Why is fumarate hydrated? To enable it to interact with an enzyme O To form a double bond To reduce water present in the cell To reform oxaloacetate To prepare an alcohol for oxidation
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![Question 28
Why is fumarate hydrated?
To enable it to interact with an enzyme
O To form a double bond
To reduce water present in the cell
To reform oxaloacetate
To
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- QUESTION 10 Which one of the following statements does NOT correctly describe the current state of the chymotrypsin-substrate complex below or the next mechanistic step that occurs? s,' G193 S195 NH NH R -NH R-NH HN- Ser-195 His-57 HN Asp-102 OA. This intermediate is part of a nucleophilic acyl substitution reaction. OB. In the next step His-57 protonates Ser-195. O C. In the next step His-57 activates a water molecule. O D. In the next step His-57 protonates the leaving group. OE. In the next step the N-terminal fragment of the original substrate departs.Question 2. An enzyme was found to convert Acetyl-coA to an acetyl-cysteine intermediate of the enzyme. The structure of cOA is: H H НО СН; HS — СH2— СH2 — N—C—CH,— CH2—N- С—С—С—CH;—0—Р- 0–CH2 Adenine н CH H H H Phosphopantetheine group of coenzyme A 2-O3PO ОН Draw a plausible chemical mechanism. Where is the acetyl-group in acetyl-coA? What is the reactive group of the protein? The reaction is enhanced at higher pH. How is this consistent with your mechanism? Is covalent catalysis involved?Question 7 of 16 ©Mat One way of expressing the rate at which an enzyme can catalyze a reaction is to state its turnover number. The turnover number is the maximum number of substrate molecules that can be acted on by one molecule of enzyme per unit of time. The table gives the turnover number of four representative enzymes. W F2 #m 3 E How many molecules of fumarate can one molecule of fumarase act on in 29.0 min? D fumarate Lactate dehydrogenase lactate fumarate molecules: F3 > Enzyme Ribonuclease Fumarase $ 4 R Urease F F4 % 5 T G B 6 HOLO F5 Substrate Turnover number (per second) RNA 100 800 1000 10.000 Y H & 7 urca F6 U J DELL * 8 CO F7 K ( 9 FB O P ; man Wi F10 [ F11 1 F12 Backspace Enter Insert Print Screen Shift Delete Home Scroll Lock Answer End 8:24 PM 10/15/2023 + PgUp Pause Break PgDn Num Lock 7 Home 4 1 Enc
- QUESTION 4 What TYPE of inhibition is observed in the following: S E Competitive Non-competitive Uncompetitive AllostericQUESTION 1 Which of the following concepts in column A best fits the concept in column B. p-Nitrophenyl a-D-Glucoside Action of intracellular a-GLUCOSIDASE + NaHCO3 Boiling cells Tris-pH 5.0 A. Substance that act as molecular mimic of a maltose molecule B. Develops TT-Nitrophenyl into a yellow colour reagent for measurement at 400nm c. Breaks the a-linkages of p-Nitrophenyl a-D-Glucoside, leaving p-Nitrophenyl and a glucose molecule D. Allows transport of p-Nitrophenyl a-D-Glucoside across the membrane E. Stop the reaction and release internal p-NitrophenylQuestion 4 If 32p – labeled (radioactive phosphate) inorganic phosphate were introduced to erythrocytes undergoing glycolysis which of the following molecules would you expect to detect 32 p in Glycolytic intermediates? Select an answer and submit. For keyboard navigation, use the up/down arrow keys to select an answer. a Fructose 1-6 bisphosphate b 3-Phosphoglyceraldehyde Dihydroxyacetone phosphate d 2-Phosphoglycerate e 1-3 bisphosphoglycerate f Phosphoenolpyruvate 3-Phosphoglycerate h Pyruvate i None of the above
- Question 36 What type of reactions is represented below? CH2OH CH2OH C=0 H-C-OH HO-C-H HO-C-H CH2OH CH2OH O oxidation of a secondary alcohol O oxidation of a primary alcohol O oxidation of a ketotetrose O reduction of a ketotetroseQuestion 9 If 14CO2 (radioactive carbon) were incorporated into the TCA cycle via the Pyruvate Carboxylase reaction which of the following molecules would contain radioactive carbon? O Multiple answers: Multiple answers are accepted for this question Select one or more answers and submit. For keyboard navigation. SHOW MORE V a Oxaloacetate b Citric acid Isocitric acid d a-Ketoglutarate e Succinyl-CoA f Succinate Fumarate h MalateIncorrect Question 5 The active site of a protein has a shape that most nearly fits None of the above Substrates Products Transition state
- QUESTION 28 Which amino acid is most likely to break an alpha helix? Alanine Isoleucine none of the choices are correct Proline QUESTION 29 If an enzyme has a kcat = 3.3e's-1, Km = 5.9e M, what is its catalytic efficiency? Give your answer in 2 significant figures and scientific notation QUESTION 30 To determine the molecular weight of a purified protein you subject it to ESI mass spectrometry at pH=2. The spectrum i t aiahbeni aln hain m tian Click Save and Submit to save and submit. Click Save All Answers to save all ansuers. 786 Mostly cloudy 9 Type here to searchQuestion 25 Based on the structures of some polysaccharides studied, which of the following is its main function in animals and plants? O Sructural support O Energy storage O Cellular communication and recognition O All of the aboveQuestion 1. Put away your notes and text and draw the complete Calvin-Benson cycle starting from 3molecules of Ru1,5BP. Show stoichiometry along the way using 6 GAP molecules to regeneratethe 3 Ru1,5BPs. Label each reaction according to its enzyme class (your choices arecarboxylase, kinase, dehydrogenase, aldolase, epimerase, isomerase, phosphatase, andtransketolase – see the posted enzyme guide for help on this).
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