What is the most commonly used fixative in stool samples and why? Why is staining of stool samples very important in identifying parasites? Why is direct fecal smear a routine procedure in the parasitology section?
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- What is the most commonly used fixative in stool samples and why?
- Why is staining of stool samples very important in identifying
parasites ? - Why is direct fecal smear a routine procedure in the parasitology section?
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- What are the possible causes of having Too Numerous To Count (TNTC) bacterial colonies in an agar plate? What are the ways in which this can be prevented?In the preparation of a bacterial smear, why is there a need to fix the bacteria to the slide? Aside from passing the slide over a flame, what are the other ways of fixing the bacteria to the slide?How do eosin-methylene blue (EMB) agar plates work? What organism(s) are they designed to detect? How would a positive test appear on the plate?
- What is the most commonly used fixative in stool samples and why?What is the purpose of flaming an inoculating loop? How will you know when you have flamed the loop or needle long enough? Why is it necessary to cool the inoculating loop prior to obtaining the bacterial sample? In which direction should you move the inoculating loop in the Bunsen burner flame? (i.e. from the handle to the loop or from the loop to the handle)List five (10) sites on a patient from which potential pathogens could be isolated and prepared for staining and microscopic identification.
- What are some factors used to determine the BSL necessary for working with a specific pathogen? What is the difference between sterilization and aseptic technique?Plastic pipet tips for micropipettes are commonly supplied in packs of 100’s. Once the pack has been opened, contamination is assumed. How can sterility of the pipet tips be ensured prior to use in microbiological procedures? METHOD:RATIONALE:Why is direct fecal smear a routine procedure in the parasitology section?
- Of what practical importance are air borne microorganisms to the laboratory workers? What precautions should be taken to control laboratory contaminants? Why are petri dishes incubated in an inverted position? Of what advantage is the using a solid and a liquid medium?Aside from Giemsa, what other stains could be used in staining blood for blood parasites?What is the appearance of the different blood cells and parasites upon application of the stain?You are hired in one of the microbiology labs in Memphis and your first assignment to identify microbial samples from a local restaurant. You are given a set of slides and asked to classify the specimens as Gram positive or Gram negative. 6) What would be the last reagent you use to identify the bacteria? 7) What type of visual aid you need to help you see the bacterial samples clearly? 8) After the use of what reagent, you will be able to decide if you have a Gram positive or Gram negative bacteria? **Please give the answer**
