What is the EFFECT and RATIONAL of Molten agar mixed with bacterial suspension has temperature higher than 45 oC.

Q: A specific heat treatment used on a particular bacterial suspension has a DRT of 10 seconds, how…

A: Heat treatments have been used for the reduction of bacterial numbers and even for sterilization…

Q: You aseptically transfer 1 mL of your original liquid culture into 99 mL of sterile water. What is…

A: The dilution factor is a term used to describe the ratio of the final volume over the aliquot…

Q: Agar is a solidifying agent used in media preparation. What makes it ideal for cultivation of…

A: Agar is a natural polysaccharide. It is mostly used in the lab and acts as a solidified agent. It…

Q: Before inoculating and pouring molten nutrient agar into a plate, why must the agar first be cooled…

A: Agar or agar-agar is a jelly-like substances obtained from red algae. Agar is a solidifying agent…

Q: 300 +0,005 mg/mL - 0,010 mg/mL - 0,015 mg/mL +0,020 mg/mL 0,025 mg/mL + 0,030 mg/mL -0,035 mg/mL 250…

A: Microbial Growth Curve: The number of living cells in a bacterial population over time is…

Q: Why should the use of nichrome or iron containing loop/wire be avoided as an inoculating device when…

A: The oxidase is a test used in microbiology to identify bacteria that produce cytochrome c oxidase.…

Q: What are the advantages of the API 20E test compared to regular biochemical tube media

A: API 20E test helps in the determination of bacteria from lab-scale activated sludge treatment…

Q: Kligler’s iron agar and SIM are multiple test media.. What component of both media allows you to…

A: Kligler’s Iron Agar (KIA) test is used in differentiating certain members of the Enterobacteriaceae…

Q: Agar is a solidifying agent used in media preparation. What is its origin?

A: Agar gel is a polysaccharide which can become liquefied at temperatures ranges from 85°C to 90°C and…

Q: how do microorganisms such as bacteria eliminate harmful reactive oxygen species?

A: Reactive oxygen species (ROS) are emerging as important elements in the bacterial response to lethal…

Q: What concentration of agar is used in a semisolid medium for motility determination? How does that…

A: Agar is a polysaccharide that is obtained from marine seaweeds. Agar is mostly used in food and…

Q: Is the Triple-Sugar Iron Agar (TSIA) a complex or defined medium? Explain based on its composition.…

A: A growth medium is a also known as a culture medium which can be solid, liquid, and even…

Q: How does the concentration of H+ change when a culturemedium at pH 5 is adjusted to pH 9?

A: pH is the measure of acidity or basicity of a solution. If pH of a solution is 7, then it is…

Q: ould be the effect on your judement of a Gram reaction of an organism if you stain a thick smear…

A: Staining is a process of the well-known structure of the microscopy technique for enhancing contrast…

Q: Calculate the amount of phycocyanin in Sample 1 in mg where A620=0.211   and A650=0.086, taking into…

A: Phycocyanin is a protein-pigment complex that belongs to the family of phycobiliprotein. It is a…

Q: What is the pH indicator in the mannitol salt agar plate? O Bromcresol purple O Kovac's reagent O…

A: For isolating pathogenic staphylococci from samples, cosmetics, and microbial limit tests, Mannitol…

Q: Nitrate reduction to nitrite is indicated by the formation of a pink to red colour after addition of…

A: Sulfanilic acid reacts with nitrous acid to form diazotized sulfanilic acid if introduced. This…

Q: Distinguish between acid and rennet curds.

A: Curdling is the process through which curd is obtained by coagulating milk. The coagulation can be…

Q: 50 mg/50 ml kinetin stock is available  You need 2.5 mg/l kn for Ms media  calculate amount of kn is…

A: A culture medium is a liquid or semi-solid mixture of various nutrients that support microorganism…

Q: Why does turbidity in a cultured media losses its reliability at high cell concentrations?

A: turbidity is used as a measure of cell density within a culture sample.

Q: Compare and contrast use dilution and disk diffusion methods of evaluating disinfectants. Please…

A: Chemical disinfectants are gathered by the sorts of organisms and infectious agents they are…

Q: In casein hydrolysis what is the differance in weak + and strong+ reaction on the agar plate? Would…

A: The enzyme caseinase is secreted into the external media from the cells (an exoenzyme), catalyzing…

Q: Calculate the concentration of the overnight stock culture in CFU/mL

A: Colony-Forming Units (CFUs) refer to the number of individual colonies of any microorganism that…

Q: can the phenol red test also be used to determine if a certain bacterium can metabolze various…

A: Differential media is a type of growth media used in the labs to grow and differentiate or…

Q: What is the use of hay infusion in determination of microbial motility?

A: Hay infusion use in determination of microbial motility.

Q: What is differential medium

A: Differential medium or indicative media contain compounds which support the growth of microbes and…

Q: Eosin Y Test solution

A: Eosin Y- test is a test that is used to check sperm health. It helps to check the intactness of the…

Q: Why is a GasPak anaerobic jar necessary for the culture of anaerobes on Brewer’s anaerobic agar…

A: Brewer’s anaerobic agar and fluid thioglycollate medium (FTM) are used to grow bacteria. Both of…

Q: Composition (+) of reagents Test Importance of the test Group Result Responsible

A: Proteins are macromolecules formed by amino acids; they are essentially polymers of structural units…

Q: What are the purpose of the sample containing albumin, glucose/fructose and boiled starxh solution…

A: A chemical reaction is a process that converts one or more substances into another substance.…

Q: In determination of microbial motility, what is the value of a hanging-drop perparation? what is the…

A: Hanging drop is a motility testing which helps microbiologist to view motility of bacterium.

Q: Salt Treatment Tap Water Treatment Distilled Water Treatment   Which of these experimental…

A: A hypertonic solution contains a higher concentration of solutes compared to another solution.…

Q: Agar is a solidifying agent used in media preparation.a. What is its origin? b. What makes it ideal…

A: A growth medium or culture medium is a gel or liquid designed to support microorganism growth. The…

Q: What is the substrate(s) that will be used by the microorganism to produce the calcite ?

A: Biomineralization is the process by which microorganisms and other living organisms directly or…

Q: Why is it necessary to store the molten agar in a water bath at 50 degrees Celsius?

A: Introduction:-  Agar is made up of two polysaccharides: agarose and agaropectin, with agarose…

Q: What type of agar is MacConkey agar?

A: MacConkey agar or MAC which is also described as a bacterial culture medium was named after the…

Q: Starch Hydrolysis: In many tests it is acceptable to read a positive result before the inoculation…

A: Starch hydrolysis test is used to identify a bacterial species. In this test, test bacteria are…

Q: operties of the Snyder deep media allow us to detect susceptibility to dental caries?

A: Dental caries (tooth decay) is injury to enamel of tooth that be converted into little holes or…

Q: If testing had come back as oxidase negative, urease positive and growth on MacConkey agar, what…

A: MacConkey agar is a selective and differential culture medium used for the isolation and…

Q: What is diluent? Why is it useful for working with bacterial samples?

A: Dilution is done to decrease the concentration of the original sample that is being tested. Dilution…

Q: What is the purpose of the different types of media (solid, semi-solid, broth)?

A: Culture medium is the medium consist of  mixture of nutrients and substances which are required for…

Q: Give the components, properties and special considerations in preparation (if there's any) on the…

A: MacConkey Agar . It is selective for gram- negative rods bacteria. EMB . It is selective for fecal…

Q: You are asked to make a 1:1000 dilution of a bacterial culture in a final volume of 5000 mL. What…

A: In this question, we are given with a solution of 1X concentration. We have to determine volume of…

Q: List 5 other methods of sterilization and describe the principle/s and when each method is used.

A: (Please note that, you provided more than one question, I can only answer one question at a time,…

Q: Agar is a solidifying agent used in media preparation. How and why does the agar concentration in…

A:

Q: Could a MacConkey agar plate be used in place of the EMB plate in the Standard Analysis of Water?…

A: MacConkey agar is used as a differential and selective culture medium used in analysis of water…

Q: What are the two components the medie must contain to test the bacteria for desulfurases

A: SIM (Sulfur, Iindole, Motility) medium differential medium is used mainly to test the ability of an…

Q: An unknown bacterium produces colorless colonies when inoculated onto an EMB plate. Predict what you…

A: EMB stands for Eosin Methylene Blue. It is a selective stain used for the growth of gram-negative…

Environmental health and hazards

An organism's environment is the environment in which it lives and has an impact on its well-being. The World Health Organization (WHO) defines environmental health as "those aspects of public health, such as quality of life, that are influenced by physical, chemical, biological, social, and psychosocial components in the environment." Environmental health is concerned with both natural and man-made settings in order to enhance public health. Environmental science, occupational medicine, toxicology, and epidemiology are the key subdisciplines of environmental health.