The health center cultured the throat swab and after doing a gram stain observed the picture to the right. The size bar measures 10mm with a ruler and the observed organisms have a diameter that measures 0.8 mm. What is the actual size of these organisms?
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- Which stain can be used to determine if bacteria are capsulated? Question options: flagella stain negative stain simple stain capsule stainWhat was the concentration of bacteria (CFU/mL) in the original suspensions used for dilutions and plating below: Dilution Amount plated # of colonies counted 10-4 1.0 mL 271 10-5 0.1 mL 36 10-6 1.0 mL 9While conducting the Hydrogen Sulfide Test (SIM Agar) you open up one of the tubes and it gives off a rotten egg smell while another tube you cultured does not. What does the smell indicate? How come there was no change in color in the tube that indicated a positive test? Please explain how the color change is to occur. Think critically.
- The first step in identifying an organism from a pure working culture is to perform a(n) __________ test. acid-fast staining negative staining endospore staining simple staining Gram stainThe unknown observations are shown as a mixed stain, gram positive, and gram negative. The next images shows the chart of what we can choose from for the gram positive bacterial stain. We are allowed to choose 4 test or stains that we would like to use to preform to identify the unknown.List the steps of the gram staining procedure in proper sequence and indicate the color of gram-positive and gram-negative bacteria after each step.
- Why should freshly collected samples be used for microscopic examination?While conducting the Hydrogen Sulfide Test you open up one of the tubes and it gives off a rotten egg smell while another tube you cultured does not. What does the smell indicate? How come there was no change in color in the tube that indicated a positive test? Explain how the color change is to occur. Think critically.An immersion lens was used to examine the microorganisms microscopically in a light microscope. ANSWER THE QUESTIONS 1. What symbols can be used to identify an immersion lens? What is the immersion system? 3. Why is it necessary to use an immersion lens to study microorganisms? 2.
- DESCRIBE THE BACTERIA OBSERVED. DO NOT DEFINE THE STAINING TECHNIQUES. PLEASE REFER TO THE PHOTO.A patient sample will be analyzed. You are responsible to quantify the bacteria number. The dilution series shown in the Figure ( Dilution.jpg) is prepared and 1 ml from each tube is plated. Plates were grown 24 hours resulting in colonies in each plate. Which plate you will use to quantify the bacteria in the patient sample? Why did you pick that plate? What is the dilution factor used in this series? Calculate the number of bacteria in the original patient sample. What would you do and why, if there were too many colonies to count on each plate?Sample diagram for the gram staining procedure and microscopic observation.