Q: What advantages do solid media offer for the isolation ofmicroorganisms?
A: Micro-organism are those that cannot be seen by the naked eye, but are visible under microscopes.…
Q: What is a mixed culture? A contaminated culture?
A: A microbiological culture, or microbial culture, is a technique for increasing microbial creatures…
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A: S.Aureus is the most common cause of abscesses, furuncles, and cellulitis in the skin and soft…
Q: How irradiations are helpful in food preservation? explain briefly
A: Food preservation Food preservation is a method of keeping food safe log longer period of time.…
Q: Why is mannitol salt agar (MSA) considered both a selective and differential agar for staphylococci?
A: We’ll answer the first question since the exact one wasn’t specified. Please submit a new question…
Q: Name two ways that pure, mixed, and contaminated cultures aresimilar and two ways that they differ…
A: Microorganisms are isolated and cultured for various reasons. Culture is the observable growth that…
Q: Are the large numbers of microorganisms found in the mouth cause for concern? Explain.
A: We are authorized to answer one question at a time.Since you have not mentioned which one,I am…
Q: Why is the culture medium referred to as a highly enriched laboratory soil?
A: Culture medium is referred to as highly enriched laboratory soli because it provides all the…
Q: Why is it important for a microbiology laboratory technician to use aseptic technique?
A: The majority of the microbes onto which scientists work in laboratories are known pathogens.
Q: What is the importance of agar plates and bacteria cultures in food microbiology?
A: There are different culturing techniques such as agar plates, broth culture, stab culture and solid…
Q: Discuss the importance of some bacteria to undergo starvation during stationary phase and importance…
A: The importance of some bacteria to undergo starvation during stationary phase is that : In…
Q: The following media are used to grow bacteria for various purposes. Match the examples of media that…
A: MacConkey Agar: - Selective Growth Medium Malt Extract Agar:- Complex Medium
Q: List the two main types of culture media and how each differs and the types of microbes which grow…
A: Culture media contains all the elements which the bacteria needs for growth and is not selective in…
Q: Name the 5 main sources of external contamination associated with spent culture media during…
A: Biopsy-based embryo testing for genetic abnormalities, aneuploidy, or preimplantation genetic…
Q: How can the enrichment culture technique be used medically?
A: Bacteria require appropriate environmental conditions for its growth. Solid or a liquid preparation…
Q: Why is the membrane filter method preferred to the most probable numbermethod (MPN) for the…
A: Most probable number (MPN) is a method used to estimate the numbers of bacterial cells in a water…
Q: discuss the significance of the different aseptic techniques utilized in maintaining sterility of…
A: The laboratory that carries out growth of prokaryotic cells or eukaryotic cells in physiological…
Q: How is the CTSA (Coconut tryptic soy agar) growth medium designed to meet the nutritional need of…
A: Coconut trypic soy agar media are general-purpose, non-selective media that provide enough nutrients…
Q: Why are complex media preferable to chemically defined media for routinecultivation of…
A: Microorganisms require nutrients as an energy source and certain other environmental conditions to…
Q: Why is CFU more applicable to a culture of Streptococcus than to a culture of E. coli?
A: Colony-forming unit (CFU) represents the number of colonies formed on the media plate from one…
Q: Is the Peptone Iron Agar (PIA) a complex or defined medium? Explain based on its composition. Is the…
A: Depending on their composition, media can be classified as defined and undefined media. Defined…
Q: Match the description to the correct medium This medium is enrichment for fastidious [Choose ]…
A: It is important to grow microorganisms outside the body for the study of their characteristics and…
Q: Name five functional traits of microorganisms. What dose the functional diversity describe? Do we…
A: Functional traits of the microbes describe individually expressed phenotypes or characteristics of…
Q: Why might viable cell cultures be of more use in microbialtaxonomy than preserved specimens?
A: Microbial taxonomy is a field of biological sciences that deals with the grouping of microorganisms…
Q: Why do you think the continuous culture has higher chances of contamination than batch and fed-batch…
A: Continuous culture may be a set of techniques wont to reproducibly cultivate microorganisms at…
Q: You were given a mixed nutrient agar broth culture of bacteria 1a. How will you determine the…
A: A mixed bacterial culture consists of more than one bacterial cell types growing together. On the…
Q: Using the Table above as a guide, indicating what media could be used to differentiate the…
A: Introduction : The bacterium Staphylococcus epidermidis is gram-positive. It is a natural component…
Q: If you prepare culture media plates 2 days ahead of the laboratory schedule, can you still use the…
A: Preparation of culture media and inoculation are two of the very sensitive steps in any…
Q: What are the primary purposes of pasteurization, and what isultrapasteurization?
A: The method of killing the pathogenic microorganisms such as viruses, bacteria, spores, and fungi is…
Q: What appropriate method should be used in controlling microbial growth in each of the following? 1.…
A: The control of microbes and other biological agents from organic substances is called sterilization.…
Q: Can simply observing the colonies formed on agar plates and the results of a few biochemical tests…
A: These are biochemical test is to perform to identify different type of bacteria.
Q: Using aseptic techniques, list the general procedure of inoculating a broth from a plate culture.
A: Aspetic techniques that you should follow during inoculating a broth from a plate culture are:…
Q: Identify culture methods and conditions used for blood, wound,urine, fecal, and genital specimens.…
A: To detect the pathogens present in the body, the specimen is collected by withdrawing blood, swabs…
Q: Define the following terms; streak plate method, and selective media. State the purpose and…
A: Plating is a microbiological method used to separate microorganisms found in a sample. It is used to…
Q: Which of the following medium would be most appropriate for the bacterial cultures from stomach? a.…
A: Microorganism culture out of their natural environment in pure form in the laboratory has been…
Q: What compounds or chemicals might be added to a medium to make it selective?
A: Culture medium or growth medium is a liquid, solid, or semisolid designed to support the growth of…
Q: How does the agar dilution method differ from streaking toobtain isolated colonies?
A: Both these are methods to culture microorganisms and have their respective pro and cons as mentioned…
Q: Describe conventional techniques used to isolate single colonies and establish a pure culture
A: Answer: Introduction: Pure culture a culture contains growth of only single organism without other…
Q: Do pure cultures have to be used in a chemostat?
A: Chemostat is a culture chamber where microorganisms are cultured under static environment. The…
Q: Give the use and advantages of the following cultures for the examination of colony characteristics:…
A: Culture media: a. Culture media provides an environment that supplies all essential nutrition for…
Q: selective agar media for gram-positive bacteria?
A: Phenyl Ethyl Alcohol Blood Agar is a selective media for gram positive bacteria.
Q: discuss why a mixed culture cannot be used to inoculate a differential media such as the tripple…
A: A mixed culture is one that contains more than one type of organism growing in a sterile medium. The…
Q: describe how the pure culture of mycobacterium ulcerans should be inoculated with the culture
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Q: Which culturing method provided the clearest results, the semisolid medium tube or the soft agar…
A: Agar is a jelly-like substance obtained from red algae. It is a mixture of two components namely…
Q: How is the CTSA (coconut tryptic soy agar plate) growth medium designed to meet the nutritional need…
A: TSA is a general-purpose plating medium that can be used to isolate, cultivate, and maintain a wide…
Q: What is meant by the word sterile? Why is aseptic techniquenecessary for successful maintenance of…
A: Microbes are all pervading. They are found everywhere, in air, water, any substratum, inside and on…
Q: What are the primary uses of the following preparations of culture media? Agar regular slant Agar…
A: Hello. Since your question has multiple sub-parts, we will solve the first three sub-parts for you.…
Q: Describe colonial characteristics of the organisms grown on the different culture media according to…
A: Microbiology is the study of microorganisms that are invisible to the naked eye. The microorganisms…
Name six basic nutritional requirements supplied in all culture media.
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- The thermal death point is best described as:(a) The highest temperature that will support growth of amicrobial culture(b) The length of time required for killing 100% of mi-crobes in a 24-hour culture that is heated to 100°C(c) The temperature at which a previously growing 24-hourculture will begin to die(d) The temperature at which 100% of the microbes in a24-hour culture will be killed in 10 minutesYou are transferring a bacterial culture from a tube of broth to a Petri dish containing trypticase soy agar. Please list the steps that are necessary to make the transfer successfully and with minimal risk. of contamination.Differentiate the various types of culture media for the growth ofmicroorganisms: synthetic, complex, defined, selective and differential.
- Kligler’s iron agar and SIM are multiple test media.. Both media are stabbed but for different reasons. Explain.Based on the knowledge acquired about the conservation and propagation of microorganisms, propose methodologies for:a) conservation of stock culture (used infrequently) of baker's yeast (S. cerevisiae);b) conservation of the working culture (used daily) of the baker's yeast;c) activation (1st stage of propagation) of the work culture;d) propagation of cultures until inoculating a 100 L reactor toi) production of baker's yeast;ii) ethanol production.discuss the significance of the different aseptic techniques utilized in maintaining sterility of the cell culture lab.
- Illustrate the step by step method of preparing culture media.Choose two from the following culture preservations that are best to use when only an ordinary refrigerator is available for storage: a) Drying b) Mineral oil overlay c) Freezing with liquid nitrogen d) Lyophilization e) Periodic transfer to fresh mediumCreate a graph of this spectrophotometric data (using Excel or the graph paper below), labeling the four stages of the growth curve- lag, log (exponential), stationary, death. Be sure to label your axes properly and give your graph a title Time Culture Sampled % Absorbance 0 min 5% 30min 7% 60min 9% 90min 15% 120min 25% 150min 37% 180min 49% 210min 50% 240min 49% 270min…