Bacterial Growth Report Only

and the minimum exposure time 1. Determine the minimum inhibitory concentration given the following data: Concentration of ethanol Number of colonies of E. coli on Nutrient Agar plates Exposure time (in minutes) 10 5 15 Distilled water +++ +++ +++ 40% +++ ++ + 70% 95% Minimum inhibitory concentration: Minimum exposure time:

DILUTION COLONY COUNT CFU/mL 1:106 155000   1:107 15500   1:108 1550   1:109 155   Given these values how would I fill in the rest of this serial dilution table? Also, what would be a 1:1 CFU/mL value based on this table?

Apple puree was analyzed for petulin by HPLC-MS-MS after SPE clean-up.  The procedure was 10.0g of puree + 10μl of a 10μg/ml solution of isotopically labeled petulin as internal standard were treated with 10.0ml of pectinase and acetic acid, centrifuged, and filtered.  Four ml of the filtrate was passed through a SPE cartridge.  The petulin was eluted with 2.0ml of ethyl acetate.  The sample was evaporated to dryness and the residue dissolved in 1.0ml of the mobile phase. The analyte signal was 127 and the internal standard 197.  Calculate the concentration of petulin in the sample in μg/g (RRF=1).

Confluence cells added onto 100-wells plate, 2 ml trypsin added onto cells and 6 ml media added to dilute trypsin, 1 ml was used for counting, and average number of Cells counted was 24(DF 2) find out the the number of cells/ml and total number of cells in 7 ml? How many cells you need for 100 wells, assuming Number of cells/well is 10 to the power 4 cells?

What is your overall dilution factor if you complete 3 serial dilutions using a 100-fold dilution each time? (Show your work)

Determine the minimum inhibitory concentration and the minimum exposure time given the following data: Number of colonies of E. coli on Nutrient Agar plates Exposure time (in minutes) Concentration of ethanol 10 15 Distilled water +++ +++ +++ 40% +++ ++ 70% 95% Minimum inhibitory concentration (MIC): Minimum exposure time (MET):

topic: Isoelectric Precipitation   Appearance of the mixtures containing casein mixed with different buffer solutions: (a) TestTube 1 containing Glycine-HCl buffer; (b) Test Tube 2 containing Acetate buffer; and (c) Test Tube 3containing Phosphate buffer. QUESTION: Based on the given results, what do you think is the isoelectric pH of casein? Briefly discuss your basis for determining it.

1&3/4 tbs po BID x 7 days #QS How many milliliters should the pharmacy dispense?

Standard curves You are setting up a standard curve for lysozyme. You have a stock vial containing 2 mg/ml of lysozyme and you have 2 ml in the vial. You will want to have a minimum of 1 ml of each concentration for the spectrophotometer. How would you proceed? (Saline is added to make the various concentrations). Concentration Am't stock lysozyme or am't of a previous dilution (note which) Amount saline Total volume wanted 1.00 mg/ml 0.75 mg/ml 0.50 mg/ml 0.375 mg/ml

Calculate the IV rate in drops per minute for a 1 L bag of Lactated Ringers solution running at 50ml/he using the 15 drop factor?

ent id%= 20737 ning Time: 28 minutes, 53 seconds. ion Completion Status: turned red after Zn Stayed colorless after Zn turned red after NI and NII Which bacteria possess potent (strong) nitrate reductase ? Pseudomonas aeruginosa Staphylococcus epidermidis Streptococcus pneumoniae E.coli dtv MacBook Air O O O C Streptococcus pneumoniae Staphylococcus epidermidis Escherichia coli Psеudomonas aeruginosa

procedure/s in performing aseptic transfer of bacterial cultures in (include illustration) (1) broth culture to broth

Interpretation: > Determine the total number of bacteria per plaque sample and toothbrush head from BHI. Number of colonies on a selected plate CFU/ mL = Total dilution x amount plated Questions: Results of the plates using the plaque sample Q1: Which of the 6 BHI plates -1 10 10 2 shown would be the one to use as your count for the formula? TMTC TMTC TMTC Q2: Set up the formula and solve for # of CFUS per plaque sample. 10 238 colonies 24 colonles 2 colonies

Patch clamp  What is patch clamp technique? Identify two features of the patch clamp technique that make it a useful tool in studying cellular electrophysiology. Explain each thoroughly. What is the role of the reference electrode in the patch clamp technique? Discuss how it works.

in food microbiology, how do you compute for concentration (M) and absorbance (A) of peroxidase activity on hydrogen peroxide at different pH values? (wavelength used = 415nm). can you please explain the calculations thank you    if given:    Temperature = 75 C  Molar absorptivity coefficient = 10.5 Path length (cm) = 2 I = transmitted light = 0.45

Briefly explain that what is the important to use immobilization techniques in biosensor?..(explain under5 points)

1. (2.5 pts) A pure bacterial culture of unknown concentration was diluted to determine the concentration of viable bacteria in the original culture. Serial dilutions were performed as diagrammed below. Each dilution tube contained 400 ul of diluent and 100 ul was transferred into each tube. TSA plates were inoculated with 100 µul from the last three dilution tubes. a. What is the dilution between each tube shown in the diagram below? Express your answer as a ratio. b. What is the total dilution of tube number 5? Express your answer as a ratio. c. What is the concentration of viable bacteria in the original culture? Express your answer using scientific notation and the units CFU/ml. d. What is the concentration of viable bacteria in tube number 2? Express your answer using the units CFU/ml. e. If you inoculated a TSA plate with 250 µl from tube number 5, how many colonies would you expect to see after the plate was incubated? 1 2 3 5 423 80 13 Number of colonies

Sulfur Indole Motility (SIM) Medium H2S produced, color and +/-: ______________________________ Indole present/Tryptophan hydrolysis, color and +/-: ___________________________ Motile or non-motile: _____________________________

Table 1 - Comparison of the effect of catechol concentration on the amount of product formed. Absorbance Potato extract Absorbance 0 mins after 30mins (2nd reading) (mL) 1st reading 1 Tube # la blank 2a 3a 4a 1 1 1 dH₂O Catechol (mL) (mL) 7 5 3 1 0 2 4 6 0.00 0.060 0.033 0-05-2 Q4) Give 2 reasons for adding dH₂O to these tubes in Table 1? Time for reading: 3:21 -0.11 Absorbance: Time for reading: 3.36 Q5) Tube la serves as a control, but why is this control needed? Absorbance: 0.197 Time for reading: 3.37 Based on the data from Table 1 answer these questions: Q1) What is the name of the enzyme found in potato extract? Answer: catechol Q2) What is the substrate? Answer: THO Q3) Name of product of this enzyme catalyzed reaction? Answer: Absorbance: 0.152 Time for reading: 3:39 Absorbance: . 166 ness Catechol Benzoquinone Subtract 1st from 2nd reading -0.01 0-137 0.11.19 0.119 Q6) Notice that your 1st absorbance reading in tubes 2a-4a are quite similar but it then becomes very different…

Infuse heparin at 1,200 units per hour from a solution containing 40,000 units of heparin in 500 mL D5W. How many mL/hr will deliver the ordered dosage?

List the ingredients and recipe (in gram or milligram) to make one liter (1000 ml) of Trypticase Soy Agar, nutrient agar, and Columbia blood medium (use a table

based on the chart answer the following: 1. which proteins will be most excluding use gel filtration chromotagrpahy  2. which proteins will migrate closer to the anode using SDS- PAGE 3. which protein will have the most net positive charge at a ph of 7.4

HW....... Q1/: Compare the contact time necessary to obtain 99.99% kill of bacteria in water under the following conditions: 1- Free iodine residual of 0.45 mg/L and k=1.1* 10²/s. 2- Combined iodine residual of 8 mg/L with k= 1.2* 10²/s.

Experiment Title : Surface Modification of poly(vinylidene fluoride) (PVDF) membranes with untreated and treated chitosan for antibacterial performance. QUESTION: From the given title, please make a clear discussion about antibacterial perfomance

Question: Determine the amount of dehydrated medium needed to prepare 15 butts, 20 slants, and 25 butt-slants. Include amount for 2 additional units of each as excess to compensate for compounding losses.

AE, 62, Male Wt: 75 kg, Ht: 150 cm Orders: Penicillin G Potassium            10,000,000 Units Sterile water for injection                              qs Make a 250,000 U/mL solution.   The package insert states If Penicillin G Potassium 10,000,000 Units is reconstituted with 44 mL sterile water, a solution of 200,000 U per mL will be obtained.   What volume of sterile water would you use to reconstitute the penicillin G potassium in order to make the ordered solution?

a. Let’s say for example that a milk sample has 10,000 bacteria per milliliters. If 1 mL of this sample were plated out, these would theoretically be 10,000 colonies in the Petri plate. Discuss and explain the serial dilutions of this example. b.  The disk diffusion method was used to evaluate 3 disinfectants. The results were as follows: Solution Zone of inhibition X 0 mm Y 5 mm Z 10 mm How would you interpret? Provide inference.

Serial dilution = previous dilution x dilution 11 mL [2] 3 mL Colony count: 100 0.1 mL sample [1] 9.9 mL 5 mL 0.1 ml. Cokony count Dilution: 10-1 10-3 [3] 150 Dilution factor (DF): [4] [5] [6] Compute for the CFU/mL of the sample. Show your solution and express your final answer to 2 significant figures.

Consider the sterilization of a sodium gluconate production medium in the holding section of a continuous sterilizer. Assuming constant temperature, the specific death rate constant of the contaminant is 20 s-1 . If the average residence time in the holding section is 10 seconds, calculate the Del factor for the following and explain the results. i. For Pe = 400 ii. For Pe = 400, assuming plug flow iii. For Pe = 100 iv. For Pe = 100, assuming plug flow

Observe the following Plate counts and then determine the correct number of CFU/ml Plate 1 = 564 colonies at 10^-5 dilution Plate 2 = 422 colonies at 10^-6 dilution Plate 3 = 317 colonies at 10^-7 dilution Plate 4 = 93 colonies at 10^-8 dilution   93 x 10^10 CFU/ml 9.3 x 10^-9 CFU/ml 93 x 10^9 CFU/ml 93x 10^8 CFÜ/ml 93x 10^-8 CFU/ml    asap please