buffer being used be either negamycin, tetracycline, rifamycin, or cycloheximide? Explain why based on the FRET data.
Milligrams 47.0 milligrams = Multiple Choice 0.47 decigrams 4.7 centigrams 0.047 grams 0.00047 hectograms All of the answer choices are correct.
sicruz@student.f x olfu.instructure.com/courses/137195/quizzes/2350874/take 2022 2023 ents ons ements tions utton HPCT 311 LAB MEDTECH 3-YA-1 X rive Question 14 10% aqueous nitric acid Nitric acid based decalcifying agent best used for staining nuclear and cytoplasmic structures at the same time. O Formol-nitric acid O Phloroglucin-nitric acid O None of the choices. Question 15 Which of the following is a chromic acid based decalcifying agent? Flemming's fluid O Zenker's fluid Von Ebner's fluid O Perenyi's fluid HPCT 311 Midterm Topics - YouT X Question 16 Acts as both fixative and decalcifying agent. POCO X4 PRO 5G Flemming's fluid Quiz: Midterm Practical Quiz L X (1) Facebook X 1 pts 1 pts 11/10/2022 15:40 1 pts
complete solutions please
Fluorescence intensities depends on the molecular structure and its environment. Discuss the factors that can affect the fluorescence intensity and explain how the factors increase or decrease the intensity.