Homework Help is Here – Start Your Trial Now!
Science
Chemistry
Procedure: 1. Heat denaturation - heat 2 ml of 2% albumin solution to boiling ina test tube. Add a pinch of NaCl. Observe theresult. 2. Denaturation by Acid - to 3ml albumin, add 1 ml of 1M sulfuric acid. Allow to stand for 10 minutes. Add 1 drops of methyl orange indicator. Note the color of solution. (methyl red is color red at pH below 5.3; yellow at pH above 5.3 and orange at near pH 5.3). Neutralize with 1M NaOH drop by drop stirrring after every drop until an orange color is obtained. 3. Alkali Denaturation - to 3 ml albumin solution, add 1 ml of 1M NaOH. Allow to stand for 10 minutes. Add 1 drops of methyl orange indicator. Note the color of the solution. Neutralize with 1M sulfuric acid drop by drop until a pink color is obtained. 4. Denaturation by Organic Solvents - add 3 ml of 95% ethyl alcohol to 3 ml albumin solution (which must contain some inorganic salts such as NaCl) and filter. Test the solubility of precipitated protein in water. 5. Precipitation by Metallic Salts - to 2 ml albumin, add 5% HgCl₂ drop by drop until no precipitate is formed. Note color of precipitate formed. Repeat using 2% CuSO4 2% FeCl3, 1% AgNO3, 1% lead acetate instead of HgCl₂. 6. Precipitation by Alkaloidal Reagent - to 2 ml albumin, add picric acid drop by drop until no precipitate is formed. Note the color of the precipitate. Repeat using 10% TCA and tannic acid instead of picric acid. 7. Precipitation by Strong Acids - place 1 ml of conc. HNO, in a test tube. Incline the tube and pour little by little albumin solution along the side of the tube to form a layer. Note what is produced at the zone between the two liquids.

Procedure: 1. Heat denaturation - heat 2 ml of 2% albumin solution to boiling ina test tube. Add a pinch of NaCl. Observe theresult. 2. Denaturation by Acid - to 3ml albumin, add 1 ml of 1M sulfuric acid. Allow to stand for 10 minutes. Add 1 drops of methyl orange indicator. Note the color of solution. (methyl red is color red at pH below 5.3; yellow at pH above 5.3 and orange at near pH 5.3). Neutralize with 1M NaOH drop by drop stirrring after every drop until an orange color is obtained. 3. Alkali Denaturation - to 3 ml albumin solution, add 1 ml of 1M NaOH. Allow to stand for 10 minutes. Add 1 drops of methyl orange indicator. Note the color of the solution. Neutralize with 1M sulfuric acid drop by drop until a pink color is obtained. 4. Denaturation by Organic Solvents - add 3 ml of 95% ethyl alcohol to 3 ml albumin solution (which must contain some inorganic salts such as NaCl) and filter. Test the solubility of precipitated protein in water. 5. Precipitation by Metallic Salts - to 2 ml albumin, add 5% HgCl₂ drop by drop until no precipitate is formed. Note color of precipitate formed. Repeat using 2% CuSO4 2% FeCl3, 1% AgNO3, 1% lead acetate instead of HgCl₂. 6. Precipitation by Alkaloidal Reagent - to 2 ml albumin, add picric acid drop by drop until no precipitate is formed. Note the color of the precipitate. Repeat using 10% TCA and tannic acid instead of picric acid. 7. Precipitation by Strong Acids - place 1 ml of conc. HNO, in a test tube. Incline the tube and pour little by little albumin solution along the side of the tube to form a layer. Note what is produced at the zone between the two liquids.

Chemistry
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
See similar textbooks
icon
Related questions
Question
results test HgCl, Color of ppt 5. Metallic Salts CUSO, Color of ppt FeCl, Color of ppt AGNO, Color of ppt Pb(Ac): Color of ppt 6. Alkalloidal Picric Acid Reagent Color of ppt. TCA Color of ppt Tannic Acid Color of ppt 7. Conc. HNO, Strong Acid
Transcribed Image Text:results test HgCl, Color of ppt 5. Metallic Salts CUSO, Color of ppt FeCl, Color of ppt AGNO, Color of ppt Pb(Ac): Color of ppt 6. Alkalloidal Picric Acid Reagent Color of ppt. TCA Color of ppt Tannic Acid Color of ppt 7. Conc. HNO, Strong Acid
Procedure: 1. Heat denaturation – heat 2 ml of 2% albumin solution to boiling ina test tube. Add a pinch of NaCl. Observe theresult. 2. Denaturation by Acid – to 3ml albumin, add 1 ml of 1M sulfuric acid. Allow to stand for 10 minutes. Add 1 drops of methyl orange indicator. Note the color of solution. (methyl red is color red at pH below 5.3; yellow at pH above 5.3 and orange at near pH 5.3). Neutralize with 1M NaOH drop by drop stirrring after every drop until an orange color is obtained. 3. Alkali Denaturation – to 3 ml albumin solution, add 1 ml of 1M NaOH. Allow to stand for 10 minutes. Add 1 drops of methyl orange indicator. Note the color of the solution. Neutralize with 1M sulfuric acid drop by drop until a pink color is obtained. 4. Denaturation by Organic Solvents – add 3 ml of 95% ethyl alcohol to 3 ml albumin solution (which must contain some inorganic salts such as NaCl) and filter. Test the solubility of precipitated protein in water. 5. Precipitation by Metallic Salts – to 2 ml albumin, add 5% HgCl, drop by drop until no precipitate is formed. Note color of precipitate formed. Repeat using 2% CUSO, 2% FeCl,, 1% AgNO,, 1% lead acetate instead of HgCl,. 6. Precipitation by Alkaloidal Reagent – to 2 ml albumin, add picric acid drop by drop until no precipitate is formed. Note the color of the precipitate. Repeat using 10% TCA and tannic acid instead of picric acid. 7. Precipitation by Strong Acids – place 1 ml of conc. HNO; in a test tube. Incline the tube and pour little by little albumin solution along the side of the tube to form a layer. Note what is produced at the zone between the two liquids.
Transcribed Image Text:Procedure: 1. Heat denaturation – heat 2 ml of 2% albumin solution to boiling ina test tube. Add a pinch of NaCl. Observe theresult. 2. Denaturation by Acid – to 3ml albumin, add 1 ml of 1M sulfuric acid. Allow to stand for 10 minutes. Add 1 drops of methyl orange indicator. Note the color of solution. (methyl red is color red at pH below 5.3; yellow at pH above 5.3 and orange at near pH 5.3). Neutralize with 1M NaOH drop by drop stirrring after every drop until an orange color is obtained. 3. Alkali Denaturation – to 3 ml albumin solution, add 1 ml of 1M NaOH. Allow to stand for 10 minutes. Add 1 drops of methyl orange indicator. Note the color of the solution. Neutralize with 1M sulfuric acid drop by drop until a pink color is obtained. 4. Denaturation by Organic Solvents – add 3 ml of 95% ethyl alcohol to 3 ml albumin solution (which must contain some inorganic salts such as NaCl) and filter. Test the solubility of precipitated protein in water. 5. Precipitation by Metallic Salts – to 2 ml albumin, add 5% HgCl, drop by drop until no precipitate is formed. Note color of precipitate formed. Repeat using 2% CUSO, 2% FeCl,, 1% AgNO,, 1% lead acetate instead of HgCl,. 6. Precipitation by Alkaloidal Reagent – to 2 ml albumin, add picric acid drop by drop until no precipitate is formed. Note the color of the precipitate. Repeat using 10% TCA and tannic acid instead of picric acid. 7. Precipitation by Strong Acids – place 1 ml of conc. HNO; in a test tube. Incline the tube and pour little by little albumin solution along the side of the tube to form a layer. Note what is produced at the zone between the two liquids.
Expert Solution
steps

Step by step

Solved in 3 steps

SEE SOLUTIONCheck out a sample Q&A here
Blurred answer
Knowledge Booster
Analytical Separations
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, chemistry and related others by exploring similar questions and additional content below.
Similar questions
  • SEE MORE QUESTIONS
Recommended textbooks for you
Chemistry
Chemistry
Chemistry
ISBN:
9781305957404
Author:
Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:
Cengage Learning
Chemistry
Chemistry
Chemistry
ISBN:
9781259911156
Author:
Raymond Chang Dr., Jason Overby Professor
Publisher:
McGraw-Hill Education
Principles of Instrumental Analysis
Principles of Instrumental Analysis
Chemistry
ISBN:
9781305577213
Author:
Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Publisher:
Cengage Learning
Organic Chemistry
Organic Chemistry
Chemistry
ISBN:
9780078021558
Author:
Janice Gorzynski Smith Dr.
Publisher:
McGraw-Hill Education
Chemistry: Principles and Reactions
Chemistry: Principles and Reactions
Chemistry
ISBN:
9781305079373
Author:
William L. Masterton, Cecile N. Hurley
Publisher:
Cengage Learning
Elementary Principles of Chemical Processes, Bind…
Elementary Principles of Chemical Processes, Bind…
Chemistry
ISBN:
9781118431221
Author:
Richard M. Felder, Ronald W. Rousseau, Lisa G. Bullard
Publisher:
WILEY
SEE MORE TEXTBOOKS