Agar plate

isolated by using the streak plate technique.1 Using aseptic technique, the two nutrient agar streak plates were made and incubate at two different temperatures: 25 C and 37 C.1 The streak plates were incubated for 48 hours before being put into the refrigerator for five days. The streak plates then were observed and characterized based on the two types of colonies on the plates. The plate incubated at 25 C had both lines and dots that were beige and cloudy. The plate incubated at 37 C did have

of our experiments and our names among other information on seven different Agar plates. We then used seven different containers of sterilized water to make sure there was no contamination between different samples. We also utilized seven different disposable swabs for this exact same reason. We also used seven packages of Para-film in order to seal the plates. Lastly we made use of a freezer in order to keep our plates at the proper temperature for bacteria incubation and growth. These are all the

Eosin Methylene-Blue agar used for the growth of Escherichia coli. These bacteria are commonly found in places of fecal contamination (CDC). A control group of Escherichia coli was used as well as swabs from the student’s phone and hand. Another type of media used in this experiment is Differential/Selective media. This media makes detection easier after the incubation period has occurred. An example of differential/selective media used in this experiment was Mannitol Salt agar used to isolated the

solution was added to each microtube, with use of a sterile pipette. Afterwards, the pipette was placed in a bleach solution and the microtubes were placed into the foam rack and onto crushed ice. Next, a colony of bacteria was removed from the starter plate with a sterile loop and thoroughly mixed within the +pGLO microtube. This step was repeated with a second sterile loop and the -pGLO microtube. After their use, all loops were placed in the bleach solution for decontamination and the microtubes were

Microbiology Instructor: Dirk VandePol Date: 6/21/2013 Streak Plate Isolation for Obtaining Pure Culture 1. When an agar plate is inoculated, why is the loop sterilized after the initial inoculation in put on? Ans: We use agar plate to inoculate microbes by zipping the loop on the agar several times. We streak on the agar plate four time, propose is to isolate the unknown bacteria. Therefore, the first time to streak on the plate, there are million of bacteria on the loop. For that reason,

Professor handed out one unknown organism to each of the students. Students had to record their own unknown ID. Used the aseptic techniques to perform the gram stain and inoculate the nutrient media(nutrient broth, nutrient agar slant, and nutrient agar plate). Only for the nutrient agar plate media used four quadrant streak method. Gram stain was distinguishing the gram-positive or gram-negative cells. Preparing of the heat-fixed slide was taking some times while air dries it. Therefore, inoculated the media

this lab. Find each section and complete the “Preparing for Class” sections. Preparing for class - Day 1 Read in your lab manual about the following agar mediums: Blood Agar (pg 168), EMB Agar (pg 170), Mannitol Salt Agar (MSA)(pg 172) ), MacConkey Agar (pg 174), and PEA Agar (pg 176) to answer the following: 1. What does the blood agar select for?

Use the cotton swab to streak the five labeled nutrient agar plates with the E. Coli K12 bacteria from the tube. 36. Remove a tiny bit of E. coli from the tube using the cotton swab. 37. Hold the tweezers with the cotton swab (or just the cotton swab alone if you are using extra-long cotton swabs) in one hand

organisms can easily be seen using differing types of agar, which creates an ideal environment for the organisms to form colonies, which are groups of hundreds of organisms that can be seen with the naked eye. In order to see individual microorganisms, it is necessary to use the magnification of a high-powered microscope. These techniques of microbiology are used in the following five experiments. The first experiment used Trypticase Soy Nutrient Agar (TSA), which can grow a wide variety of organisms

onto the respective sections. The plate was then incubated at 37oC for 48 hours. The process was then repeated on nutrient agar plates with 5, 6.5 and 10% salt. Experiment 5: Effect of oxygen concentration on bacterial growth A fresh pipette was used to transfer 0.5ml of broth culture of E. coli, to be inoculated, into a tube of molten agar previously boiled to drive off oxygen. The tube was then rolled to distribute the bacteria and allowed time for the agar to harden. The tube was incubated