The restriction enzyme Xho I recognizes and cuts this sequence: 5-C/TCGAG-3. The restriction enzyme Sal I recognizes and cuts this sequence 5-G/TCGAG-3. In both cases, only one of the two DNA strands is shown, / indicates the cut site. A restriction fragment generated by digestion with Sal I is ligated into a plasmid cut at a unique Xho I site. After ligation, can the same fragment be cut back out of the plasmid, and if so, which enzyme(s) can do this? 1) Yes, using Xho1 but not Sall. 2) Yes, using Sal1 but not Xho1. 3) Yes, using either Sall or Xho1. 4) No, neither enzyme nor their combination will work. 5) Yes, using both enzymes - Sal 1 at one end and Xho 1 at the other end.
The restriction enzyme Xho I recognizes and cuts this sequence: 5-C/TCGAG-3. The restriction enzyme Sal I recognizes and cuts this sequence 5-G/TCGAG-3. In both cases, only one of the two DNA strands is shown, / indicates the cut site. A restriction fragment generated by digestion with Sal I is ligated into a plasmid cut at a unique Xho I site. After ligation, can the same fragment be cut back out of the plasmid, and if so, which enzyme(s) can do this? 1) Yes, using Xho1 but not Sall. 2) Yes, using Sal1 but not Xho1. 3) Yes, using either Sall or Xho1. 4) No, neither enzyme nor their combination will work. 5) Yes, using both enzymes - Sal 1 at one end and Xho 1 at the other end.
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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