lỗ determine if the antibiotic resistance in MH1 was carried on a plasmid, you first isolate the plasmid in MH1 using the plasmid DNA purification technique. Then, you transform bacteria that are not resistant to penicillin/ampicillin with the plasmid isolated from MH1. For the bacterial transformation experiment, you set up the three controls listed below. Match each control with its appropriate purpose (i.e. what it is controlling for) Please note: Transformed bacteria are bacteria that received the plasmid from MH1 and untransformed bacteria are bacteria that did not receive a plasmid. Testing to ensure that the bacteria used in the transformation experiment are viable (i.e. can grow on LB media) [Choose) after transformation. [ Choose] Untransformed bacteria plated on LB only plate Transformed bacteria plated on LB only plate Testing to ensure that the bacteria used in the transformation experiment are viable (ie. can grow on LB media) before transformation. Untransformed bacteria plated on LB+Amp plate Testing to confirm that ampicillin can kill the bacteria used in the ( Choose] transformation experiment.
lỗ determine if the antibiotic resistance in MH1 was carried on a plasmid, you first isolate the plasmid in MH1 using the plasmid DNA purification technique. Then, you transform bacteria that are not resistant to penicillin/ampicillin with the plasmid isolated from MH1. For the bacterial transformation experiment, you set up the three controls listed below. Match each control with its appropriate purpose (i.e. what it is controlling for) Please note: Transformed bacteria are bacteria that received the plasmid from MH1 and untransformed bacteria are bacteria that did not receive a plasmid. Testing to ensure that the bacteria used in the transformation experiment are viable (i.e. can grow on LB media) [Choose) after transformation. [ Choose] Untransformed bacteria plated on LB only plate Transformed bacteria plated on LB only plate Testing to ensure that the bacteria used in the transformation experiment are viable (ie. can grow on LB media) before transformation. Untransformed bacteria plated on LB+Amp plate Testing to confirm that ampicillin can kill the bacteria used in the ( Choose] transformation experiment.
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
Question
Transcribed Image Text:To determine if the antibiotic resistance in MH1 was carried on a plasmid, you first
isolate the plasmid in MH1 using the plasmid DNA purification technique. Then, you
transform bacteria that are not resistant to penicillin/ampicillin with the plasmid
isolated from MH1. For the bacterial transformation experiment, you set up the three
controls listed below.
Match each control with its appropriate purpose (i.e. what it is controlling for)
Please note: Transformed bacteria are bacteria that received the plasmid from MH1
and untransformed bacteria are bacteria that did not receive a plasmid.
Testing to ensure that the bacteria
used in the transformation experiment
are viable (i.e. can grow on LB media) (Choose)
[ Choose )
after transformation.
Untransformed bacteria plated on LB only plate
Testing to ensure that the bacteria
used in the transformation experiment
are viable (ie. can grow on LB media)
before transformation.
Transformed bacteria plated on LB only plate
Untransformed bacteria plated on LB+Amp plate
Testing to confirm that ampicillin can
| Choose
kill the bacteria used in the
transformation experiment.
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