what are correct about enzyme kinetic parameters
Q: Which of the following is a type of irreversible enzyme inhibitor A) Mechanism-based (suicide)…
A:
Q: From a kinetics experiment, Kcat was determined to be 55sec^-1. For the kinetic assay, 0.05mL of a…
A: The concentration of enzyme stock solution was 0.05mg/ml. i.e. 1ml contain 0.05mg of enzyme. We took…
Q: A. Draw the chemical repeat unit of corn starch. B. What type of material is the corn starch/water…
A: Cornstarch consists of amylose and amylopectin. Both are polymers of glucose, but have different…
Q: Fructose 1,6-bisphosphatase (FBPase) is a key enzyme in gluconeogenesis. The mammalian enzyme is…
A: Gluconeogenesis is a process in which glucose is synthesize from non-carbohydrate sources like…
Q: The primary purpose of the aconitase step in Citric Acid Cycle is to: form the intermediate…
A: The citric acid cycle involves acetyl-CoA oxidation into carbon dioxide and water. The acetyl-CoA…
Q: ermination of translation occurs when the ribosome reaches one of _____ potential stop codons. At…
A: Introduction: The process of translation involves messenger RNA (mRNA) getting translated into…
Q: Which of these is not a reducing sugar
A: Carbohydrates are polyhydroxy aldehydes or ketones. They can be classified as monosaccharides,…
Q: Fluoroacetate is used as a poison for rat control. After entering a cell, fluoroacetate is converted…
A: The first step in the citric acid cycle is the formation of citric acid, by the condensation of…
Q: Bacteria and other microbes can be used to "clean up" an oil spill by breaking down oil into carbon…
A: In a double-stranded DNA, two complementary strands interact with each other by hydrogen bonds.…
Q: Orotic aciduria is treated with: ○ Tetrahydrofolate O 5-flourouricil O Uridine supplements O Calcium…
A: Patients suffering from Orotic aciduria have increased levels of orotic acid in their urine.…
Q: From the equation of a lineweaver-burke plot, calculate Km and Vmax. Show your work and include…
A: Enzymes are biochemical catalysts that are highly selective for a single reactant or substrate. Many…
Q: Glycogenesis occurs in both muscle and liver. Select one: O True O False Glycogen is released from…
A: Glycogen is storage-type homopolysaccharide that contain two types of glucose polymers: amylose:…
Q: Which nutrient has the highest thermic effect? O Glycogen Fat O Protein Carbohydrate
A: The rise in energy consumption brought on by consuming is known as the thermic effect of food (TEF).…
Q: H3C-(CH₂) OH H -C—C—C—SCOA HH OH H₂C—CH₂)_—C—C—C—SCOA H a. What kind of reaction is being performed…
A: The given molecule is a fatty acyl CoA. Fatty acids are great source of energy. These are oxidized…
Q: 4. Deducing quaternary structure via SDS-PAGE. SDS-PAGE is a convenient method for separating…
A: SDS-PAGE is a chromatographic technique that is used to separate proteins based on their molecular…
Q: The table below lists factors that regulate metabolite flow through the pyruvate dehydro- genase…
A: There are a wide variety of metabolic pathways that can take place within a cell. Hence , one…
Q: Provide the correct three-letter abbreviation for the following amino acid: H3N-CH-C- I CH3
A: The proteins are constituted of twenty naturally occurring amino acid that are connected by peptide…
Q: Some characteristics of three proteins are listed in the table below: Protein Molecular Weight (Da)…
A: Gel filtration chromatographic procedure in which the components are separated according to their…
Q: I. II. III. IV. O HẲN-CH-CANH-CH,C-NH-CH-CƠ V. O CH3 alanyl 2. Types of protein structures: What is…
A: "Since you have posted a question with multiple sub-parts, we will solve the first three sub-parts…
Q: discuss the excitatory NMDA receptor, the role of the Mg++ ion, and how NMDA activation can produce…
A: Hi! Thank you for the question. We are authorized to answer three subparts at a time, since you have…
Q: The substitution of His 64 of carbonic anhydrase II with Ala results in a sharp decrease in the…
A: Carbonic anhydrase II is the enzyme that catalyzes the formation of H2CO3 from CO2 and H2O, and…
Q: Question 14 The first three amino acids in a protein are lysine, glutamine, and serine. T describes…
A: The four classes of biological macromolecules are proteins, nucleic acids, carbohydrates and lipids.…
Q: Choose below the correct order of the oxygen binding proteins from lowest to highest affinity for…
A: Hemoglobin is a tetrameric oxygen transport protein found in erythrocytes, on the contrary,…
Q: What is the total number of moles of ATP generated per mole of glucose in the glycolytic pathway and…
A: Cellular respiration is a collection of three metabolic pathways that generate ATP by the oxidation…
Q: True or False: Spectrophotometric assays always track the rate of reaction through a. True b. False…
A: Spectrophotometry is the method that uses light beam to measure the concentration of a chemical or a…
Q: a) You evaluate the lipoxygenase inhibition by different concentrations of octyl protocathechuate.…
A: Inhibitors are the substances that bind to enzymes to regulate their activity . There are…
Q: C.Why does 500 mM imidazole result in the elution of a protein with a 6X-His tag?
A: Proteins are composed of amino acids, which are bound together by peptide linkage. Amino acids…
Q: Why is it important to create a restriction map/ vector map?
A: Genome mapping is a technique used to identify or map the locations of genetic markers which can be…
Q: HO -NH₂ NH NH HN S-CH3 ΝΗ O The peptide chain has OH residues. The name of the peptide using single…
A: Peptides are small sequences of amino acids that are joined together through peptide bonds. Peptides…
Q: v (micromol/sec) 120 100 80 60 40 20 0 <--X ...... Y 20 40 [A] (micromolar) 60 Enzyme X must have 2…
A: Assuming the enzyme follows Michaelis Mentos's enzyme kinetics. Km or Kd value is the substrate…
Q: The purpose of DNA gyrase in replication is: to relieve positive supercoiling induced by unwinding…
A: Introduction: The process of DNA replication involves the production of an exact copy of genetic…
Q: There are eight chemical reactions that occur in the citric acid cycle process. The reactions of…
A: In cellular respiration, glucose is oxidized during glycolysis and the end product (pyruvate) is…
Q: What is the sequence of the mRNA molecule synthesized from the DNA template strand…
A: The genetic information stored in DNA is copied to an mRNA through a process known as transcription.…
Q: Give an example of how Transamination takes place by a chemical reaction.
A: In transamination reaction, amino group is transferred from a donor substrate to an acceptor alpha…
Q: Consider the reaction: H H₂C-(CH₂)C=C—C—SCOA H ➤ H3C- 13C-(CH₂) What kind of reaction is being…
A: The given structure is a fatty acyl Coenzyme A. Fatty acids are charged with acetyl CoA which are…
Q: Draw the structure of a triacylglycerol containing stearic acid, palmitic acid, and oleic acid.
A: Animal fat and vegetable oil fall into the category of simple fats or triglycerides. Triglycerides…
Q: Using proper convention, provide the amino acid sequence for the following protein. O H₂N-CH- CH₂…
A: Proteins are large molecules made up of amino acid residues linked via a peptide bond. Amino Acids…
Q: Citrate synthase is inhibited by: NAD+ ADP NADH oxleoacetate All of the above None of the above
A: Explanation Glycolysis is a process by which glucose breaks into pyruvate and gives energy in the…
Q: Calculate the appropriate volume (in µL) of 9X loading buffer that should be added to 46.0 µL of a…
A: Agarose gel electrophoresis is a laboratory technique that is used for the separation of nucleic…
Q: Fill up the table below. For inferences, indicate the identity of the macronutrient being tested in…
A: Since you have posted a question with multiple sub-parts, we will solve first three sub-parts for…
Q: 1. Consider the reaction: succinyl-CoA succinate H3C H3C C H₂ a. What kind of reaction is being…
A: Acetyl CoA produced from fatty acid oxidation in the hepatic cells has two possible fates: enter…
Q: There are several ways to transform the Michaelis-Menten equation so as to plot data and derive…
A: For a one-substrate enzyme-catalyzed reaction, the Michaelis-Menton equation shows the quantitative…
Q: single-substrate reaction E+SESE+P.7 nditions: [S] > Km- ent with the condition that it describes.…
A: .
Q: Which of the following regarding the ATP Synthase is INCORRECT? Protons flow through the a and c…
A: ATP synthase, also called as Complex V is the protein complex responsible for synthesis of ATP from…
Q: Which major biosynthetic pathways utilize PRPP? de novo purine synthesis lysine biosynthesis…
A: INTRODUCTION: De novo purine synthesis- it is the synthesis of complex molecules from simple…
Q: A PCR reaction was performed to amplify the CHE2 gene. The PCR primers used were designed to amplify…
A: Polymerase chain reaction is a molecular biology technique that is sued to synthesize multiple…
Q: Identify what is asked Glycerol --> Glycogen Pyruvate --> Glucose Acetyl CoA --> Fats Fats-> Acetyl…
A: Metabolism is the total of all chemical transformation that takes place in a living cell. One…
Q: What is the biochemical cause and effect of anemia in sickle cell disease?
A: Sickle cell anemia is a genetic disease (it is an autosomal recessive disorder). This affects the…
Q: Which of the following correctly illustrates a dipeptide and an amino acid in the optimal position…
A: Proteins are composed of amino acids, which are bound together by peptide linkage. Amino acids…
Q: Please explain why hydrolysis of ATP can release much energy by describing how the products of ATP…
A: ATP hydrolysis is the catabolic reaction process in which chemical energy that is stored in the…
what are correct about enzyme kinetic parameters
Step by step
Solved in 2 steps
- Which of the following statements are false? Initial velocities of enzyme reactions are best obtained in the absence of product because it simplifies analysis. Initial velocities refer to the velocity of the reaction right after it is initiated. The velocity of the reaction as a function of measuring time are curved just like an isothermal binding curve because of substrate binding to the enzyme. Initial velocities correspond to the pre-steady state condition for free enzyme. Initial velocities can sometimes be measured by spectroscopy such as UV/Vis spectroscopy when monitoring the production of NADH from NAD+. The velocity of the reaction will eventually go to zero. The reaction will reach equilibrium because of the presence of the enzyme. It is always better to use substrate rather than product to measure enzyme kinetics.Which statements are false? Initial velocities of enzyme reactions are best obtained in the absence of product because it simplifies analysis. Initial velocities refer to the velocity of the reaction right after it is initiated. The velocity of the reaction as a function of measuring time are curved just like an isothermal binding curve because of substrate binding to the enzyme. Initial velocities correspond to the pre-steady state condition for free enzyme. Initial velocities can sometimes be measured by spectroscopy such as UV/Vis spectroscopy when monitoring the production of NADH from NAD+. The velocity of the reaction will eventually go to zero. The reaction will reach equilibrium because of the presence of the enzyme. It is always better to use substrate rather than product to measure enzyme kinetics.The figure below shows the dependence of the enzyme's rate, v (in µM/min), as a function of substrate concentration, S (in mM). Also shown is the dependence of the rate in the presence of an inhibitor, present at a concentration of 0.2 mM. Based on this information, which of the following does this inhibitor most likely interact with? 1/v 1- 05 1/[S) O A. Michaelis complex O B. [E O C. free enzyme O D. free substrate O E. Both "A" and "B."
- In an enzymatic reaction of maltase, the disaccharide maltose is hydrolyzed to glucose. In an experiment, an inhibitor X was added to the reaction. The addition of the inhibitor brought about a change in the activity of the enzyme. When the substrate concentration was 0.125 mM, the initial velocity was 0.10 mM per minute. However, Vo / Initial velocity changed to 0.25 mM per minute when the substrate concentration was 0.50 mM. Hence, calculate the of the reaction.In an enzymatic reaction of maltase, the disaccharide maltose is hydrolyzed to glucose. In an experiment, an inhibitor X was added to the reaction. The addition of the inhibitor brought about a change in the activity of the enzyme. When the substrate concentration was 0.125 mM, the initial velocity was 0.10 mM Vo per minute. However, / Initial velocity changed to 0.25 mM per minute when the substrate concentration was 0.50 mM. Hence, calculate the Km of the reaction.One way of expressing the rate at which an enzyme can catalyze a reaction is to state its turnover number. The turnover number is the maximum number of substrate molecules that can be acted on by one molecule of enzyme per unit of time. The turnover number for an enzyme (per second) is 752 How many molecules of substrate can one molecule of the enzyme act on in 19.2 min?
- Which of the following statements are true about enzyme regulation via noncovalent interactions? Select all that apply. Molecules can cause a change in enzyme shape because they're able to bind to the enzyme somewhere other than the active site The function of an enzyme is altered by a chemical change in its primary structure, which is called phosphorylation Molecules that are similar to the substrate in both size and shape can compete with the substrate for access to the enzyme's active site Molecules that are similar to the substrate in both size and shape can compete with the substrate for access to a location other than the active sitePlease note the reaction expression below. Which of the following rate constants describes the breakdown of the enzyme-substrate complex? There may be more than one answer. k₂ E+S OK-2 U k₂ 0 k₁ OK.1 k3 k.3 SES EPE+P K.3 K.₂ k.Select the graph that correctly illustrates the effect of a positive modifier (effector) on the velocity curve of an allosteric enzyme. Place the correct graph in the set of axes. The solid blue curve represents the unmodified enzyme. The dashed green curve represents the enzyme in the presence of the effector. R is the highly active form of the enzyme and T is the less active form of the enzyme. Assume that this is a positively cooperative enzyme, meaning that the affinity for substrate increases with increasing substrate concentration.
- An experiment on enzyme-catalyzed reaction was conducted in the laboratory by a student. Results obtained are summarized in the table below. In all the experiments, the concentration of the enzyme is the same. Substrate Concentration Velocity (pmol/min) (pmol) 1.5 0.21 3 0.28 4 0.32 0.36 8 0.4 15 0.45 18 0.47 1. Plot or graph these results using the Lineweaver-Burk method. 2. Determine the KM and Vmax values. Show all equations and calculations.The change in the amount of product B in a reaction which is catalyzed by Enzyme A is given in the table below. Determine the Vmax and Km of the enzyme in the absence and presence of C. Is C an inhibitor or an activator of enzyme A? Determine the type of inhibition or activation. Rate of formation of Product B in the presence of 25 mg/mL C (mM/min) Substrate Rate of formation of (mM) Product B (mM/min) 0.5 21.5 14.1 1 30.2 22.2 1.5 34.9 27.0 2.5 39.8 33.1 3.5 42.0 36.3An experiment on enzyme-catalyzed reaction was conducted in the laboratory by a student. Results obtained are summarized in the table below. In all the experiments, the concentration of the enzyme is the same. Substrate Concentration Velocity (pmol) (pmol/min) 1.5 0.21 0.28 4 0.32 6 0.36 0.4 15 0.45 18 0.47 1. Plot or graph these results using the Lineweaver-Burk method. 2. Determine the Km and Vmax values. Show all equations and calculations.