Selective media- which are these from the 3 we used? Differential media- which are these from the 3 we used? MSA and EMB are selective and differential, which for Gm- and which for Gm+? Be able to observe and interpret the results of growth and sugar fermentation (or not) looking at an MSA or EMB plate
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- Table 3 - Determination Tube # Potato Expt. Temp. 2c 3c 4c 5c extract (mL) 2 2 2 2 room temp 20 °℃ 40 °℃ 75 °℃ Boiling 100 °C of the optimum temperature of catechol oxidase enzyme. 1st Absorbance 0 min. at Expt. Temp. dH₂O Catechol (mL) (mL) 0 0 0 0 13 13 13 13 Start Time: 4:20 Absorbance: 0.072 Start Time: 4:25 Absorbance: 0.114 Start Time: 4:25 Absorbance: 2nd Absorbance after 10 min. in Expt Temp. Absorbance: 0.097 Time for reading: 4:30 Absorbance: 0.128 Time for reading: 4:33 Absorbance: Subtract 1st from 2nd absorbance 0.056 0.137 Time for reading: 4:35 Absorbance: 0.132 0-193 Start Time: 4:27 Time for reading: 437 Absorbance: 0-126 0.023 0.061 0.029 3rd Absorbance after 10 more min. at Room Temp. Time for reading: 4:40 Absorbance: Based on the data from Table 2 answer these questions: Q10) What is the enzyme's optimum temperature? Answer: Q11) Use the difference between 2nd and 1st absorbance to support your conclusion for the optimum temperature. 0.159 Time for reading: 4:43…Table 3-Determination Tube # Potato Expt. Temp. 2c 3c 4c 5c extract (mL) 2 2 2 2 room temp 20 °C 40 °C 75 °C of the optimum temperature of catechol oxidase enzyme. 1st Absorbance 0 min. at Expt. Temp. Boiling 100 °C dH₂O Catechol (mL) (mL) 0 0 0 0 13 13 13 13 Start Time: 4:20 Absorbance: 0.072 Start Time: 4:25 Absorbance: 0.114 Start Time: Absorbance: 25 0.132 Start Time: 4:27 Absorbance: 0.097 2nd Absorbance after 10 min. in Expt Temp. Time for reading: 4:30 Absorbance: 0.128 Time for reading: 4:33 Absorbance: 0.036 0-137 Time for reading: 4:35 Absorbance: Subtract 1st from 2nd absorbance 0.193 Time for reading: 4:37 Absorbance: 0-126 0.023 0.061 0.029 Based on the data from Table 2 answer these questions: Q10) What is the enzyme's optimum temperature? Answer: Q11) Use the difference between 2nd and 1st absorbance to support your conclusion for the optimum temperature 3rd Absorbance after 10 more min. at Room Temp. Time for reading: 4:40 Absorbance: 0.159 Time for reading: 4:43…Procedure 6: Respiration (Aerobic) 1. Obtain a small test tube of phenol red and carefully place six peas into the test tube with the dye. 2. Let the peas respire for 10-15 minutes. QUESTION 1. What color does the phenol red change to? Why?
- I am needing help with questions 1 through 3, please. 1) What is the importance of the Control Group in the Yeast and Sugar Balloon Experiment? 2) Write out the general summary equation for alcohol fermentation below using the formula for glucose; label the substrates and the products in this equation. 3) Research: Initial background about yeast and fermentation- write a few sentences about yeast and fermentation based on what you know. In what situations do humans use yeast for fermentation?Please answer them Asap for an upvote. Thank you. Explain the chemical process that causes the following reactions in the inoculated biochemical media: 1. In phenol fermentation tubes, gas generation and color changes are seen. 2. Changes in the color of the buffered glucose medium 3. TSI slant blackening of the mediumEXPERIMENT :UTILIZATION OF SOLID WASTE FOR PRODUCTION OF VALUE-ADDED PRODUCTS OBJECTIVES : To apply techniques/ technologies in waste management system for production of value-added products PROCEDURES: Separate the peels of different fruits were separated and oven dried at 50°C, ground and sieved through mesh screen. 50 ml of 10% (w/v) HCl was added to each waste (40 gm) in conical flask respectively. The mixture in water bath was placed at 100° C for one hour. The mixture was cooled down and the mixture was filtered through Whatman filter paper. The filtrate was diluted with sterile distilled water at varying concentration. The fruit hydrolysate was autoclaved at 121°C for 15 minutes. Saccharomyces cerevisiae was maintained on the YDPA at 28 °C. In 250 mL conical flasks, each of 100 mL of various fruit hydrolysate growth media supplemented was added with (NH4)2SO4 (2 gm), KH2PO4 (1gm), MgSO4.7H2O (0.5 gm), NaCl (0.1 gm), CaCl2 (0.1 gm). The initial pH of all the culture media was…
- Q. You have now carried out the Somogyi Nelson determination of reducing sugars and measured the absorption spectra of Tube 9. What is the optimum wavelength you should use to monitor the Somogyi Nelson Assay why? Table 1._SOMOGYI-NELSON DTERMENATION OF REDUCING SUGARS Tube 1 2 3 4 5 6 7 8 9 10 D-Glucose (160 µg/mL), mL 0 0 0.125 0.125 0.25 0.25 0.375 0.375 0.5 0.5 H2O, mL 0.5 0.5 0.375 0.375 0.25 0.25 0.125 0.125 0 0 D-Galactose (160 µg/mL), mL - - - - - - - - - - D-Arabinose (160 µg/mL), mL - - - - - - - - - - Glucose-Galactose Mixture, mL - - - - - - - - - - Absorbance (710 nm) 0.049 0.051 0.138 0.151 0.271 0.26 0.382 0.395 0.454 0.444 Absorbance - blank 0 0 0.088 0.101 0.221 0.21 0.332 0.345 0.404 0.394 Final [Reducing sugar]…»>> 33% 113 min 37 secs What are the applications of plant tissue culture? BIU Next = = = Only typed answer and also give a proper Explanation.1. Describe the role of sodium and phosphomolybdate reagent in Folin Wu method. 2. Describe the accuracy and specificity of Nelson-Somogyi method 3. Do you think PFF should be part of enzymatic method of glucose determination? Explain your answer.
- Home Tools 6weekwomensfull... ↓↓ Nelson Biology 11... 8.27 x 11.69 i 3 / 7 Unit 1- Quiz on Le... x 188% 7. Which of the follow is false with regards to Eubacteria? These bacteria can engage in either [or both] aerobic and anaerobic respiration O These bacteria form endospores to reproduce There are three types of these bacteria; thermophiles, methanogens, halophiles Both B and C are false Vimin that ramain daumont within the call munt ha in thin /thann svalafal 1 p Sign In (S C E E1. Given that the total magnification for the Anabaena photomicrograph above is 400X, and the magnification of the eubacterial cells in Exercise 1 (above) is 1000X, are these Anabaena larger or smaller than the eubacteria? Approximately how much larger/smaller are they? 2. What is the mechanism of action of Crystal Violet (i.e. what does it do to the cells and how does it do it)? Select an answer and submit. For keyboard navigation, use the up/down arrow keys to select an answer. a. CV turns only Gram positive cells purple by interacting with the cells' peptidoglycan. Gram negative cells do not change colour. b. CV turns all cells purple by interacting with the cells' peptidoglycan. c. CV turns all cells pink by interacting with the cells' peptidoglycan. d. CV turns all cells purple by interacting with the hydrophobic layers in the cell membrane. e. CV turns all cells pink by interacting with the hydrophobic layers in the cell membrane. 3. What is the…Topic: Yeast cells (dalmau plates) What are the principles of PDA, AcA, and MA plates in media preparation? please explain all