What is edman degradation and why does no reaction occur when sungsanpin was subjected to edman degradation show using structures.
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Question:
What is edman degradation and why does no reaction occur when sungsanpin was subjected to edman degradation show using structures.
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- REFLECT AND APPLY An amino acid mixture consisting of phenylalanine, glycine, and glutamic acid is to be separated by HPLC. The stationary phase is aqueous and the mobile phase is a solvent less polar than water. Which of these amino acids will move the fastest? Which one will move the slowest?REFLECT AND APPLY An amino acid mixture consisting of lysine, leucine, and glutamic acid is to be separated by ion-exchange chromatography, using a cation-exchange resin at pH 3.5, with the eluting buffer at the same pH. Which of these amino acids will be eluted from the column first? Will any other treatment be needed to elute one of these amino acids from the column?REFLECT AND APPLY Design an experiment to purify protein X on an anion-exchange column. Protein X has an isoelectric point of 7.0.
- REFLECT AND APPLY In reverse-phase HPLC, the stationary phase is nonpolar and the mobile phase is a polar solvent at neutral pH. Which of the three amino acids in Question 28 will move fastest on a reverse-phase HPLC column? Which one will move the slowest?REFLECT AND APPLY Sulfanilamide and related sulfa drugs were widely used to treat diseases of bacterial origin before penicillin and more advanced drugs were readily available. The inhibitory effect of sulfanilamide on bacterial growth can be reversed by p-aminobenzoate. Suggest a mode of action for sulfanilamide.REFLECT AND APPLY Albert Szent-Gyorgi, a pioneer in early photosynthesis research, stated, What drives life is a little electric current, kept up by the sunshine. What did he mean by this?
- REFLECT AND APPLY In the produce department of supermarkets, vegetables and fruits (cucumbers are an example) have been coated with wax for shipping and storage. Suggest a reason why this is done.REFLECT AND APPLY In lipid bilayers, there is an orderdisorder transition similar to the melting of a crystal. In a lipid bilayer in which most of the fatty acids are unsaturated, would you expect this transition to occur at a higher temperature, a lower temperature, or the same temperature as it would in a lipid bilayer in which most of the fatty acids are saturated? Why?REFLECT AND APPLY How does the presence of -bonds versus - bonds influence the digestibility of glucose polymers by humans? Hint: There are two effects.
- BIOCHEMICAL CONNECTIONS Beriberi is a disease caused by a deficiency of vitamin B1 (thiamine) in the diet. Thiamine is the precursor of thiamine pyrophosphate. In view of what you have learned in this chapter, why is it not surprising that alcoholics tend to develop this disease?MATHEMATICAL Determine the values of KM and Vmax for the decarboxylation of a -keto acid given the following data. substrateconcentration(molL1)Velocity(mMmin1)2.5001.0000.7140.5260.2500.5880.5000.4170.3700.256REFLECT AND APPLY Gel-filtration chromatography is a useful method for removing salts, such as ammonium sulfate, from protein solutions. Describe how such a separation is accomplished.