Prior to subculture, Tifa used a hemocytometer to count her HepG2 cells cultured on a T-25flask. After trypsinization, she prepared her cells by mixing 50 µL of the cell suspension with50 μL of 0.4% trypan blue. Her observation under the microscope is as shown below:1(1)(ii)3(iii)2(iv)4Note: Count cells on the four labelled squares. Include cells touching the line on top and left.18P06.¿66ⓇDetermine the number of viable and dead cells from her observation.What is the percentage of viability of this culture? Show your calculations in detail.Calculate the concentration of viable cells per mL in the original culture. Show yourcalculations in detail.Based on your understanding of cell culture, do you think she should proceed withsubculture? Justify your answer.

In the field of cell culture, accurately assessing the viability and concentration of cells is…

Prior to subculture, Tifa used a hemocytometer to count her HepG2 cells cultured on a T-25 flask. After trypsinization, she prepared her cells by mixing 50 μL of the cell suspension with 50 μL of 0.4% trypan blue. Her observation under the microscope is as shown below: 3 (i) (ii) (iii) 2 (iv) 4 o 0 1000 466 © Ø Note: Count cells on the four labelled squares. Include cells touching the line on top and left. O Oe Determine the number of viable and dead cells from her observation. What is the percentage of viability of this culture? Show your calculations in detail. Calculate the concentration of viable cells per mL in the original culture. Show your calculations in detail. Based on your understanding of cell culture, do you think she should proceed with subculture? Justify your answer.

Prior to subculture, Tifa used a hemocytometer to count her HepG2 cells cultured on a T-25 flask. After trypsinization, she prepared her cells by mixing 50 μL of the cell suspension with 50 μL of 0.4% trypan blue. Her observation under the microscope is as shown below: 3 (i) (ii) (iii) 2 (iv) 4 o 0 1000 466 © Ø Note: Count cells on the four labelled squares. Include cells touching the line on top and left. O Oe Determine the number of viable and dead cells from her observation. What is the percentage of viability of this culture? Show your calculations in detail. Calculate the concentration of viable cells per mL in the original culture. Show your calculations in detail. Based on your understanding of cell culture, do you think she should proceed with subculture? Justify your answer.

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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A culture of Staphylococcus is diluted as follows:(1) 20mL are added to 80mL of water.(2) 10uL from (1) are then added to 9.99mL of water.(3) A 10-2 dilution is made from tube # (2).(4) 100uL from (3) are plated for a pour plate and incubated. There were 34 colonies counted on one quarter of the plate following incubation. a) What was the overall dilution?b) How many cfu/mL were present in the original culture?
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N Please observe the following cells that have been Gram stained. "* 3 E ER $ JO 4 R % 5 6 F G & Y I U 8 00