Scatchard analysis rearranges the basic equation for protein-ligand binding to enable a linear fit of experimental data for bound to free ligand concentration ([L]bound/[L]free) as a function of bound ligand concentration ([L]bound). This enables extraction of the dissociation constant KD and the total protein concentration, [P]total, from the plot. Given the standard equation for a line, y = mx + b, where x is the abscissa and y is the ordinate, express KD in terms of the fitting parameters m and b.
Scatchard analysis rearranges the basic equation for protein-ligand binding to enable a linear fit of experimental data for bound to free ligand concentration ([L]bound/[L]free) as a function of bound ligand concentration ([L]bound). This enables extraction of the dissociation constant KD and the total protein concentration, [P]total, from the plot. Given the standard equation for a line, y = mx + b, where x is the abscissa and y is the ordinate, express KD in terms of the fitting parameters m and b.
Chapter4: Calculations Used In Analytical Chemistry
Section: Chapter Questions
Problem 4.2QAP
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a) Scatchard analysis rearranges the basic equation for protein-ligand binding to enable a linear fit of experimental data for bound to free ligand concentration ([L]bound/[L]free) as a function of bound ligand concentration ([L]bound). This enables extraction of the dissociation constant KD and the total protein concentration, [P]total, from the plot. Given the standard equation for a line, y = mx + b, where x is the abscissa and y is the ordinate, express KD in terms of the fitting parameters m and b.
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