In class, I mentioned that fructose is metabolized differently in the liver compared to glucose. Refer to the figure shown below to calculate the number of ATPS you would expect from the metabolism of fructose in the liver. Show your work! Fructokinase Fructose Fructose-1-P ATP ADP Aldolase B Dihydroxy- Glyceraldehyde ATP acetone phosphate Triose kinase Triose ADP phosphate - Glyceraldehyde-3-P isomerase Glycolysis
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- You have a crude lysate sample (CL) containing a mixture of six proteins (1, 2, 3, 4, 5, ẞ- galactosidase), and your goal is to obtain purified ẞ-gal. Some characteristics of these proteins are shown in the table below. Protein Alcohol dehydrogenase Carbonic anhydrase Insulin B chain Phosphorylase B Glutamic dehydrogenase B-galactosidase 45% Concentration of ammonium sulfate (AS) required for precipitation Molecular Weight (kDa) Isoelectric point (pl) 38 3.7 80% 65% 20% 30% 45% 28 4.8 4 5.3 98 6.8 49 9.5 115 5.3 You begin your purification by performing an ammonium sulfate (AS) precipitation. You add the appropriate concentration of AS to your CL sample, incubate overnight at 4°C, then centrifuge to generate a supernatant (AS-S) and pellet (AS-P). What concentration of AS will you use to precipitate Glutamic dehydrogenase? © 20% O 30% 45% 65% 80%There are eighteen (18) rows of boxes, for around 36-38 boxes total. Please see images attached. This is a pathway trace, with the final box showing "ribose 5 phosphate" as the end product. Any help is appreciated-thank you! glutamate to ribose 5 phosphate No a ketoglutarate dehydrogenase No pyruvate carboxylase No transketolase No transaminase You do get to have if needed: erythrose 4 phosphate asparate dihydroxyacetone phosphate bicarbonate ATP ornithine CO2Which of the following statements are True? Multiple answers: Multiple answers are accepted for this question Select one or more answers and submit. For keyboard navigation. SHOW MORE V If [1-14C) Ribose-S-phosphate is incubated with a mixture of purified transketolase- transaldolase-phosphopentose isomerase-phosphopentose epimerase and glyceraldehyde-3-phosphate Fructose-6-phosphate will be labeled in the 1 and 3 positions. a Starting with Glucose 6-phosphate labelled in carbon number 1 with radioactive carbon and taken through the axidative phase of the Pentose Phosphate Pathway and the first transaldolase reaction gives rise to a 7-carbon intermediate labeled with radioactive carbon in carbon number 1. Avidin is a protein that binds extremely tight to biotin. So avidin would be a potent inhibitor of Acetyl CoA Carboxylase. The two electrons derived from the a-Ketoglutarate Dehydrogenase complex come from dihydrolipoic acid. In Fatty Acid synthesis carbon atoms 15 and 16 of Palmitic…
- Choose the enzyme and cofactors involved in the reaction of fructose-1,6-bisphosphate to fructose-6-phosphate in gluconeogenesis. H H-TOPO Fo HOTH HDH HOH MOTOPOP K E H-TOH H OPO Problem 3 of 17 A B Q D E fructose-1,6-bisphosphatase phosphoglucose isomerase glucose-6-phosphatase phosphoglycerate kinase hexokinaseWhat is(are) the only active site(s) not used in the second round of fatty acid synthase? Select all that apply. Group of answer choices Acetyl-CoA ACP Transacylase Beta-Ketoacyl- ACP Synthase Beta-Ketoacyl- ACP Dehydrase Palmitoyl thioesterase Malonyl-CoA ACP Transacylase Enoyl-ACP ReductaseAMP is an activator of fructose 1,6-bisphosphatase (FBPase-1) True False
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- A sample of glycogen from a patient with liver disease is incubated with Pi, normal glycogen phosphorylase, and normal debranching enzyme. The ratio of G1P to glucose formed in the reaction mixture is 100. What is the patient’s most probable enzymatic defi ciency?If glucokinase has a higher Km for glucose than does hexokinase, but can only bind to D-glucose, while hexokinase can bind to several hexose sugars (like D-glucose, D-mannose, and D-fructose), then: glucokinase has both a higher affinity and a higher specificity for D-glucose than does hexokinase glucokinase has both a lower affinity and a lower specificity for D-glucose than does hexokinase glucokinase has a higher affinity but a lower specificity for D-glucose than does hexokinase glucokinase has a lower affinity but a higher specificity for D-glucose than does hexokinase all of the aboveWhat is the advantage for some tumors to stop expressing the genes for glucose-6-phosphatase, phosphoenolpyruvate carboxykinase, and fructose bisphosphatase?