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- Why does uninoculated agar plates still has visible growth of microorganisms? Where do you think the microorganisms came from?What is the difference between the pour plate method and the spread plate method? Why might you choose one over the other? What kind of plate must this be and why do you say that?why is it important that you disinfect your work area both before and after working with bacteria?
- How to prepare an agar plate for bacteria? How to include the antibiotics into agar plates? How long should you wait for bacterial colonies to grow on agar plates? What is the appropriate temperature?.What are the possible causes of having Too Numerous To Count (TNTC) bacterial colonies in an agar plate? What are the ways in which this can be prevented?What would your final dilution be if you transferred 0.1 mL of a 10-6 dilution to a sterile petri dish and then added molten agar to make a pour plate? O 1) 106 O 2) 10-7 O 3) 107 O4) 4) 10-5 O 5) 10-6
- Why is dilution a necessary part of pure culture preparation?In the preparation of a bacterial smear, why is there a need to fix the bacteria to the slide? Aside from passing the slide over a flame, what are the other ways of fixing the bacteria to the slide?Abigail did not dry the top of her agar plate and stored it top up. Now she wants to count colonies but there is a smear over the entire plate. She was working with E. coli. Why does she have a smear?
- A student needed to transfer bacteria from a broth culture to an agar plate. Below is the step-by-step what was done to accomplish this. The transfer of bacteria was not successful because of which step? 1. Cap of the broth culture is removed 2. The mouth of the bottle is flamed 3. The loop was flamed 4. The loop was inserted into the culture to pick up the bacteria 5. The loop was flamed 6. The loop containing the bacteria was used to introduced to spread the agar plate 7. The plate was placed in an incubator at 30 CelciusWhy should your solvent be evaporated from the sample placed on the sterile disk before you place it your petri dish?What is the purpose of the condenser? When using a petri dish, How do you label it? What side do you label it on and why? How do you incubate it and why? Where do you dispose the plate when you are done