Lactobacillus bulgaricus is the main bacterium used in the production of yogurt and requires an acidic environment to grow effectively. To prepare an agar solution of this bacterium, a 100.0 mL 0.100 M buffer solution pH 5.00 must be prepared. The only available reagents in the laboratory are solid benzoic acid (HC6H5COO, MM = 122.12), solid sodium bicarbonate (NaHCO3, MM = 84.01), 1.00 M HCl, and 1.00 M NaOH. Acid dissociation constants: Benzoic acid, HC6H5COO: pKa,1 = 4.19 Carbonic acid, H2CO3: pKa,1 = 6.37, pKa,2 = 10.32 Identify the most appropriate conjugate acid-base pair for the target buffer. Determine the amounts of the acidic and basic species in the buffer (in millimoles).

Chemistry: The Molecular Science
5th Edition
ISBN:9781285199047
Author:John W. Moore, Conrad L. Stanitski
Publisher:John W. Moore, Conrad L. Stanitski
Chapter15: Additional Aqueous Equilibria
Section: Chapter Questions
Problem 32QRT: The titration curves for two acids with the same base are shown on this graph. (a) Which is the...
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Lactobacillus bulgaricus is the main bacterium used in the production of yogurt and requires an acidic environment to grow effectively. To prepare an agar solution of this bacterium, a 100.0 mL 0.100 M buffer solution pH 5.00 must be prepared. The only available reagents in the laboratory are solid benzoic acid (HC6H5COO, MM = 122.12), solid sodium bicarbonate (NaHCO3, MM = 84.01), 1.00 M HCl, and 1.00 M NaOH.

Acid dissociation constants:

  • Benzoic acid, HC6H5COO: pKa,1 = 4.19
  • Carbonic acid, H2CO3: pKa,1 = 6.37, pKa,2 = 10.32
  1. Identify the most appropriate conjugate acid-base pair for the target buffer.
  2. Determine the amounts of the acidic and basic species in the buffer (in millimoles).
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