in the column chromatography of spinach extract Could you potentially see the usefulness of this method in a field outside of the organic chemistry lab? Explain why or why not.
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in the column chromatography of spinach extract Could you potentially see the usefulness of this method in a field outside of the
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- 1. Give the importance of carbohydrate fermentation test in biochemical testing and enumerate the enzymes that are involved. (5 points) 2. What are the substances added in the culture media utilized by the organism producing hydrogen sulfide? Give 2 other media used for the production of hydrogen sulfide. (5 points) 3. Explain the mechanism taking place in the hydrolysis of urea which leads to the formation of bluish red color. ( 5 points) 4. Discuss why human blood plasma will not always yield reliable results. (5 points)colored. Interpretation: () Lactose Observed that the addition of the Benedict reagent to the specimens, the test tube is placed in a 20-minute water bath, in which the sample's color precipitates as a silver- colored. Interpretation: (+) Sucrose With the addition of the benedict reagent to the samples, the test tube is placed in a 20- minute water bath. The color of the sample becomes translucent. Interpretation: (O Starch With the addition of the benedict reagent to the samples, the test tube is placed in a 20- minute water bath. The color of the sample becomes translucent. Interpretation: () This material should not be duplicated nor distributed without permission. BIOCHEM LABORATORY: CARBOHYDRATES GUIDE QUESTIONS: 1. Some of the test(s) you tried from this virtual lab are important basis for determining glucose levels of diabetic patients from blood and/or urine. Can you identify which are these tests and how is modern method used glucose monitoring different? (Show the reactions)These are SIM media. What does SIM stand for? Describe the reactions in Tubes A, B, C, D, E.
- Media - Possible Results Туре of Medium Original Color Any Chemicals Added to Visualize Reaction? Negative Result Positive Result Phenol Red Glucose Phenol Red Lactose Sulfur: Sulfur: SIM Indole: Indole: Motility: Motility: MR VP Citrate Slant Nitrate Broth 6.5% NaCi TSB Bile Esculin For the Fluid Thioglycollate Medium, draw the five possible outcomes in the sections below and label accordingly. (obligate aerobe, obligate anaerobe, facultative anaerobe, aerotolerant anaerobe, microaerophile) Fluid Thioglycollate Medium Novobiocin Sensitivity Test : Use the Difco Manual for the following and describe the possible outcomes: EMB: Mannitol Salt Agar:Table H. Reduced Sulfur Reaction Samples 1. Cysteine Observation 2. Casein 3. Albumin 4. Gelatin 5. hair 6. distilled waterCONTENTS OF REACTION CUVETTES: (Calculations in PreLab) [ONPG] Diluted ONPG 0mM None 0.25mL 0.5mL 0.75mL 1.0mL bloo 1.25mL 1.5mL Buffer Lactase 0.25mL 0.25mL 0.25mL 0.25mL 0.25mL 0.25mL 0.25mL 0.25mL 0.25mL 0.25mL 0.25mL 0.25mL 0.25mL 0.25mL *** HINT: To calculate [ONPG], cuvette #5 contains 1.0mL ONPG in a total of 2.0mL. (This is the same as a 1:2 dilution.) Therefore we divide the concentration of ONPG by 2 to get 5mM. 5mM Water 1.5mL 1.25mL 1.0mL 0.75mL 0.5mL 0.25mL None *** 4. To perform Lactase Assay with first cuvette ("OmM"): Clean cuvette with Kimwipe. Place cuvette into Spectro Vis with stripes in front in back. GET GROUP READY FOR TIMED ASSAY. AT THE SAME TIME: * Press Record (Green Triangle button). * Add 0.25mL of Lactase to cuvette. Use pipette to quickly mix liquid. WAIT 60 SECONDS! After the assay is complete: * In Experiment menu, select "Store Latest Run" THIS STEP IS NECESSARY! * Record data on the data table below. 5. Repeat Step 7 for all of your cuvettes. Record…
- Example of a Protein Purification Scheme: Purification of the Enzyme Xanthine Dehydrogenase from a Fungus Volume Total Total Specific Percent Fraction (mL) Protein (mg) Activity Activity Recovery 1. Crude extract 2. Salt precipitate 3. Ion-exchange chromatography |4. Molecular-sieve chromatography 5. Immunoaffinity chromatography 3,800 22,800 2,460 0.108 100 165 2,800 1,190 0.425 48 65 100 720 7.2 29 40 14.5 23 1.8 275 152.108 11 Calculate the specific activity of step#4. Note that percent recovery=% Yield.PART III - Table 3: Absorption Spectrum Data for Spinach Chloroplast Extract Absorbance Wavelength 620 nm 0.147 640 nm 0.058 660 nm 680 nm 700 nm 720 nm Wavelength 380 nm 400 nm 420 nm 440 nm 460 nm 480 nm Absorbance 0.759 0.923 Questions: 1.095 1.075 0.833 10.520 Wavelength 500 nm 520 nm 540 nm 560 nm 580 nm 600 nm 0.054 0.066 0.094 123 Graph: Plot the absorbance data on the graph below. Remember TAILS. Absorbance · 163 ·19 4 •514 · 166 1012 ·003 1. Your graph should have two "peaks." At the highest of the two, what wavelength was most strongly absorbed by the chloroplast pigments? What wavelength corresponds the top of the second peak? What wavelength was least absorbed by the chloroplast pigments? 2. Based on your observations, which wavelength (color) of light might be expected to generate the lowest rate of photosynthesis? 91A biochemist carefully measures the molarity of salt in 56. mL of photobacterium cell growth medium to be 82. mM. Unfortunately, a careless graduate student forgets to cover the container of growth medium and a substantial amount of the solvent evaporates. The volume of the cell growth medium falls to 3.1 mL. Calculate the new molarity of salt in the photobacterium cell growth medium. Be sure your answer has the correct number of significant digits. ||mM Ox10
- 50 mg/50 ml kinetin stock is available You need 2.5 mg/l kn for Ms media calculate amount of kn is required? Explain the role of surface sterilising chemicals in aseptic culture initiation and function of sucrose in MS medium?Explain how to prepare 250 ml liquid and 500 ml solid form separately from LB (Luria Bertani) medium whose components and concentrations are given below. Yeast extract 5 g/L Peptone 10 g/L NaCl 10 g/L Agar 1.5 g/LWhat is sterilization? Give specific examples of media which are sterilized by the following methods:A. Boiling or heating onlyB. Autoclave using steam under pressure.C. Inspissator
