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Primer pairs of homo sapiens isolate breast cancer brca1 gene. Select the best primer pair then give 5 reasons why is it the best

Primer pairs of homo sapiens isolate breast cancer brca1 gene. Select the best primer pair then give 5 reasons why is it the best

An Illustrated Guide To Vet Med Term
An Illustrated Guide To Vet Med Term
4th Edition
ISBN:9781305465763
Author:ROMICH
Publisher:ROMICH
Chapter2: Where, Why, And What
Section: Chapter Questions
Problem 1M
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Primer pairs of homo sapiens isolate breast cancer brca1 gene. Select the best primer pair then give 5 reasons why is it the best
Primer pair 1 Forward primer Reverse primer Product length Primer pair 2 Forward primer Reverse primer Product length Primer pair 3 Forward primer Reverse primer Product length Primer pair 4 Forward primer Reverse primer Product length Primer pair 5 Forward primer Reverse primer Product length Primer pair 6 Forward primer Reverse primer Product length Sequence (5'->3') ACATGCCCACAGGTAAGAGC GCAATCTGAGGAACCCCCAT 111 Sequence (5'->3¹) ATGGGGGTTCCTCAGATTGC TGCTCTTGAGAAGGGGGACA 131 Sequence (5'->3') GGTAAGAGCCTGGGAGAACC AACCCCCATCGTGGGATCTT 89 Sequence (5'->3') CTTCACCAACATGCCCACAG TTCAGCAATCTGAGGAACCCC 123 Sequence (5'->3') GGGGGCTAGAAATCTGTTGCT CTGGAACTCTGGGGTTCTCC 79 Sequence (5'->3') GGGAGAACCCCAGAGTTCCA ACCCCCATCGTGGGATCTT 77 Template strand Plus Minus Template strand Plus Minus Template strand Plus Minus Template strand Plus Minus Template strand Plus Minus Template strand Plus Minus Length Start Stop Tm 20 20 Length 20 20 Length 20 20 Length 20 21 Length 20 19 GC% 250 269 60.04 55.00 4.00 360 341 59.74 55.00 3.00 Self complementarity Start Stop Tm GC% Self complementarity 341 360 59.74 55.00 3.00 471 452 60.47 55.00 4.00 Start Stop Tm GC% 261 280 59.46 60.00 349 330 60.92 55.00 Start Stop Tm GC% 242 261 59.40 55.00 364 344 60.00 52.38 Self complementarity 3.00 6.00 Self complementarity 4.00 5.00 Length Start Stop Tm GC% 21 214 234 60.06 52.38 4.00 292 273 59.38 60.00 6.00 20 Self complementarity Start Stop Tm GC% Self complementarity 272 291 60.84 60.00 6.00 348 330 60.31 57.89 6.00 Self 3' complementarity 2.00 2.00 Self 3' complementarity 2.00 0.00 Self 3' complementarity 0.00 2.00 Self 3' complementarity 1.00 2.00 Self 3' complementarity 3.00 2.00 Self 3' complementarity 2.00 0.00
Transcribed Image Text:Primer pair 1 Forward primer Reverse primer Product length Primer pair 2 Forward primer Reverse primer Product length Primer pair 3 Forward primer Reverse primer Product length Primer pair 4 Forward primer Reverse primer Product length Primer pair 5 Forward primer Reverse primer Product length Primer pair 6 Forward primer Reverse primer Product length Sequence (5'->3') ACATGCCCACAGGTAAGAGC GCAATCTGAGGAACCCCCAT 111 Sequence (5'->3¹) ATGGGGGTTCCTCAGATTGC TGCTCTTGAGAAGGGGGACA 131 Sequence (5'->3') GGTAAGAGCCTGGGAGAACC AACCCCCATCGTGGGATCTT 89 Sequence (5'->3') CTTCACCAACATGCCCACAG TTCAGCAATCTGAGGAACCCC 123 Sequence (5'->3') GGGGGCTAGAAATCTGTTGCT CTGGAACTCTGGGGTTCTCC 79 Sequence (5'->3') GGGAGAACCCCAGAGTTCCA ACCCCCATCGTGGGATCTT 77 Template strand Plus Minus Template strand Plus Minus Template strand Plus Minus Template strand Plus Minus Template strand Plus Minus Template strand Plus Minus Length Start Stop Tm 20 20 Length 20 20 Length 20 20 Length 20 21 Length 20 19 GC% 250 269 60.04 55.00 4.00 360 341 59.74 55.00 3.00 Self complementarity Start Stop Tm GC% Self complementarity 341 360 59.74 55.00 3.00 471 452 60.47 55.00 4.00 Start Stop Tm GC% 261 280 59.46 60.00 349 330 60.92 55.00 Start Stop Tm GC% 242 261 59.40 55.00 364 344 60.00 52.38 Self complementarity 3.00 6.00 Self complementarity 4.00 5.00 Length Start Stop Tm GC% 21 214 234 60.06 52.38 4.00 292 273 59.38 60.00 6.00 20 Self complementarity Start Stop Tm GC% Self complementarity 272 291 60.84 60.00 6.00 348 330 60.31 57.89 6.00 Self 3' complementarity 2.00 2.00 Self 3' complementarity 2.00 0.00 Self 3' complementarity 0.00 2.00 Self 3' complementarity 1.00 2.00 Self 3' complementarity 3.00 2.00 Self 3' complementarity 2.00 0.00
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