You are doing a genetic engineering experiment. You use restrictions enzymes to cut the regulatory sequences from the lac operon and replace them with the regulatory sequences of the trp operon. Specifically, you will eliminate everything upstream of the beginning of lacZ and replace them with the trp sequences upstream of the beginning of trpE, including trpR. Now, describe the regulation of your new constructed gene. What will you do to get expression of the three lac genes?
You are doing a genetic engineering experiment. You use restrictions enzymes to cut the regulatory sequences from the lac operon and replace them with the regulatory sequences of the trp operon. Specifically, you will eliminate everything upstream of the beginning of lacZ and replace them with the trp sequences upstream of the beginning of trpE, including trpR. Now, describe the regulation of your new constructed gene. What will you do to get expression of the three lac genes?
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Gene Interactions
When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.
Gene Expression
Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.
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Transcribed Image Text:Question 5A
You are doing a genetic engineering experiment. You use restrictions enzymes to cut the regulatory
sequences from the lac operon and replace them with the regulatory sequences of the trp operon.
Specifically, you will eliminate everything upstream of the beginning of lacZ and replace them with
the trp sequences upstream of the beginning of trpE, including trpR. Now, describe the regulation of
your ñew constructed gene. What will you do to get expression of the three lac genes?
The lac Operon and its Control Elements
lacl
CAP PO
lacz
lacY
lacA
genes
5
binding
site
3
DNA
AUG
AUG
AUG
messenger RNA
RNA polymerase blocked from
transcribing trp operon
Regulatory gene
trp operon
DNA
PR
trpR
Ptrp
trpE
trpD
trpC
trpB
trpA
Repressor bound
to operator
Promoter
5'
3'
trpR-MRNA
(a) Tryptophan present, repressor bound to operator, operon ropressed.
When complexed with tryptophan, the repressor protein produced by the
trpR gene binds tightly to the trp operator, thereby preventing RNA
polymerase from transcribing the operon genes.
Inactive form
Active form
T.
of repressor
of repressor
Tryptophan
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