Educator Materials 3. The average human skin cell measures 30 um in diameter. Calculate this diameter in the following units. Show your work. (30um | 1000 - 0.0 0.03m) 6,03mm a. millimeters (mm) b. nanometers (nm) 30,000 ( 30 um x ,000 nm lom - 30,000 um
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- A flat (not spherical) tissue engineered skin (thickness 2.5 mm) is attached to the bottom of a dish and cultured with cell media atop it (which has an oxygen concentration of 0.15 mol/m3). The diffusivity of oxygen in the skin is 3.4 E-9 m2/s, and according to experiments that you’ve performed, you want to make sure that the concentration of oxygen in the device doesn’t fall below 0.06 mol/m3. (Below that concentration harms the cells.) The rate of oxygen consumption is -5.9 E-17 mol/[cell◦s]. What is the maximum cellularity (in cells/mL, three significant digits) that this tissue engineered skin can support?2. Compute for the calibration factor. Show your solution. CF = stage micrometer divisions subtended by om x value of one stage µm division ocular micrometer divisions subtended by stage micrometer (1 su x 10μm) 10 ou CF = CF = 1μm Ocular scale Stage micrometer scale or CF = Computation: (stage units x 10μm) ocular units 60 70 80 90 100 Name of Organism: Organism A Objective Used: HPO Total Magnification: 450x Ocular Micrometer units (violet rods): 10 Length of Organism:Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) 1. What determines the current in your gel? What could cause the current in your gel unit to be lower than expected? Is there anything that could cause it to be higher than expected? 2. Why do you need to wash the gel before staining it? Why use warm water?
- The reults for the macroscopic part: 0.30M glycerin – solution was translucent (could see text behind the test tube) 0.15M NaCl – solution was opaque (could not see text behind the test tube) 0.30M NaCl – solution was opaque (could not see text behind the test tube) 0.15M glucose – solution was translucent (could see text behind the test tube) 0.30M glucose – solution was opaque (could not see text behind the test tube) 0.30M Urea – solution was translucent (could see text behind the test tube) Results for microscopic part: 0.30M glycerin – no cells present 0.15M NaCl – normal sized cells 0.30M NaCl – crenated (shrunken and star-shaped) cells 0.15M glucose – no cells present 0.30M glucose – normal sized cells 0.30M Urea – no cells present Determine the osmolarity (hypoosmotic, isosmotic, or hyperosmotic) and tonicity (hypotonic, isotonic, hypertonic) of the following solutions.In which solutions did the osmolarity NOT match the tonicity? For those solutions, why did the osmolarity…A flat (not spherical) tissue engineered skin (thickness 3.9 mm) is attached to the bottom of a dish and cultured with cell media atop it (which has an oxygen concentration of 0.15 mol/m3). The diffusivity of oxygen in the skin is 3.2 E-9 m?/s, and according to experiments that you've performed, you want to make sure that the concentration of oxygen in the device doesn't fall below 0.06 mol/m3. (Below that concentration harms the cells.) The rate of oxygen consumption is -5 E-17 mol/[cell•s]. What is the maximum cellularity (in cells/mL, three significant digits) that this tissue engineered skin can support?the optical density of your cell culture is 0.92. How much of this culture should you add to 500 mL of LB to obtain a starting optical density of 0.1?
- Put the following steps for running a gel in order. first Remove comb and put gel int v [ Choose ] Load samples into gel Let gel solidify for 15-20 minutes Connect electrodes and turn on power to run gel second Pour heated, liquid agarose into gel cartridge Remove comb and put gel into bottom of electrophoresis box Pour buffer into electrophoresis box to cover gel third fourth Pour heated, liquid agarose in v fifth Load samples into gel sixth Connect electrodes and turn vCopy and paste the link below and watch the video on Youtube https://www.youtube.com/watch?v=8RBs0Ghg_48 Answer the following Questions: 1. What are the chemicals and materials used in gel electrophoresis? 2. Draw a schematic diagram of a gel electrophoresis set-up 3. Describe the procedure in doing a gel electrophoresis experiment. Why is there a need for a leveling bubble/leveler? What is the use of the rubber dam? 4. What is the use of ethidium bromide and why must you wear gloves when you handle it? 5. What makes the DNA fragment move towards the positive plate? 6. What is the purpose of glycerol in the sample buffer? 7. What is the use of a DNA ladder? 8. What will happen when you increase the voltage of the set-up? 9. Can gel electrophoresis be used to separate amino acids? If so, how is it done?I. Identify the approximate amount of the visible band II. Describe the trouble shooting problem from the gel
- Milligrams 47.0 milligrams = Multiple Choice 0.47 decigrams 4.7 centigrams 0.047 grams 0.00047 hectograms All of the answer choices are correct.NMR is considered a low-sensitivity spectroscopic technique. What is the physical basis of NMR's insensitivity?Saved Sort the following characteristics based on whether they apply to light or electron microscopes. 2000x 1,000,000x or more 200 mm (0.2um) Yes No Usually not 0.5 nm Table 3.3 Comparison of Light Microscopes and Electron Microscopes Characteristic Light or Optical Electron (Transmission) Useful magnification 200 mm (0.2µm) 1,000,000x or more Maximum resolution 200 mm (0.2um) 1,000,000x or more Image produced by Light rays Electron beam Image focused by Glass objective lens Electromagnetic objective lenses Image viewed through Glass ocular lens Fluorescent screen Specimen placed on Glass slide Copper mesh Specimen may be alive Yes Specimen requires special stains or treatment Depends on technique Yes Shows natural color Reset