TABLE 5.3 The sequence specificities of some proteolytic enzymes and cyanogen bromide R, O R2 O N-terminal -..-N- C-...C-teminal нн Enzyme Preferred Site Source R, = Lys, Arg From digestive systems of animals, many other sources Trypsin Chymotrypsin R, = Tyr, Trp, Phe, Leu Same as trypsin R, = Arg From blood; involved in coagulation Thrombin R, = Asp, Glu V-8 protease Prolyl endopeptidase Subtilisin From Staphylococcus aureus = Pro Lamb kidney, other tissues Very little specificity From various bacilli Carboxypeptidase A R2 = C-terminal amino acid From digestive systems of animals Thermolysin R2 = Leu, Val, Ile, Met From Bacillus thermoproteolyticus Cyanogen bromide R1 = Met
TABLE 5.3 The sequence specificities of some proteolytic enzymes and cyanogen bromide R, O R2 O N-terminal -..-N- C-...C-teminal нн Enzyme Preferred Site Source R, = Lys, Arg From digestive systems of animals, many other sources Trypsin Chymotrypsin R, = Tyr, Trp, Phe, Leu Same as trypsin R, = Arg From blood; involved in coagulation Thrombin R, = Asp, Glu V-8 protease Prolyl endopeptidase Subtilisin From Staphylococcus aureus = Pro Lamb kidney, other tissues Very little specificity From various bacilli Carboxypeptidase A R2 = C-terminal amino acid From digestive systems of animals Thermolysin R2 = Leu, Val, Ile, Met From Bacillus thermoproteolyticus Cyanogen bromide R1 = Met
Biochemistry
6th Edition
ISBN:9781305577206
Author:Reginald H. Garrett, Charles M. Grisham
Publisher:Reginald H. Garrett, Charles M. Grisham
Chapter18: Glycolysis
Section: Chapter Questions
Problem 25P: Using the ActiveModel for phosphofructokinase (Trypanosoma), describe the difference between the...
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What peptides are expected to be produced when α-melanotropin is cleaved by (a) trypsin, (b) cyanogen bromide, or (c) thermolysin? (as shown)
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