The hypothesis for the experiment shown in the picture is that the TamC3 and TamR3 cell lines function, and grow the absence of estrogen, receptor function. Reduced reliance on estrogen, receptor signaling alters mTOR pathway activity. If this hypothesis is true, then mediators of mTOR signaling (Akt or p70S6K) should have reduced levels of phosphorylation. Where would you look to see this? Why does the data in the picture not support the hypothesis?

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Cell Signaling

It allows cells to perform various cellular activities, coordinate, and interact with other cells. All cells of an organism receive signals from their environments and respond accordingly. Even the unicellular eukaryotes receive signals secreted by other cells and respond. In the case of yeast, mating takes place by signaling between the yeast cells. Communication by cell signaling in a multicellular organism has become a vast and important part of various fields of biology.