Multiple choice DNA polymerases can only elongate from: -Free 3’ hydroxyl groups -The area ahead of the sliding clamp -Okazaki Fragments -The 5’ end of the lagging strand and The ______ of the EGFR allows coupling with another monomer. -Dimerization arm -Conformation arm -Polarization arm -Signaling arm

Multiple choice DNA polymerases can only elongate from: -Free 3’ hydroxyl groups -The area ahead of the sliding clamp -Okazaki Fragments -The 5’ end of the lagging strand and The ______ of the EGFR allows coupling with another monomer. -Dimerization arm -Conformation arm -Polarization arm -Signaling arm

Human Heredity: Principles and Issues (MindTap Course List)

11th Edition

ISBN:9781305251052

Author:Michael Cummings

Publisher:Michael Cummings

Chapter8: The Structure, Replication, And Chromosomal Organization Of Dna

Section: Chapter Questions

Problem 23QP: How does DNA replication occur in a precise manner to ensure that identical genetic information is...

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Using the picture below, match each letter (A-E) to 5' or 3' DNA polymerase molecule Parental DNA Replication fork A [ [ Choose ] [ [ Choose ] [ [ Choose ] D [ [ Choose ] E [ [ Choose ] > > > > B.
Compare and contrast the properties of DNA polymerase and RNA polymerase. Drag the appropriate items to their respective bins. can proofread using a 3'-to-5' exonuclease activity polymerize in a 5'-to-3' direction Only RNA can initiate strand synthesis catalyze phosphodiester bond formation to polymerize nucleotides into nucleic acids Only DNA use deoxyribonucleotide triphosphates as substrates can only extend an existing strand Both Reset Help dependent on a DNA sequence template use ribonucleotide triphosphates as substrates
A replication fork is shown below. The primary enzyme that catalyzes replication is [ Select ] . That enzyme also [ Select ] The leading strand would be the [ Select ] . Helicase is a(n) [ Select ] In this picture, helicase would be moving in the [ Select ] direction. 3' > >
Match the proteins with their function in DNA replication. unwinds DNA at replication fork forms covalent phosphodiester bonds to join DNA fragments remove primer and replace the RNA with DNA, also has 3'-> 5'exonuclease activity for proofreading synthesizes DNA by adding to the 3' end of the growing strand of DNA adds RNA primer uses an RNA template to facilitate extension of the telomeres in eukaryotes helicase DNA ligase DNA polymerase II introduces a double-stranded break ahead of the replication fork and swivels the cleaved ends around the central axis to relieve the stress of helix unwinding DNA polymerase III DNA primase telomerase DNA Gyrase (topoisomerase)
Which of (a)-(d) is complementary to the DNA segment 5'-ATGAGCCAT-3'? * 5-ТАСТССGTА-3' 5-ТАСТСGGTА-3' 5'-TCATCGGTA-3' 5'-TACTGCGTA-3'
Match the proteins with their function in DNA replication. unwinds DNA at replication fork forms covalent phosphodiester bonds to join DNA fragments remove primer and replace the RNA with DNA, also has 3'-> 5'exonuclease activity for proofreading synthesizes DNA by adding to the 3' end of the growing strand of DNA adds RNA primer uses an RNA template to facilitate extension of the telomeres in eukaryotes introduces a double-stranded break ahead of the replication fork and swivels the cleaved ends around the central axis to relieve the stress of helix unwinding helicase [Choose ] ✓ helicase telomerase DNA polymerase II DNA polymerase I DNA polymerase III DNA primase DNA ligase D DNA Gyrase (topoiso- merase) exonuclease
In the following diagram of a replication fork, primers are shown as thick black lines and newly synthesized DNA is shown as squiggly lines. Enzymes are shown as circles or boxes. Click on DNA polymerase I 3' 5' 7 c 34 % G Search or type URL 3' MacBook Pro & 5' + (: * II in 3 5' 3' +
How does DNA replication occur in a precise manner to ensure that identical genetic information is put into the new chromatid? See Figures 8.12 and 8.13. FIGURE 8.12 In DNA replication, the two polynucleotide strands uncoil, and each is a template for synthesizing a new strand. A replicated DNA molecule contains one new strand and one old strand. This mechanism is called semiconservative replication. FIGURE 8.13 A close-up look at the process of DNA replication. (a) As the strands uncoil, bases are added to the newly synthesized strand by complementary base pairing with bases in the template strand. The new bases are linked together by DNA polymerase. (b) DNA synthesis can proceed only in the 5 3 direction; newly synthesized DNA on one template strand is made in short segments and linked together by the enzyme DNA ligase.
Shade A if it is True and B if it is False A. True B. False 24. Every phosphate is connected to a sugar 25. Uracil is present in RNA replication 26. 2 haploid cell is the end product of DNA replication 27. Complimentary base pairing is compose of 7 nucleotides 29. New strands are copied and formed from separated strands of DNA 30. 3' to 5' end, 5' to 3' end are the directionality of DNA strands
ANALYSIS ANALYSIS: A DNA strand undergoes all the process included in the central dogma. The DNA strand used as a template is given below: Parent strand DNA: DNA daughter strand: 5-AGA-ACT-AAA-CТА-ТСG-СTT-CGT-3' hnRNA: MRNA: original protein: mutated mRNA: mutated protein: second letter A G UUUPhe UCU) UCC UAU UGU Cys UUC U UUA UAC Tyr UGC Ser UAA stop UGA stop| A UAG stop UGG Trp UCA Leu UUG UCG G CUU CCU CAU CGU His CAC САА CUC CC CGC Leu Pro Arg A CUA ССА CG CGA Gln CAG SO AAU CUG CGG AUU ACU AGU AGC Ser A Asn AUC lle A AUA ACC AAC Thr AAA AAG Lys GAU АСА AUG Met ACG AGG Arg GUU GCU GGU U Asp GUC GGC GCC GCA GCG GAC Val GUA Ala Gly A GAG Glu GGG GAA GGA GUG G Translating the unmutated MRNA that was obtained after splicing, the original protein sequence will be? The original protein is [A] (For your answer, use the one-letter symbol (all caps) for the amino acid residues separated by dashes, e.g. C-A-S-H). first letter third letter
Please put these steps in the correct order.Is this for leading or lagging strand RNA nucleotides are replaced with DNA nucleotides (proofreading while they go) Okazaki fragments are started. Single stranded DNA binding proteins bind. Okazaki fragments are finished until reach end of replication fork RNA primer degraded Initiator proteins bind dsDNA Okazaki fragments joined together dsDNA pried apart.
The figure below depicts various elements of the eukaryotic replication machinery in action. Enter the name for the protein depicted by each box. Box A Box B Box C Box D Box E Box F DNA polymerase on lagging strand (just finishing an Okazaki fragment) F Maintains polymerase association with DNA Enzyme extends separation of DNA strands Synthesizes RNA fragments that hybridize to DNA Relaxes supercoiled DNA ahead of replication fork Maintains DNA is single stranded state Promotes binding of processivity factors to DNA Newly synthesized strand pocoar Leading-strand template A New Okazaki fragment RNA primer E Lagging-strand template DNA polymerase on leading strand B C D Saaragon - Next Okazaki fragment will start here Parental DNA helix