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Of the 3 techniques that you used in this lab (direct fluorescent antibody, ouchterlony diffusion, ELISA) which do you think would work best for blood typing (A, B, O) and why?
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- Labs that do a lot of Western blots often have hundreds of primary bodies but only two or three secondary antibodies. Why? I understand that secondary antibody aids in the detection, sorting or purification of target antigens by binding to the primary antibody which directly binds to the target antigen. However, I don't undrstand why there are only two or three secondary antibodies available in the laboratory. Glad if the expert would advise.A technologist failed to notice that the centrifuge had not properly centrifuged the test tubes prepared for antibody identification. The time of centrifugation was 15 seconds instead of 30 seconds. What would be the potential error in the interpretation of this test? can please any one provide me ans?If you were using the ELISA to look for the presence of antibodies and the sample gave a negative result, does this mean that there were no antibodies present? Explain.
- Which of the following media is capable of detecting all clinically significant antibodies while avoiding all clinically insignificant antibodies? Question 1 options: Gel technology Tube technique with PEG No single media is capable of doing this Solid phaseYou just received the properly labeled blood bank specimen on patient Aran Stark. You decide to collect some background information about her known historical antibodies before beginning the work-up knowing that she has a history of anti-E, anti-K, anti-Jk^a, anti-Fy^a, anti-M and anti-Le^a. When you complete the work-up, you note that the anti-Jk^a antibody is no longer detectable. Can the patient receive red blood cells that contain the Jk^a antigen? Why or why not?Many ELISAs that are used to identify the presence of antibody to viruses have a 3rd control (along with negative and positive). In between the rows of wells coated with antigen, are rows of cells coated with tissue culture used to grow the virus. In other words, patient serum is added to wells that contain antigen along with wells that contain tissue culture. Why do you think this might be necessary?
- You have 4 ml of an antigen solution, how would you prepare a 3-fold dilution series such that you will have at least 2 ml of each dilution? Please draw a picture and explain, I don't understand what it means by 3-fold.You have 4 ml of an antigen solution, how would you prepare a 3-fold dilution series such that you will have at least 2 ml of each dilution? Please draw a picture and explain, I don't understand what it means by 3-fold. Please explain, no copy paste asapWhat are the important considerations that you have to remember in antibody screening? What are the important considerations that you have to remember in antibody identification What is the role of antibody screening in pretransfusion compatibility testing?
- What are the ordered steps of an ELISA protocol? A. Add primary antibody->wash-> Bind sample to a surface ->Add substrate ->Add secondary antibody-enzyme conjugate ->wash B. Bind sample to support -> Add substrate -> Add primary antibody -> wash -> Add secondary antibody-enzyme conjugate -> wash C. Bind sample to a surface -> Add primary antibody -> wash -> Add secondary antibody-enzyme conjugate -> wash -> Add substrate D. Add secondary antibody-enzyme conjugate -> wash -> Add primary antibody -> wash -> Add substrate -> Bind sample to surfaceWhat antibody-based tests can you buy at your local pharmacy?You just received the properly labeled blood bank specimen on patient Aran Stark. You decide to collect some background information about her known historical antibodies before beginning the work-up knowing that she has a history of anti-E, anti-K, anti-Jk^a, anti-Fy^a, anti-M and anti-Le^a. Which antibody can be neutralized? Which antibody is destroyed with 0.2M DTT treatment? Which antibody reactivity is enhanced by acidification? Which of the antibodies that are typically IgG in nature are destroyed by enzymes? Which are enhanced by enzymes? Which of these antibodies have been known to cause hemolytic transfusion reaction? Which of these antibodies are known to react at room temperature? Which of these antibodies react best at 37C? * When you complete the work-up, you note that the anti-Jk^a antibody is no longer detectable. Can the patient receive red blood cells that contain the Jk^a antigen? Why or why not?