You are trying to clone you favorite piece of DNA into the PUC vector shown above. You've pulled an all nighter, unfortunately binge-watching the entire season of the Mandolorian on Disney+. You digested the vector/plasmid with Sal1 endonuclease and your piece of DNA (insert) with both Sal1 and Xho1. Part A) After ligation and transformation, you grow your bacteria cells on plates containing Kanamycin. How many colonies do you expect to grow on the plates? O 100 O 75 O 50
Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
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