PCR-based assay was used for the detection of Candida albicans, please help me answer the following questions so I can use them as a guide. What are the advantages and disadvantages of performing Molecular-based Assays over the routine diagnostic tests for the Candida albican identification? What is the basis for the nucleic acid extraction that you utilized in this assay? What is the basis for the choice of primers that you utilized in this assay? Elaborate.

Basic Clinical Laboratory Techniques 6E
6th Edition
ISBN:9781133893943
Author:ESTRIDGE
Publisher:ESTRIDGE
Chapter2: Basic Hematology
Section2.3: Microhematocrit
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PCR-based assay was used for the detection of Candida albicans, please help me answer the following questions so I can use them as a guide.

What are the advantages and disadvantages of performing Molecular-based Assays over the routine diagnostic tests for the Candida albican identification?

What is the basis for the nucleic acid extraction that you utilized in this assay?

What is the basis for the choice of primers that you utilized in this assay? Elaborate.

What are the potential sources of errors in the methods that you have utilized?

 

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