The distal Histidine (His 64) in myoglobin is subjected to three different mutations, this is one of them: H64N. (Histidine to Aparagine)   For the mutation, draw a theoretical binding curve and CO relative to the O2 and CO binding curves for wild-type Mb (see example below). Provide a clear rationale for the binding curve.

Q: When human hemoglobin undergoes a mutation, the mutant protein usually does not replace all of the…

A: The cytosol of red blood cells contains the oxygen-carrying globular protein hemoglobin, which is…

Q: If the following truncated peptide represents the active domain of the COVID19’s S-protein…

A: Mutations are alterations in the DNA sequence of an organism. Small changes, such as adding or…

Q: If this molecule can inhibit toxic effects at 0.5mM against gastric carcinoma cells, does it mean…

A: IC50 is the concentration at which 50% inhibitory effect is observed.

Q: What are the primary structures of TATA-binding protein (for ex. letter amino acids),  What are the…

A: TATA-binding proteins (TBP) bind to Sequences on DNA called TATA boxes. TBP is a general…

Q: Mutational analysis of the important amino acids in the catalytic triad of serine proteases showed…

A: The catalytic triad provides a paradigm for the structural and chemical features of enzymes that…

Q: You were recently tasked with the responsibility of generating an aptamer that was capable of…

A: In this modern Era of biotechnology, there are various molecular techniques which are of great…

Q: The first 32 amino acids from the N terminus of the protein bovine angiogenin were determined by…

A: Protein Sequence First of all we need to understand where chymotrypsin act, which bond (peptide…

Q: Would the following alterations to Src be oncogenic? Explain. (a) The mutation of Tyr 527 to Phe.…

A: Tyrosine kinases are an important subgroup of cellular receptors that play an important role in…

Q: A mutation in Hb that prevented formation of the tetramer would result in: a   A decrease in…

A: Hemoglobin are the iron containing  oxygen carrier. They are metalloprotein  that carries oxygen…

Q: 16a) The Iron Response Factor (IRF) is an RNA binding protein that recognizes and binds to a…

A: Iron response element or IRE refers to the stem-loop conserved structure that links to the iron…

Q: Based on what you know of scaffolding complexes, what would most likely happen if the Substrate…

A: Introduction Ubiquitin is a tiny regulatory protein with 76 amino acids that was identified in 1975.…

Q: A mutation that changes an alanine to a leucine in the nucleus of a protein leads to a less active…

A: Introduction: Each organism's DNA sequence is unique. Its base-pairs sequence can change from time…

Q: Approximate P50 for myoglobin from its binding curve. Approximate P50 for normal hemoglobin and the…

A: P50 is the oxygen tension or the pressure of oxygen at which hemoglobin or other oxygen binding…

Q: . Mutants of Neurospora crassa that lack carbamoyl phosphate syn- thetase I (CPS I) require arginine…

A: The process of carbamoyl phosphate synthetase I (CPS I) occurs in the mitochondria as the enzyme is…

Q: The alpha subunit of the heterotrimeric G-protein complex becomes activated upon activation of a…

A: Introduction: G Protein-coupled receptor (GPCR) also known as serpentine receptors or seven-pass…

Q: While each G-protein subunit has a distinctly characteristic C-terminal sequence that binds certain…

A: G-protein coupled receptors are heteromeric transmembrane proteins. GPCR is activated by specific…

Q: Mutants of Neurospora crassa that lack carbamoyl phosphate synthetase I (CPS I) require arginine in…

A: Carbamoyl Phosphate : It is an anion of biochemical significance. In land-dwelling animals, it is an…

Q: In trimetric G proteins the only function of the bamma y complex is to allow reactivating of the a…

A: G proteins are called the guanine nucleotide proteins that are responsible for switching on and off…

Q: . Suggest probable conseauences of the following real or possible hemoglobin mutations. (a) At B146…

A: Mechanism of protein mutation : H146 lies in the alpha/beta interface of the haemoglobin molecule.…

Q: What effect does binding of the IRF protein to the IRE in the mRNA encoding ferritin have on the…

A: Iron regulatory proteins(IRP) are previously known as IRF or IRE-BP. They plays a major role in iron…

Q: What is the dominant biochemical activity or molecular interaction that Sickle Cell Anemia…

A: Hemoglobin is an oligomeric conjugated protein with four peptide chains joined by a…

Q: In terms of binding adenylate kinase, the Ka for ATP is ~50 µM and the K, for GMP-PCP is ~50 nM.…

A: Here in the question, adenylate cyclase enzyme (ACE) substrate and inhibitor binding constant is…

Q: Once the chains of peptides that make up lysyl-tRNA synthetase protein are synthesized in ribosomes,…

A: Protein folding is the process where the proteins fold themselves to come to their native form. This…

Q: If the molecule can inhibit toxic effects at 0.5mM against gastric carcinoma cells, does it mean…

A: IC50 is the half inhibitory maximum concentration of a drug which inhibits the process 50%.

Q: Mutations in the Galactose-1-Phosphate Uridyl Transferase Gene Can you use RFLP to identify this…

A: Mutation --It is an alteration in the sequence of  nucleotides in a genome of an organism , virus…

Q: cytosol but those without this substance have concentrations of up to 50uM in the cytosol by the…

A: OmpF porin is a type of nonspecific protein that forms the pore at the outer membrane of bacteria…

Q: In a molecular disease of hemoglobin, Hemoglobin Rainier, Tyr 145β is replaced by Cys, which forms a…

A: Hemoglobin is a protein present in the red blood cell. The hemoglobin function is to transport the…

Q: Which of the following would be a good chemotherapy approach: blocking formation of the…

A: The cells require energy to divide, grow and multiply. The growth and division of cells are highly…

Q: Human histone H1 protein has the protein sequence as shown as “KKASKPKKAAS-…

A: Histone H1 protein K in the sequence represents lysine The given histone sequence is rich in lysine…

Q: If you were to remove the ER retrieval signal fromprotein disulfide isomerase (PDI), which is…

A: We got the information of first type in carboxyl terminus of soluble ER proteins and in golgi…

Q: Many neurodegenrative diseases are thought to be caused by increased levels of unfolded proteins.…

A: Neurodegenerative diseases are caused by the accumulation of misfolded proteins. Examples of…

Q: KIF1A has only a single motor domain. What additional structural elements would you expect to find…

A: KIF1A is kinesin like protein ,which act as the axonal transporter of synaptic vesicles or…

Q: A family of proteins known as cupredoxins contain a single redoxactive Cu ion coordinated by a Cys,…

A: Proteins are macromolecules or large biomolecules. Protein contains one or more long chains of amino…

Q: The folding and unfolding rate constants for a myoglobin mutant have been determined. The unfolding…

A: Protein folding is a biochemical process in which a random structure protein fold to attain a…

Q: 6.3.7 Activit (1) List and briefly explain four methods of studying an E-S complex. (2) (a) Which…

A: Amino Acids are organic compounds that combine to produce proteins, which is why they are known as…

Q: Bisphosphoglycerate lies in a central cavity within the hemoglobin tetramer, stabilizing the T…

A: Hemoglobin (Hb) is the protein complex, which is a tetramer in a structure that binds to the oxygen…

Q: Can you answer all the parts to this question please A. Where does a low barrier hydrogen bond…

A: Protease is an enzyme that is observed in both prokaryotes and eukaryotes and is involved in…

Q: GTP-y-s serves as an analog of GTP that cannot be hydrolyzed any further. How would a Co-IP…

A: co-IP or co- Immuno Precipitation is a technique used to study protein-protein interaction in-vivo.

Q: How is dynamic instability regulated by GTP hydrolysis? O GTP hydrolysis promotes addition of…

A: Dynamic instability is the condition, which is possible because of GTP hydrolysis. GTP distorted the…

Q: The mutation in hemoglobin at B82 Lys → Asp results in lowered O,-binding affinity compared to…

A: Hemoglobin : It is a protein in your red blood cells that carries oxygen to your body's organs and…

Q: Consider the now dominant variant of the SARS-CoV-2 called the D614G mutation: a) The mutation…

A: mRNA is translated to form protein. mRNAs contain trinucleotides sequences that code for particular…

Q: Below is a list of hypothetical Hemoglobin variants. Circle the name of the variant that is most…

A: Hemoglobin exists in the T and R state during the absence and presence of oxygen respectively. In…

Q: What type of regulation does the interaction of BPG with hemoglobin represent?

A: 2, 3-Bisphosphoglycerate (2,3-BPG) is most abundant organic phosphate in the red blood cell. Molar…

Q: As stated in the text, adenosine deaminase deficiency can be treated by injection of the stabilized…

A: Enzymes are defined as those substances that are used in a chemical process/reaction in order to…

Q: Which of the following would be a good chemotherapy approach: blocking formation of the…

A: Cancer is one of the most dreaded diseases of human beings and is a major cause of death all over…