The number of Microorganism A was exposed to a constant temperature of 117 C surviving spores of the microorganism was recorded throughout the exposure time. Calculate the D-value of the microorganism given the data below and briefly interpret the result. Time (mins.) No. of surviving spores 1.00 x 107 1.30 x 10 1.10 x 105 5 10 15 1.40 x 104 1.30 x 103 1.10 x 102 1.20 x 10' 20 25 30
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- The solution containing bacterial spores is heated in an autoclave. When the autoclave has reached a constant temperature of 121 °C, there are still 106 spores/ml. The specific death constant for bacterial spores at 121 ° C is 11.62 min-1. How long should the heating be continued at a constant temperature so that there are 1 spores/ml left? Enter the answer in minutes to three decimal places.D Question 37 Based on the data below, how much more UV resistant was B. licheniformis relative to S. aureus? (All controls had growth.) - covered with lid Organisms Exposure 20 sec Staphylococcus aureus 5 sec + 20 sec Bacillus licheniformis + 10 sec 80 sec times to UV light 40 sec 2.5 min 160sec 5 min 10 min 5 min 20 min 10 min 40 min 10 min + 40 minWith a plate count average of 297 and a final dilution on the plate of 1:1,000 ; what is the viable number of cells in original culture (CFU/ml)?
- You counted 4, 6, 12, 3 cells in each of the 4.outed squares of a hemacytomeyer. What are the cells per milliliter in that culture? If you resuspended your cell pellet 2.5 mL, what is the total cell count? How many uL do you need to add to a new culture if you want 4250 cells?Determine what percentage of the culture was living (viable) and what percentage was dead (mortality). Plates Plate Dilution Volume plated No.of colonies Avg No Concentration of diluted sample Cd(cells/mL) Concentration of original sample Cu(cells/mL) 1 10^-3 10ul R1=130,R2= 110,R3=210 150 150mL 1.50*10^6 Volume of cells(mL) Volume of diluent(mL) Total dilution(D) Hemocytometer count Avg cells in 1 mm^2 area Concentration of diluted sample Cd(cells/mL) Concentration of original sample Cu(cells/mL) 4.3 0.5 0.1 grid 1= 171 , grid2 = 185 178 1.78*10^5 1.78*10^6LAB SECTION I WAS PRESENT AND PERFORMED THIS EXERCISE (INITIALS) The Lethal Effect of Ultraviolet Radiation on Microbial Growth OBSERVATIONS AND INTERPRETATIONS Enter your class data below. Score the relative amount of growth on each plate. Use the numbers 0, 1, 2, or 3 (0 is no growth; 3 is abundant growth). Organism B. subtilis 5 seconds Imin 5+ 1 3 minutes 10 minutes 10 seconds 5+ 3min 30 seconds 5min 4+ minute 10min S. aureus 5 + 3 + 4 니- + 2 1 Micrococcus 1 10 sec 4 M 31m 10min 3'ın Questions of this exercise is to demonstrate the comparative effect of UV on two bacterial populations. The purpose This could have been accomplished without the cardboard cover. Why was the cover used? control site 1 Expe SECTION TWO MICROBIAL GROWTH DATA SHEET 2-1
- A 1.5-year-old child developed vomiting, diarrhea, and fever. Stool sample were inoculated into the Endo media. After 18 hours on the surface of the medium grew medium-sized, round, slightly convex red colonies with a metallic luster. The doctor suspected Escherichia coli. 1. Name the composition of the Endo agar media. 2. Describe the properties of bacterial colonies on the Endo media. 3. What purpose differential diagnostic Endo media used for?Uninoculated "controls" for each medium should score 0 (zero) in their respective tubes. Explain the importance of this affirmation 9, x10Provided with the following data, compute the corresponding CFU/ml of the original culture. ssume that spread plate technique was done. Show your solutions. 10-1 10-2 10-3 10-4 10-5 Plate 1 TNTC 340 132 45 19 Plate 2 TNTC TNTC 242 80 25 Plate 3 TNTC 280 90 22
- An otic product was subjected to AET and tested with the challenge microorganisms. An initial concentration of 2 x 10^5 cfu/mL of Escherichia coli was computed. Compute for the log reduction from the initial calculated count if the cfu/mL obtained is 1.2 x 10^4 at 7 days.A sterile powder vial label indicates to add 3.4 mL of sterile water which will produce a final volume of 4 mL. The increased volume is the result of: Select one: O a. O b. C. O d. The powder inside the vial was not lyophilized The type of diluent Displacement of the powder Incorrect calculation /mod/quiz/attempt.php?attempt=186101&cmid=102359&page=49# Q Search D L 贊助 8 9 Time left 22:22:18 HOAnswer each of the questions below using the plate images provided. Nutrient Agar 1 EMB Blood Agar 1. How many colonies are on NA plate 3?. 2. How many bacteria were transferred to NA plate 3?. 3. How many colonies are on NA plate 2? 4. How many bacteria were transferred to NA plate 2?. 5. How would you describe the growth on NA plate 1?. 6. How many colonies grew together on NA plate 1? 7. How many bacteria were transferred to NA plate 1? Nutrient Agar 2 8. How many bacteria collected from the source? (Hint: What fraction of tube 1 was spread on NA plate 1?) 9. Were any of the bacteria from the source Gram-negative? How can you tell?. 10. Could any of the bacteria from the source ferment lactose? How can you tell? Nutrient Agar 3 11. Were any of the bacteria from the source hemolytic? How can you tell?. 00