Why is it not recommended to use reconstituted or evaporated milk? Why is full cream milk suggested to be used?

Q: Based on the figure and the explanation in the textbook, what are the random chances that the…

A: Denaturation is the process by which proteins lose their native conformation. Ribonuclease A has 124…

Q: You have prepared a BSA serial dilution (ug ml) to construct a protein standard curve, for which the…

A: The standard equation for calculating concentration is Beer Lambert's law, A= ebc, where A is…

Q: By increasing the percentage of acrylamide in an SDS-PAGE gel, one will observe the following change…

A: Macromolecules are separated and analyzed by using electrophoresis. Macromolecules get separated on…

Q: Which nuclear isotope used in protein NMR spectroscopy is the most sensitive to detect? Briefly…

A: NMR Spectroscopy is used to study the structure and composition of biomolecules like proteins. Here…

Q: Write down the values in bold print. You are isolating a protein with a pl of 8.5 using cation…

A: As given, protein of interest has pI of 8.5 which was isolated using cation exchange chromatography.…

Q: C

A: This question can only be answered hypothetically by taking the standard Rf values of some polar…

Q: As an example, consider albumin, a protein made of a single polypeptide weighing 66,000 daltons (66…

A: Gel electrophoresis if the technique of separation of molecules on the basis of their size and…

Q: An allosteric enzyme is purified and determination of its mass by gel-filtration chromatography…

A: Enzymes are a class of proteins that functions to catalyze multiple reactions. Protein, on the other…

Q: Why is sucrose a non-reducing sugar with Benedict’s solution while lactose is a reducing sugar under…

A: Benedict's reagent  is a chemical reagent  Sodium carbonate, sodium citrate, and copper sulfate…

Q: How does cell chemistry, and therefore all life, depend on the production of proteins? What role do…

A: Proteins are large-complex molecules that perform complex and critical tasks in all life forms.…

Q: What is the concepts of the native conformation of proteins? Why and how do proteins refold and…

A: Proteins are polypeptide structures consisting of one or more long-chain amino acid residues. They…

Q: The dye used to complex with the proteins is : * Coomassie Brilliant Blue Dye G-250 O Bromophenol…

A: A large number of dyes are present commercially for staining proteins, some are costly and some are…

Q: Are macrolides more effective in blocking the synthesis of small or large proteins? Why?

A: A drug class that includes several antibiotics like Azithromycin, Clarithromycin, and Fidoximycin is…

Q: Describe in detail the vapour diffusion method for crystallising a protein

A: Vapor diffusion is the most typically employed method of protein crystallization. In this method,…

Q: Proteins such as enzymes can be denatured by high temperatures or environments with extreme pH…

A: Proteins are linear polymers made up of amino acid residues. Cells, tissues, and organs of organisms…

Q: What is protein denaturation? Is there any change in the primary structure when a protein is…

A: Proteins are the molecular tools, which perform an astonishing variety of functions. Moreover to…

Q: You want to treat 10 mL of HL-60 cells with cycloheximide in order to determine the half-life of a…

A: According to Normality Equation,

Q: In the Procedure, you were instructed to use your original mixture of phycocyanin and 0.10 M sodium…

A: Phycocyanin: It is a blue color pigment found mainly in cyanobacteria and some algae and is…

Q: Suppose you have a mixture of the following proteins protein A: pl = 3.5, mw = 35 kDa protein B: pl…

A: Cation exchange chromatography is defined as part of the ion-exchange chromatography. It is used to…

Q: Use three diagrams to illustrate the separation of three different proteins by the method of…

A: Ion-exchange chromatography is an analytical technique that plays a vital role in separation of…

Q: Using the chart below, can you produce a two-step procedure that demonstrates protein purification…

A: Proteins are polymers of amino acids linked by peptide or amide bonds. Charge on the protein depends…

Q: What type of protein is included in milk? When milk sourness occurs, why does this protein…

A: Proteins are a linear chain of amino acid sequences attached together via peptide bonds. Proteins…

Q: The addition of ethanol, CH3CHOH, t an aqueous solution lowers the surface tension of the solution.…

A: Water is a universal solvent. It can dissolve many substances in it. It is essential for every…

Q: Five amino acids were subjected to normal phase chromatography, and the folkowing Rf val were…

A: Chromatography is the technique of separation of components of a mixture based on their physical…

Q: At what level of protein structure (primary, secondary, tertiary, or quaternary) will protein…

A: There are various levels of protein structure- Primary structure of the proteins consists of its…

Q: Please provide a chromatography technique to isolate the protein A from the mixture containing…

A: Chromatography is the technique used for the separation of molecules or analytes from the mixture by…

Q: What is the Beer-Lambert’s Law? Why is it relevant to the quantitative analysis of proteins?

A: The Beer-Lambert Law is given by It is a relationship between the absorption of light energy by a…

Q: Explain the difference between essential and nonessential amino acids. How many of each are there?…

A: Proteins are one of the macronutrients present in the food. They are very essential for growth and…

Q: You have a soluble protein that is highly flexible and is only 23 kDa in size. What is the most…

A: Proteins are the polymers of amino acids which are arranged in the three-dimensional structure of…

Q: If you incubate a protein with a single beta sheet in solution at pH = 14, the beta sheet no longer…

A: Proteins are essential for the normal functioning of the body. They are involved in providing…

Q: Which of the following might cause the denaturation of a protein? Select all that apply.     A.…

A: PROTEIN STRUCTURE:- Protein structure is a 3 D Structure which forms 4 structures: 1)PRIMARY…

Q: simple proteins that generally yield proline upon hydrolysis. these proteins are solubler in 70-80%…

A: Proteins are the macromolecules composed of amino acids. The amino acids are bonded to each other…

Q: You have isolated a protein and use size exclusion chromatography to estimate its molecular mass to…

A: Introduction Chromatography is a scientific technique for separating the components of a mixture.…

Q: In cation exchange chromatography, positively charged proteins exit the column first. In anion…

A: Chromatography is a technique in which components of a mixture are separated. It consist of a mobile…

Q: Hydrochloric acid, ethanol, acetone, nitric acid and picric acid are an agents of denaturation.…

A: A polypeptide protein is properly folded then only it is functional, If any change in its…

Q: Your vaccine will be administered as a topical cream, and you require your peptide to have an…

A: A topical drug is one that is applied to a specific area of the body or to a specific organ. Topical…

Q: Five amino acids were subjected to normal phase chromatography, and the following Rf values were…

A: Retention factors are useful for the comparison of the results of chromatographic separation of one…

Q: Amino acids are ionic compounds, very soluble in water. Proteins, however, which are made up of…

A: Proteins are macronutrients that are made up of amino acids. Amino acids are organic molecules that…

Q: What is the molecular weight of the Botulinum neurotoxin, a protein that contains 1350 amino acids?…

A: Given Values: Number of residues present in botulinum toxin = 1350

Q: When a protein denatures in a cell, it tends to assemble into aggregates. Propose an explanation for…

A: Proteins have distinct structures. If they are exposed to specific conditions, they may lose their…

Q: Our body is compost of about 70% water which means that majority of the chemical processes happens…

A: The amino acids are classified based on the polarity as, Polar amino acids (hydrophilic)- These are…

Q: Given the following peptides below, what would be more soluble at the indicated pH? 1. (Gly)20 or…

A: Peptides are composed of twenty standard amino acids attached through peptide bonds. These twenty…

Q: You are given a protein solution with a concentration of 0.15 mg/ml. Suppose that we want to prepare…

A: An analyte must be present in detectable concentrations. Depending on the requirements of the…

Q: Determine which of the samples should be extracted and separated for protein analysis based on the…

A: Protein analysis methods encompass a wide range of experimental techniques for protein detection,…

Q: Which of the following is TRUE, if the protein  pH is electrically neutral? Refer to the graph…

A: Protein denaturation is the process where protein loses its secondary conformation due to extreme…

Q: Consider the following properties of the protein components of a sample mixture as provided in the…

A: Proteins are composed of twenty standard amino acids attached together via peptide bonds. These…

Q: Which of the following is NOT a unit of concentration?     All of these options are units of…

A: The specific activity is the activity of the enzyme present per milligram of the total enzyme.

Q: Which of the following techniques takes advantage of NET CHARGE to purify/characterize proteins? a.…

A: The monomeric units of the polypeptide chains are amino acids. They form peptide linkages to form…

Q: Draw the Fischer Projection, Haworth and Chair conformation for D-Glucose

A: Carbohydrate sugars are present in several forms. These conformations are either open in a chain…