Select the hemoglobin variant(s) that are described by each statement. a) The variant least likely to cause pathological symptoms. b) The variant(s) likely to show pl values different from that of HbA on an isoelectric ocusing gel. c) The variant most likely to show a decrease in BPG binding and an increase in The overall affinity of the hemoglobin for oxygen. Answer Bank Hb Milwaukee Hb Cowtown Hb Memphis Hb Philly Hb Providence
Q: (5) Muscle and red blood cells produce lactate, which is then transported to the liver to support…
A: When glucose is available, oxygen is present and there is an energy demand; glucose is oxidised into…
Q: Calculate the standard free-energy change of the reaction catalyzed by the enzyme…
A: Standard free energy change () is the free energy change at standard conditions.Here;'R' is the…
Q: GQ5
A: The question is asking about the stage of cell division in which sister chromatids separate. Sister…
Q: 2. (6) Examine the two proteins labelled a) and b). Which of the two Ramachandran plots, c) or d) is…
A: Amino acids are backbone of proteins or polypeptide chains. When amino acids joined covalently…
Q: 1. Shown below is the intermediate in the aldolase mechanism, after dissociation of the first…
A: When drawing chemical reaction mechanisms, we use a curved arrow to show the flow of electrons. The…
Q: Fatty Acids-Draw out one fatty acid from the list given in class. Hydrocarbon chain atoms do not…
A: Fatty acids are carboxylic acids and structural components of lipids. Fatty acids are generally…
Q: Physiological Systems and Regulation Assessment 2: scenario based assessment Instructions This is…
A: The objective of the first question is to determine the optimal speed of travel for the treasure…
Q: Question 1 Listen Joey has the genotype Aa; Tt where A is the gene for albinism and T is the gene…
A: The question is asking us to determine the possible types of gametes that can be produced by an…
Q: Question 8 Listen In Labrador dogs, there are 2 genes that determine coat color. One gene has a…
A: The objective of this question is to determine the probability of a Labrador dog being black given…
Q: G Protein-Coupled Receptors
A: G Protein-Coupled Receptors (GPCRs) play a critical role in cellular signaling by transducing…
Q: Your tyrosinase enzyme assay results in a slope of 0.001946 sec-¹ for absorbance at 475 nm. Give the…
A: Before going into the calculation part, we need to know the principle behind the assay. The assay is…
Q: Which of the twenty naturally occurring amino acid side chains are charged at a pan of 1.00? pH of…
A: Amino acids can be defined as organic compounds which comprise of both amino & carboxylic acid…
Q: compound can be found with a tyrosyl unit?
A: Tyrosyl unit:This unit refers to a structural component which is derived from the amino acid…
Q: Dihybrid Cross Problem 9: Homozygous offspring of a dihybrid cross, again. In a dihybrid cross,…
A: The objective of this question is to determine the fraction of offspring that will be homozygous for…
Q: 3a.What is the state of the sunflower seed oil at room temperature? Solid or liquid? Explai why.…
A: Saturated and unsaturated fatsSaturated fats are solids at room temperature. As saturated fatty…
Q: Explain the underlying biomechanical constructs of spring, change of direction, and agility…
A: Spring-like biomechanical constructs refer to the ability of muscles and tendons to store and…
Q: 3. The IC50 value for a drug called razundib for a Src kinase is 15 nM. The Src kinase is known to…
A: Src kinase is a tyrosine kinase that catalyses the phosphate group transfer from ATP to the tyrosine…
Q: Question 2
A: The objective of the question is to understand the function of the metaphase checkpoint in the cell…
Q: 2.7 Now that you have figured out the reaction mechanism, let's explore the action mechanism of the…
A: Mechanism of the given reaction.Here the 2 chloroethylamine react with two nucleophilic groups of…
Q: Match the number in the image with the correct name for the lab supply 1 2 ♡ AGEN QIAGEN QIAGEN QIAC…
A: The industry standard for isolating total RNA is RNeasy Kits. They use 96 well plates or silicon…
Q: Lowest dissociation constant K B A B B Highest dissociation constant D C A
A:
Q: 5. Explain the difference between a centriole and a centromere
A: The objective of this question is to understand the difference between two key components of a cell:…
Q: Genetics Question 5
A: The question is asking us to determine the probability of a mouse offspring having a straight tail…
Q: What would the concentration of acetic acid be if 0.70 M CH3COO was adjusted to the pH of 5.76? (K₂…
A: The objective of the question is to calculate the concentration of acetic acid (CH3COOH) in a…
Q: 5. What is a saponification number? What does a high saponification number indicate? Name a lipid…
A: Biological macromolecules are the molecules that are required in sufficient quantity for the…
Q: Fill in the table below Monomeric units are called: What are unique monomeric units? Linkages…
A: Nucleic acids are biomolecules that are essential for all life forms. They are polymers of…
Q: Prepare a schematic diagram and present it as though it were a Figure in a publication (scientific…
A: Spiperone is an antagonist of D2 dopamine receptors. Because of this radiolabelled spiperone ( 3H…
Q: The enzyme dihydroneopterin aldolase (DHNA) is found in the metabolic pathway leading to folic acid.…
A: DHNA can catalyze two types of reactions;aldolase cleavage of DHNP to HP & GA andepimerization…
Q: a.Write the balanced reactions catalyzed by complex I, II, III, and 1V, and using these, write the…
A: The electron transport chain (ETC) involves the transfer of protons between the matrix (the negative…
Q: Consider the following scenario: A group of five treasure hunters have been hired to retrieve some…
A: The objective of the first part of the question is to determine the optimal speed of travel for the…
Q: Suppose a yeast enzyme performs this reaction: Glyceraldehyde 3 - phophospate (GAP or G3P) → 3- -…
A: Glycolysis is the metabolic pathway where glucose is catabolised into pyruvate with a net production…
Q: 2B. S. aureus hemolysin B attacks the RBC cell membrane by hydrolyzing the sphingomyelin headgroup:…
A: Sphingomyelin is a phospholipid which has phosphocholine as head group and sphingosine as platform…
Q: Each ionizable group of an amino acid can exist in one of two states, charged or neutral. The…
A: If the pKa is low, the pH of the solution is less, and the acidity of the solution is high. i.e.,…
Q: GQ7
A: The objective of the question is to identify the correct relationship between the two X chromosomes…
Q: Please fill in the four boxes bolded below you KM (MM) Vmax (nM/s) Type of inhibition…
A: Competitive inhibitors are the substances which are similar to substrate molecules in their…
Q: Determine whether each of the examples or phrases describes an essential amino acid, a nonessential…
A: Amino acids are biomolecules that have an amino group and a carboxyl group linked to the same carbon…
Q: Please draw out mechanism The formation of fructose 1, 6- biphophste from dihydroxyacetome phospahte…
A: Glycolysis:The metabolic process known as glycolysis, which in most organisms takes place in the…
Q: A different Fab fragment binds to lysozyme with a dissociation constant of Kd=10-6 M. A 1 nM (10-9…
A: Consider the following reaction: where P is the protein, L is the ligand and k1 and k2 are rate…
Q: Use excel to plot the following titration data. Once you have done your plot, make sure to label the…
A: pH = If [H+] concentration in a solution is high, the pH will be low and the solution will be…
Q: Structure Writing: Draw the structure of peptide with sequence G-A-N-D-A
A: If functional amine and carboxylic acid groups in amino acids join or combine together to form amide…
Q: The half-life of a drug in the bloodstream is 16 hours. By what factor does the concentration of the…
A: The half-life of a substance is the time it takes for half of the initial quantity of that substance…
Q: Question 5 Listen In cats, Short Hair (S) is dominant to long hair, Black fur (C) is dominant to…
A: The objective of this question is to determine the probability of a specific genetic outcome based…
Q: draw the atomic structure (include all H, C, O, and N) of the predominant ionization of lysine pHs…
A: Amino acids are biomolecules that have an amino group and a carboxyl group linked to the same carbon…
Q: if comparing proteins with a Tm=54 degrees C and one with a Tm =78 degrees c, which is more stable
A: The objective of the question is to determine which of the two proteins is more stable based on…
Q: A polypeptide was treated with 2-mercarptoethanol and produced the following fragments: Fragment 1:…
A: Mercapto ethanol is a chemical most commonly used in peptide sequencing to reduce the disulphide…
Q: (b) The GLUT system is highly selective for glucose. It also speeds up the rate of glucose transport…
A: The Glucode transporter (GLUT) is involved in the facilitated diffusion of glucose across the cell…
Q: Determine the fraction of Vmax that would be obtained when the substrate concentration, [S], is…
A: Michaelis-Menten equation:Leonor Michaelis and Maude Menten created a simple model that accounts for…
Q: The overall, net ionic charge on this peptide at pH = 7 would be: Cys-Ala-Glu-Arg-Met-Ser +2 -1 +1 O
A: Organic substances with both amino and carboxylic acid functional groups are called amino…
Q: What part of the cell is the molecule depicted below most likely to be located? cytoskeleton nuclear…
A: Lipids are a chemically diverse group of biomolecules that have two things in common:low solubility…
Q: Assume that an enzyme-catalyzed reaction follows the scheme shown: E + S ES E + P k₁ = 1 x 10%/M-s…
A: Michaelis Menten postulated that free enzyme reacts with the substrate reversibly to form an…
Trending now
This is a popular solution!
Step by step
Solved in 3 steps
- A person was found to have very low levels of functional beta globin mRNA and therefore very low levels of the beta globin protein. What problems would this cause for assembling functional haemoglobin molecules?Below is the O2 binding curve for adult Hb in whole blood (containing BPG) shown in red (labelled Hb). A Hb 20 40 60 80 100 pO2 (torr) a. Which curve is the best representation for stripped Hb (pure hemoglobin with heme, but no other small allosteric molecules associated)? [ Select ] b. Which curve has the lowest affinity (as measured by the p5o)? [ Select ] c. Which curve lacks cooperativity? [ Select ] Fraction of Hb bound with Oxygen 0.2 0.4 0.6 0.8 0'0 >Hemoglobin and myoglobin both use heme as their prosthetic group and they both bind O2. However, their O2-binding curves indicate these proteins have different activities. What lind of information can be learned about the differences between the curves?
- (b) The diagram on the right compares O2-dissoci- ation curves for human hemoglobins characterized by dif- ferent p50 values. If Ko2 represents the equilibrium disso- ciation constant for oxygen binding, e.g., Hb(02)n Hb + nO₂ show the relationship between Ko2 and p50. Assign one of the curves (a, b, e) that best approxi- mates the Ko2 of each of the mutant hemoglobins in the table above including HbA where Ko2 represents the equi- librium dissociation constant for oxygen binding. If no curve is present for any of the mutant hemogobins, state the rea- son why the mutant is not represented. Indicate on the graph where its p50 would lie. PERCENT SATURATION 100- 80- 60- 40- 20- PARTIAL PRESSURE OF OXYGEN lungs veins muscle 0- 0 20 bc/d T 100 120 140 40 60 80 OXYGEN PRESSURE (millimeters of mercury) (c) Of the mutant hemoglobins in the table above, which form a hydrogen bond stabilizing the R (oxy) conformation similarly to that in wild type HbA. Justify this statement on the basis of the…Figure 5 shows oxyhemoglobin dissociatlon curves at pH 7.2 and pH 7.4. Which of the following is true at PO2 less than 40 mm Hg? 27. % saturation af haemogiobin 100 pH 7.4 80 60 pH 7.2 40 + 20 10 20 30 40 50 60 70 Po; (mm Hg) Figure 5 A. Hemoglobin retains less O2 at pH 7.4 compared to pH 7.2. B. Hemoglobin retains less O2 at pH 7.2 compared to pH 7.4. C. The release of O2 from hemoglobin is not influenced by the production of CO2. D. At pH 7.2 where CO2 production is less than pH 7.4, hemoglobin releases less O2.Complete the following table about hemoglobin (Hb) characteristics. Оху-НЬ Deoxy-Hb Conformation [ Select ] [ Select ] ВPG [ Select ] [ Select ] > >
- Blood in our body changes color as a function of its oxygenation state. Given the spectral data below, calculate the absorbance of red light (600 nm) of fully oxygenated and deoxygenated blood. Using this data, determine what percentage of red light would go through a sample of blood that is 50% oxygenated. Assume the patient has a hemoglobin concentration of 10 mM and pathlength of 1 cm.Sketch out the binding site of Hemoglobin with oxygen bound . ( You can abstract the Heme ring out i.e. not draw each chemical bond). Please label both imported HIS residues.A 56-year-old man comes to the clinic complaining of persistent fatigue for the past three months. The physician decides to order multiple blood tests including an erythrocyte sedimentation rate and C-reactive protein. Which of the following best describes the clinical conclusion that should be reached if this patient's erythrocyte sedimentation rate is 22 mm/h and his C-reactive protein is 3.5 mg/dL? (A) Indicates the definitive presence of a rheumatoid disorder (B) Indicates the likely presence of infection or inflammation (C) Indicates the presence of a severe bacterial infection (D) Indicates the presence of cancer involving the bone marrow (E) Indicates the presence of rouleaux and the blood's ability to coagulate
- Below is a fractional saturation curve for O₂ binding to adult hemoglobin. Assume that curve Y represents a condition at pH 7.4 and with a normal physiological level of 2,3-BPG. Curve X represents a condition that Fractional saturation (0) 0.2 0.0 0 Tissue PO, (kPa) 12 Lungs1. The diagram on the right compares the O₂ binding prop- erties of normal adult hemoglobin (HbA) to those of one dubbed Hb Great-Lakes characterized by the mutation Leu(368) His. In the dia- gram Y = fraction of heme groups with bound O₂, and pO2, the par- tial pressure of O2 above the solution, is measured in units of torr or mmHg. Draw the corresponding Hill plot, being careful to reproduce graph- ically the dissociation equilibrium constant for O₂ binding (Ko2) for y each hemoglobin and to show the difference in the extent of allo- stery or cooperativity of subunit interaction in each type of hemo- globin. You need draw only the central, linear portion of the Hill plot. State what is the likely magnitude of the Hill constant (nH) for HbA from your reading and state what is the likely range of the magni- tude of the Hill coefficient for Hb Great Lakes. 1.0 0.8 0.6 0.4 0.2 Hb Great Lakes 0 10 20 Hb A 1 1 30 40 pO₂ (torr) I 50 60A patient carrying a mutant form of hemoglobin (KD = 48 torr) is planning to take part in a hiking trip that involves strenuous physical activity at ~10,000 feet above sea level. The mutant hemoglobin has reduced oxygen binding cooperativity (n = 2.2) and displays no major structural abnormalities. a. Calculate the percent saturation of hemoglobin in the lungs (pO2 = 70 torr at this elevation) for this patient and for an individual carrying a normal version of hemoglobin b. Calculate the percent saturation of hemoglobin in active skeletal muscle tissue (pO2 = 15 torr) for this patient and for an individual carrying a normal version of hemoglobin c. Will this patient transport oxygen from the lungs to active muscle tissues more or less efficientlythan an individual with a normal version of hemoglobin on this trip? Briefly explain your answer