PstI (5') CTGČAG (3') GACGTC EcoRI (5') GAÄTTC (3') CTTAAG

Basic Clinical Laboratory Techniques 6E
6th Edition
ISBN:9781133893943
Author:ESTRIDGE
Publisher:ESTRIDGE
Chapter1: The Clinical Laboratory
Section1.7: The Metric System
Problem 4RQ
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The partial sequence of one strand of a double-stranded DNA molecule is
5′ – – – GACGAAGTGCTGCAGAAAGTCCGCGTTATAGGCATGAATTCCTGAGG – – – 3′
The cleavage sites for the restriction enzymes EcoRI and PstI are shown below.
Write the sequence of both strands of the DNA fragment created when this DNA is cleaved with both EcoRI and PstI. The top strand of your duplex DNA fragment should be derived from the strand sequence given above

PstI
(5') CTGČAG (3')
GACGTC
EcoRI
(5') GAÄTTC (3')
CTTAAG
Transcribed Image Text:PstI (5') CTGČAG (3') GACGTC EcoRI (5') GAÄTTC (3') CTTAAG
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