Write a short description of the physical characteristics of acid & enzymatic hydrolysates. What will be the result of both hydrolysates for Benedict's test(positive or negative and state what color will it become)
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Write a short description of the physical characteristics of acid & enzymatic hydrolysates. What will be the result of both hydrolysates for Benedict's test(positive or negative and state what color will it become)
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- Based on this video https://www.youtube.com/watch?v=rKng5-ij6kQ Provide a schematic diagram for the Iodine test methodologies in determining the presence of carbohydrates. Also, give the basic principle for the test.What test reagent is most sensitive in detecting sugar in urine? What are the possible positive results (color) and its interpretation? What test could be used to differentiate: sucrose and lactose? Explain. glucose and starch? Explain. glucose and fructose? Explain. galactose and fructose? Explain An unknown carbohydrate gave a brick red precipitate when tested with Fehling's reagent, turned red when reacted with Seliwanoff's reagent, and quickly gave a red precipitate when reacted with Barfoed's reagent. What conclusions can be made about this carbohydrate? Illustrate with equation the reaction of glucose, galactose, maltose & lactose with osazone test. Sketch the crystals formed for each of the following sugars examined under the microscope.Based on this video https://www.youtube.com/watch?v=rKng5-ij6kQ Provide a schematic diagram for the Barfoed’s test methodologies in determining the presence of carbohydrates. Also, give the basic principle for the test. (not a graded question, video is very brief)
- An amino acid mixture consisting of lysine, leucine, and glutamic acid is to be separated by ion-exchange chromatography, using a cationexchange resin at pH 3.5, with the eluting buffer at the same pH. Which of these amino acids will be eluted from the column first? Will any other treatment be needed to elute one of these amino acids from the column?Based on this video https://www.youtube.com/watch?v=rKng5-ij6kQ Provide a schematic diagram for the Seliwanoff’s test methodologies in determining the presence of carbohydrates. Also, give the basic principle for the test.Based on this video https://www.youtube.com/watch?v=rKng5-ij6kQ Provide a schematic diagram for the Fehling’s test methodologies in determining the presence of carbohydrates. Also, give the basic principle for the test. (not a graded question, video is very brief)
- Based on this video https://www.youtube.com/watch?v=rKng5-ij6kQ Provide a schematic diagram for the Barfoed’s, test methodologies in determining the presence of carbohydrates. Also, give the basic principle for the test.In Fehling’s test, aldose are oxidized to aldonic acid. Kindly give the reaction and mechanism of Fehling’s test from glucose to gluconic acid and then to the positive result. What would the reaction be if the sample is a non-reducing sugar?Why is ascorbic acid regarded as an antioxidant and What is the basis of the ascorbic acid saturation test
- An amino acid mixture consisting of lysine,leucine, and glutamic acid is to be separated by ion-exchangechromatography, using a cation-exchange resin at pH 3.5, with theeluting buffer at the same pH. Which of these amino acids will beeluted from the column first? Will any other treatment be neededto elute one of these amino acids from the column?Illustrate with an equation the reaction of glucose, galactose, maltose & lactose with osazone test. Sketch the crystals formed for each of the following sugars examined under the microscope. What test reagent is most sensitive in detecting sugar in the urine? What are the possible color positive results and their interpretation?Using DEAE-cellulose as ion exchange resin, indicate the starting and ending pH for the narrowest experimental pH range used to separate an amino acid mixture consisting of Gln, Leu and Lys Starting pH: _____ Ending pH: _____