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- Make a concept map/flow chart for this technique (Cellulose Tape Perianal Swab)Explain the importance of steam during the acid-fast staining procedure. Discuss the advantages of differential staining procedures over the simple staining techniques. List and briefly describe the functions/purpose of each of the different stains used in acid fast staining.uses of 1D and 2D polyacrylamide gel electrophoresis techniques.
- Put the following steps for running a gel in order. first Remove comb and put gel int v [ Choose ] Load samples into gel Let gel solidify for 15-20 minutes Connect electrodes and turn on power to run gel second Pour heated, liquid agarose into gel cartridge Remove comb and put gel into bottom of electrophoresis box Pour buffer into electrophoresis box to cover gel third fourth Pour heated, liquid agarose in v fifth Load samples into gel sixth Connect electrodes and turn vGel used in electrophoresis is/are: a. Agarose gel b. Polyacrylamide plain gel c. Polyacrylamide SDS impregnated Polyacrylamide gel (Sodium dodecyl sulphate)In outline form discuss the steps of Ziehl-Neelsen staining Complete the table for National Reporting Scale (Description) National Reporting Scale Result Description 0 +n 1+ 2+ 3+
- What are the differences among native gel electrophoresis, SDS-PAGE, isoelectric focusing, isotachophoresis, 2-D gel electrophoresis, and pulsed-field gel electrophoresis in terms of denaturants used, pH and electrolyte content, and application of electric field gradient?3) Define gel electrophoresis, including its theory and application. Describe the steps of running gel electrophoresis using the following image. More detailed reading: https://www.sciencedirect.com/topics/medicine-and-dentistry/agar-gel-electrophoresis POWER SUPPLY CATHODE ELECTROPHORETIC BUFFER ANODE WELL SAMPLE AGAROSE GEL POWER SUPPLY CATHODE ANOCE HIGH MOLECULAR WEIGHT SPECIES LOW MOLECULAR WEIGHT ANALYTESSummarize the steps for general Hematoxylin and Eosin staining procedure through a flow chart.