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1. What are the different factors that affects the quality of water?
2. What is the role of a coagulating agent in the process of purification?
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- 5. RX: Peppermint spirit 2ml Alcohol 10ml Sterile water QS 100ml Approximately how many fluid ounces of sterile water are needed to compound this solution?1. For each of the following separation methods covered in class, describe the basics of how that technique works, and what difference in material properties is used to separate molecules by that technique (for example: molecular size/MW, solubility differences, surface charge, ...) ultrafiltration, centrifugation, size-exclusion chromatography, isoelectric focusing. reverse osmosis, adsorption (physisorption), ion exchange, dialysis, affinity chromatography, electrophoresis andJoshua is showing a bacterium having high pH requirement for its growth. With this, he has to prepare a minimal medium at pH 9. Adding 0.5 ml of the pH indicator to 10 ml of the medium, the solution remained colorless. The pH of the medium was adjusted using 2.25 ml of the 0.05 N titrant. c.1. What was the pH indicator used by Joshua? c.2. What was the titrant he used?
- 1. give other urinary constituents which may reduce benedict’s qualitative solution other than glucose. 2. What Substances Give The Same Reaction As Sugar With Nylander’s Test And How Do You Remove It? 3. by what other method can reducing sugars be differentiated from each other and from glucose? 4. what substances interfere with the tests for fructose? for lactose? 5. what sugar causes the same color reactions with the test for pentoses? why? 6. what other substances interfere with the indican reactions? 7. Why should the specimen be albumin free when performing the chloride test for urine?1. How do you prepare 25 mL of a 0.4X solution of SDS from a 20X stock?2. How do you prepare 50 mL of a 0.25X solution of Trisethylenediaminetetraacetic acid (TE) buffer from a 100X stock?15. Calculate and explain how to make 800 mL of a 5% solution from a 20% solution. 16. Calculate and explain how to make 1500 mL of a 1:9 dilution from a 12M H3PO4 solution. 17. Calculate and explain how to make 800 mL of a 1/20 dilution from a 4M H3PO4 solution.
- 2nd Question: For the above conditions, what is the corresponding dosage of chlorine andmonochloramine to achieve the desired degree of disinfection. same effluent concentration (2 mg/L) for both free chlorine and monochloramine2. What are the substances added in the culture media utilized by the organism producing hydrogen sulfide? Give 2 other media used for the production of hydrogen sulfide. 3. Explain the mechanism taking place in the hydrolysis of urea which leads to the formation of bluish red color. 4. Discuss why human blood plasma will not always yield reliable results.Which of the following indicates buffering in the titration given in the image below? 12 11 10 Point 1 Point 2 8 7 pH Region 1 Region 2 6 4 3 Cocoo 2 + + + + + 10 15 20 25 30 35 40 45 50 ml of 0.1MHCI
- 1. Why is necessary to remove fat and tendons from the heart sample? 2. Why is necessary to completely grind the beef? 3. Why is necessary to balance the homogenate tubes for centrifugation? 4. How do you prepare a 100mL of 0.1 M phosphate buffer? 5. From the anterior buffer, how do you make 100mL of 0.05 M? 6. Calculate the amount of ammonium sulfate necessary to get a 20% solution? 7. Which is the importance of dialysis?3. What is the primary standard for iodine solutions? 4.QUESTION 3 An unknown protein was determined using the following method: Bradford method Reagents Stock Bradford reagent: Dissolve 100 mg Coomassie Brilliant Blue G250 in a mixture consisting of 100ml of 85% phosphoric acid, 50ml of 95% ethanol and 50ml 1M NaOH. Store at 4°C until precipitation occurs, at which point it is discarded. Working Bradford reagent: Prepare fresh by diluting 10ml of stock Bradford reagent to 250ml with distilled water. Stock bovine serum albumin (BSA) solution (10mg/ml): Dissolve 0.2g BSA in 20ml distilled water. Working BSA concentration range: 0.5 – 1.25 mg/ml Method 1. Prepare the following test tubes: Table 1: Preparation of test tubes for the Bradford method Blank Tube 1 Tube 2 Tube 3 Tube 4 Tube 5 Unknown Distilled H20 1.0 ml 0.9ml 0.9ml 0.9ml 0.9ml 0.9ml 0.8ml 0.25 mg/ml BSA 0.1 ml 0.5 mg/ml BSA 0.1 ml 0.75 mg/ml BSA 0.1 ml 1.0 mg/ml BSA 0.1 ml 1.25 mg/ml BSA 0.1ml Unknown 0.2ml protein Working 4.0ml 4.0ml 4.0ml 4.0ml 4.0ml 4.0ml 4.0ml Bradford 2.…