You have an aqueous solution of hemoglobin (pl = 6.8) at pH 6.5, and currently the proteins are soluble. If you had 1 M HCl and 1M NaOH at your disposal, what could you do to precipitate the albumin out of solution? O add NaOH to the solution O add HCl to the solution add NaOH to the solution, then add equal volume of HCl to the solution O add HCl to the solution, then add equal volume of NaOH to the solution An error has been detected in your answer. Check for typos, miscalculations etc. before submitting your answer. Submit Answer Try Another Version 1 item attempt remaining
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- You have an aqueous solution of hemoglobin (pl = 6.8) at pH 5.2, and currently the proteins are soluble. If you had 1M HCl and 1M NaOH at your disposal, what could you do to precipitate the albumin out of solution? add NaOH to the solution O add HCl to the solution O add NaOH to the solution, then add equal volume of HCl to the solution O add HCl to the solution, then add equal volume of NaOH to the solutionYou have been observing an insect that defends itself from enemies by secreting a caustic liquid. Analysis of the liquid shows it to have a total concentration of formate plus formic acid (K₁ = 1.8 x 10-4) of 1.45 M. Further analysis reveals that the concentration of formate ion is 0.015 M. What is the pH of the secretion? pH =1.0.1 mL of a protein solution of concentration of 11 mg/mL was diluted to a total volume of 4.0 mL with water (i.e. 0.1 mL of the solution was added to 3.9 mL of water). 2 mL of this solution was then mixed with 18 mL of water. What is the concentration of the diluted protein solution? Space to show your workings:
- A mixture of proteins contains four different polypeptides, all in ~equal concentration, in solution with the following properties: Protein Molecular Mass (kDa) Isoelectric point A 45 4.5 B 77 6.0 C 28 4.1 D 14 10.7 A fraction of the protein solution is applied to a strong cation exchange column using a buffer at pH 8.0 with increasing [NaCl] from 0.05 M – 1.0 M. The chromatogram is shown below: 1. Based on the data presented, which of the following statements is true: Peak #1 is protein D Peak #4 is protein C Peak #3 is protein A Peak #4 is protein D Peak #2 is protein B 2. Since you know that the proteins are all present in approximately equal concentrations, the different relative peak areas tell you that: There are more neutral amino acids in protein #4…Polymer beads (resin) made of DEAE (diethylaminoethyl) cellulose are packed in an ion exchange column. The total mass of beads in the column is 8.47 kg. On average, each bead weighs 0.0023 g and has an average of 18.4 * 10° positively charged amine groups that can adsorba negatively charged protein that passes through the column. A solution containing 2.07 mg/L of a protein is maintained at pH 6.3 and is passed through the ion exchange column at 0.215 L/min. The protein has a molecular weight of 154,000. The pk, of the amino groups on DEAE cellulose is 7.1, and the pl of the protein is 5.6. 2. A. How long can the column be operated before reaching 80% capacity (i.e., 80% of the amino groups on DEAE are bound to the protein through an ionic bond)? You may assume that one protein attaches to one + charge on the beads (although it's possible that proteins attach to more than one + charge). B. After reaching 80% capacity, explain what you would do to release the protein attached to the…At what pH does a lysine solution exhibit the highest buffering capacity? (Lysine pKas: pk1 = 2.2, pK2 = 8.95, pK3 = 10.5) %3D O рH 12.1 О рH 9.72 O pH 5.67 pH 8.95
- 3. 4. A protein solution is prepared by dissolving 800 µg of protein in 200 µL of water. A 150 μL sample of this solution is diluted to a total volume of 4.5 mL. How many mg of protein will be in a 3 mL sample of the diluted protein solution? Space to show your workings:Given a stock protein solution with a concentration of 3 mg/ml, determine the protein concentration of a solution made by mixing 5 μl of the stock with 5 μl of a buffer.What is the pH of a buffer of a buffer system that contains a weak acid of .19 M and conjugate base of .12 M ? The Ka of the acid is 5.6x 10 -6 pH= pKa + log ( [A- ]/[HA]) please show me step by step
- People often take milk of magnesia to reduce the discomfort as- sociated with acid stomach or heartburn. The recommended dose is 1 teaspoon, which contains 4.00 x 102 mg of Mg(OH)2. What volume of an HCI solution with a pH of 1.3 can be neutralized by one dose of milk of magnesia? If the stomach contains 2.00 x 102 ml. of pH 1.3 solution, is all the acid neutralized? If not, what fraction is neutralized?You have a mixture of 4 amino acids (Q, W, E, R, T) that you want to separate using ion exchange chromatography. Which of these amino acids would bind most strongly to a DEAE anion exchange column at pH 7? Q W E R TIn a 0.1000 M acetic acid solution at 25 degrees celsius , the acid ionizes to the extent of about 1.34 %. Since each molecule of acetic acid which ionizes produces 1 H+ ion and 1 C2H3O2- ion, the concentration in the solution are: HC2H3O2 < -----------> H+ + C2H3O2-