You've got your Wooly Mammoth hemoglobin growing up like crazy in E. coli. You purify the protein and start running some assays, and realize that your protein is not functional. You suspect that this may be because protein folding into tertiary and quaternary structure is highly temperature sensitive. Your E. coli love to grow at 37C (same temperature as their human hosts!), which may be way too warm for Wooly Mammoth hemoglobin to fold properly. You decide to team up with Dr. Esaki of Kyoto University to test their low-temperature protein expression system using the cold-adapted bacterium, Shewanella sp. (DOI: 10.1128/AEM.00824-07). That means you have to start over with your cloning, but… That's okay; you're just stoked to be doing international collaborative research That's okay; you're just stoked to be doing genetics and molecular biology (always your favorite subjects since college and your awesome Genetics professor!) You're bummed; you didn't realize that that's why research is called re-search, because you often have to re-do and re-peat everything Both A and BWhat feature of DNA replication did Fred Sanger cleverly take advantage of when he invented dideoxy DNA sequencing using ddNTPs? DNA polymerase requires helicase to open the template strands. Wobble rules allow occasional mispairing of bases. RNA nucleotides are degraded faster because they lack a 2' OH. Ligase creates a phosphodiester bond. DNA polymerase can only add new nucleotides to a 3' OH.

Deoxy ribonucleic acid (DNA) is the genetic material of most organisms that carry coded genetic…

Answered: That's okay; you're just stoked to be…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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You've got your Wooly Mammoth hemoglobin growing up like crazy in E. coli. You purify the protein and start running some assays, and realize that your protein is not functional. You suspect that this may be because protein folding into tertiary and quaternary structure is highly temperature sensitive. Your E. coli love to grow at 37C (same temperature as their human hosts!), which may be way too warm for Wooly Mammoth hemoglobin to fold properly. You decide to team up with Dr. Esaki of Kyoto University to test their low-temperature protein expression system using the cold-adapted bacterium, Shewanella sp. (DOI: 10.1128/AEM.00824-07). That means you have to start over with your cloning, but…

		That's okay; you're just stoked to be doing international collaborative research
		That's okay; you're just stoked to be doing genetics and molecular biology (always your favorite subjects since college and your awesome Genetics professor!)
		You're bummed; you didn't realize that that's why research is called re-search, because you often have to re-do and re-peat everything
		Both A and B

What feature of DNA replication did Fred Sanger cleverly take advantage of when he invented dideoxy DNA sequencing using ddNTPs?

		DNA polymerase requires helicase to open the template strands.
		Wobble rules allow occasional mispairing of bases.
		RNA nucleotides are degraded faster because they lack a 2' OH.
		Ligase creates a phosphodiester bond.
		DNA polymerase can only add new nucleotides to a 3' OH.

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