Increasing the saturation of the ammonium sulfate is a prerequisite in isolating a target protein that is rich in Cys and Tyr residues. Which of the following techniques should be considered in accurately quantifying the isolated protein? I. Running the isolated protein in a dialysis or GFC set up. II. Using Biuret or BCA assay as the colorimetric quantitation method. III. Using Bradford or Lowry assay as the colorimetric quantitation method. A. I only B. II only C. I and III D. I, II and III
Enzyme kinetics
In biochemistry, enzymes are proteins that act as biological catalysts. Catalysis is the addition of a catalyst to a chemical reaction to speed up the pace of the reaction. Catalysis can be categorized as either homogeneous or heterogeneous, depending on whether the catalysts are distributed in the same phase as that of the reactants. Enzymes are an essential part of the cell because, without them, many organic processes would slow down and thus will affect the processes that are important for cell survival and sustenance.
Regulation of Enzymes
A substance that acts as a catalyst to regulate the reaction rate in the living organism's metabolic pathways without itself getting altered is an enzyme. Most of the biological reactions and metabolic pathways in the living systems are carried out by enzymes. They are specific for their works and work in particular conditions. It maintains the best possible rate of reaction in the most stable state. The enzymes have distinct properties as they can proceed with the reaction in any direction, their particular binding sites, pH specificity, temperature specificity required in very few amounts.
pls explain
Increasing the saturation of the ammonium sulfate is a prerequisite in isolating a
target protein that is rich in Cys and Tyr residues. Which of the following
techniques should be considered in accurately quantifying the isolated protein?
I. Running the isolated protein in a dialysis or GFC set up.
II. Using Biuret or BCA assay as the colorimetric quantitation method.
III. Using Bradford or Lowry assay as the colorimetric quantitation method.
A. I only
B. II only
C. I and III
D. I, II and III
. Bradford Assay is most suitable to use when the extraction buffer is below the
target protein’s pI. This is so because the protein would be more
a. Positively charged allowing the CBB G-250 dye to bind via its sulfonate
groups.
b. Negatively charged allowing the CBB G-250 dye to bind via its sulfonate
groups.
c. Neutrally charged allowing the CBB G-250 dye to bind via its sulfonate
groups.
d. Zwitterionic allowing the CBB G-250 dye to bind via its sulfonate groups.
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