Why is it that somatic gene therapy is allowed in many countries and yet germ-line gene therapy is often restricted? What are some arguments for and against germ-line gene therapy?
Q: What is a simple way of making a knockout mouse in terms of DNA recombinant technology?
A: A knockout mouse, or knock-out mouse, is a genetically modified mouse in which researchers have…
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Q: why is the tas2r38 gene also called the ptc gene
A: PTC is a compound known as Phenylthiocarbamide (PTC), also called phenylthiourea (PTU). It is an…
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Q: In the gene therapy trials for SCIDS, what DNA was added to the patients’ cells? Could this same…
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Why is it that somatic gene therapy is allowed in many countries and
yet germ-line gene therapy is often restricted? What are some
arguments for and against germ-line gene therapy?
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- which of the following statements are false? Drugs such as Ataluren should be effective in treating all types of Pax6 mutations Gene therapy stategies aimed at over production of Pax6 should be an effective strategy for Aniridia treatment The target protector strategy developed by the Howard lab for Aniridia treatment is aimed at increasing the expression of the mutant allele of Pax6 all of the above none of the aboveWhat is the difference between somatic gene therapy and germ-line gene therapy?In the gene therapy trials for SCIDS, what DNA was added to the patients’ cells? Could this same procedure be used to treat a dominant disease – why or why not? What are some other options?
- A couple with a child affected with DBA undergoes in vitro fertilization (IVF) and genetic testing of the resulting embryos to ensure that the embryos will not have DBA. However, they also want the embryos screened to ensure that the one implanted can serve as a suitable donor for their existing child. Their plan is to have stem cells from the umbilical cord of the new baby transplanted to their existing child with DBA, thereby curing the condition. What are the ethical pros and cons of this situation?Breast Cancer Screening Program – A group of women were subjected to a breast cancer screening program. A newly developed screening test was utilized to measure its sensitivity and specificity against a gold standard test. a) What is the prevalence of breast cancer in this population? b) Calculate the Sensitivity of the test and provide interpretation c) Calculate the Specificity of the test and provide interpretationWhy do we need to work out ALTERNATIVE V-STEP? What are the benefits?
- What are the implications of resistance gene transfer for the future practiceof medicine?Cystic fibrosis (CF) is a genetic disorder affecting a number of organs, including the lung airways, pancreas, and sweat glands. Mutations in both copies of the CFTR gene causes cystic fibrosis. Imagine that you have sweat gland samples from several Cystic Fibrosis patients (A-C) with unknown mutations in CFTR. You also have normal (+) sweat gland sample to use as a positive control. А В С А В С Choose which mutation would explain the RNA and protein results in A, B, & C: 1. Promoter/Regulatory mutation 2. Silent mutation 3. Missense mutation 4. Deletion mutation 5. Splice site mutation 6. Nonsense mutation RNA gel Protein gelThe p53 protein was discovered through its association with SV40 T antigen and assumed initially to be an oncoprotein. a. What is the current consensus as the function of p53 and what evidence caused this change in view? b. How does the effect of mutation in the p53 gene differ from the effect of mutation in the RB gene what is the molecular basis for this difference ?
- What is oncogenic transformation ?Which vector is used to replace the defective gene in gene therapy?Do all of them True/False 31) The process by which an electrical charge is used to introduce DNA into a cell to produce a transgenic organism is called electroporation.Answer: 32) Reproductive cloning is used to produce large amounts of mammalian proteins from transgenic agricultural animals such as cattle.Answer: 33) In gene addition, homologous recombination is used to remove the original gene and replace it with the cloned gene.Answer: 34) All stem cells have the potential to differentiateAnswer: 35) A bone marrow transplant involves the transfer of multipotent stem cellsAnswer: 36) The fact that in mammalian systems multiple genes may compensate for the loss of a gene is called gene redundancy.Answer: