Chemistry & Chemical Reactivity
10th Edition
ISBN:9781337399074
Author:John C. Kotz, Paul M. Treichel, John Townsend, David Treichel
Publisher:John C. Kotz, Paul M. Treichel, John Townsend, David Treichel
Chapter14: Chemical Kinetics: The Rates Of Chemical Reactions
Section14.7: Reaction Mechanisms
Problem 1.1ACP
Related questions
Question
Chemistry
Please estimate the Km and Vmax and then calculate the kinetic constants (Km, Vmax, kcat, and kcat/Km) Please and thank you <3
![2. Aldolase
Aldolase is a 157 kD enzyme that catalyzes the following reaction:
Aldolase
OH
HO
OH
D-Fructose-1,6-diphosphate
2.5
5
10
25
50
1
[s] (uM) Vo (uM/min)
1
3.9
8.1
15.3
22.0
28.0
33.0
rofalan
Dihydroxyacetone phosphate
D-Glyceraldehyde-3-phosphate
In an assay of Aldolase (100 µL volume), the production of D-Glyceraldehyde-3-phosphate (GAP) is detected
by modification with excess hydrazine to give a hydrazone that absorbs at 240 nm (624028500 M¹cm¹¹). The
enzyme concentration for the assay is 150 pM, and the following data is obtained:
OH
H₂NNH₂
OH](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Fd796ae82-b7a1-4aac-bdc2-618bcc17e432%2F8aa0699d-0b7b-49d9-9b73-8baedc3fedc8%2F7r8z1z_processed.jpeg&w=3840&q=75)
Transcribed Image Text:2. Aldolase
Aldolase is a 157 kD enzyme that catalyzes the following reaction:
Aldolase
OH
HO
OH
D-Fructose-1,6-diphosphate
2.5
5
10
25
50
1
[s] (uM) Vo (uM/min)
1
3.9
8.1
15.3
22.0
28.0
33.0
rofalan
Dihydroxyacetone phosphate
D-Glyceraldehyde-3-phosphate
In an assay of Aldolase (100 µL volume), the production of D-Glyceraldehyde-3-phosphate (GAP) is detected
by modification with excess hydrazine to give a hydrazone that absorbs at 240 nm (624028500 M¹cm¹¹). The
enzyme concentration for the assay is 150 pM, and the following data is obtained:
OH
H₂NNH₂
OH
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