if air can degrade a column and cause TC detector filaments to burn out, why do we use an air peak?

Q: What part fo chromatography is being descibe by the following? a phase that is fixed in place either…

A:

Q: Why was it important that you marked the origin line and component locations on the paper…

A: Pencil is made up of either graphite or lead and it does not have any interaction with the mobile…

Q: How can controlled potential coulometry detect the analyte?

A: Controlled potential coulometry, as the name suggests, is a coulometry method that measures the…

Q: Explain how the Rf (Retention Factor)-values can be in agreement or disagreement with the “like…

A: Interpretation -  To explain all about that how the Rf ( Retention factor ) - values can be…

Q: two general methods for improving the resolition of two  substances on chromatography column

A:

Q: What is the type of column used in chromatography (HPLC) and it's applications and mechanism

A: It is as follows

Q: Which of the following matches is/are incorrect regarding the paper chromatography experiment? a.…

A:

Q: Explain about Liquid Chromatography technique.

A:

Q: How many theoretical plates produce a chromatography peak eluting at 12.83 min with a width at…

A: The no. of theoretical plates can be calculated as: Where N is the no. of theoretical plates, tr is…

Q: in what situation does one use  thin and/or column chromatography? You can any of theme for anything…

A: Chromatography is an analytical technique to separate mixtures into it's components.  It is also…

Q: Why do we need to add sand on top of the silica in column chromatography? What could happen if we…

A: The presence of different compounds in the mixture is separated by employing the separation methods.…

Q: What is the impact of the shape of the solvent front in CE (Capillary Electrophoresis)  compare to…

A: Capillary electrophoresis (CE) is an analytical technique that separates ions based on their…

Q: (5). Write short notes on molecular exclusion chromatography with representative picture.

A: Molecular exclusion chromatography, otherwise called Size-exclusion chromatography (SEC), is a…

Q: Explain how the charged aerosol detector used

A: Charged aerosol detector: The detector, which is used to detect (measure) the amount of charged…

Q: Why is splitless injection used with purge and trap sample preparation?

A: The collection of all of the analyte from the unknown and to inject whole of the analyte into the…

Q: Give Four main precautions need to be considered when carrying out the following.

A: ANSWER IS :

Q: Explain how solid-phase microextraction works. Why is cold trapping necessary during injection with…

A:

Q: How does temperature, flow rate of carrier gas, boiling point and column suupport affect gas…

A: Explanation. Retention time-     Retention time is the amount of time a compound spends on…

Q: The eluent is to be below the 1.5 cm line on the chromatographic paper. Describe the expected…

A: Introduction- Thin layer chromatography(TLC) is a separation technique where the compounds are…

Q: Which type of gas chromatographic column consists of a tube that is densely packed with uniform,…

A: An analytical technique which is used to separate components present in a mixture and to determine…

Q: How resolution in HPLC analysis can be improved?

A: HPLC i.e  high-performance liquid chromatography is an analytical method which is being used to…

Q: calculate the following for each sample gas chromatograph. a) retention time for each peak (label…

A: Gas chromatography is a technique for separating and analyzing compounds that can be vaporized…

Q: Compare between open tabular column and packed columns in gas chromatography?

A: Gas chromatography is a technique which involves the sample being vaporized and then injected into…

Q: In thin-layer chromatography, what will be the result if the solvent level is higher than the sample…

A: A multiple choice question based on thin layer chromatography, which is to be accomplished.

Q: SUGGEST REASONABLE CLASSIC COLUMN DIMENSIONS FOR CHROMATOGRAPHING 0.5G OF SAMPLE ON ALUMNIA

A: The size of the column decide how much accurate is the separated product. The larger and wider size…

Q: a pigment in the thin layer chromatography traveled beyond or farther than the solvent front.…

A: As a pigment in the thin layer chromatography traveled beyond or farther the solvent front therefore…

Q: Why do we use a makeup gas for some gas chromatography detectors?

A: The method of separation of components present in the mixture is termed as chromatography.…

Q: Why should the syringe be removed from the sensor when setting the initial volume for each trial?

A: Option C

Q: A mixture of A and B is injected into a liquid chromatographic column with length of 45 cm. The…

A: given- length of the plate= L = 45 cm plate height = H = ?

Q: What is the application of chromatography (HPLC) and it's mechanism ?

A: Answer.

Q: What error is introduced into the determination of an Rf value if the top is left off the developing…

A: In chromatographic technique, the components of a mixture are separated by using a developing…

Q: Solve this

A: Solid phase extraction is technique of preparing sample with help of solid adsorbent. It is used to…

Q: In HPLC, why is purging or initialization being done before analysis of samples, explain.

A: ✓basically following two reasons for using purging and initialization during HPLC sample preparation…

Q: A HPLC column had a sample (0.10ml) loaded onto it. The detector volume showed 0.15 mL. From this…

A: Before starting any troubleshooting, whether it is related to instruments or columns, it isessential…

Q: Match column A with column B. v Mobile phase: NON-POLAR Stationary phase: NON-POLAR v mobile phase -…

A:

Q: the efficiency of a chromatographic column improves the measure that a.increases the height of the…

A: To determine what improves the efficiency of a chromatographic column:

Q: At the completion of the experiment, a student measures the solvent front to be 55 mm from the…

A:

Q: select

A: We know that A detector is a device that converts an amount of radiation into some other measurable…

Q: If the selectivity factor is equal to 1, the peaks are separated. True or False?

A: The given statement is If the selectivity factor is equal to 1, the peaks are separated.

Q: How can you ensure “good quality” peak separation in high performance liquid chromatography (HPLC)?

A: In HPLC , different kind of columns are used to separate to isomers. So, here by using trial method…

Q: Name two general methods for improving the resolution of two substances chromatographic column.

A: Good resolution columns provide a great separation of the components by a column chromatography.…

Q: In thin layer chromatography, changing the mobile phase will alter the Rf value of a compound. O…

A: This can be solved as follows

Q: Explain why it is needed to filter the solvent before introducing it to the HPLC system

A: Basically there are following two reasons for using filtration during HPLC sample preparation are…

Q: How to create a better chromatographic separation(supercritical fluid) without changing the column…

A: Please find your solution below : Chromatography is an analytical technique which is used for the…

Q: Compare thin layer chromatography and paper chromatography technique with aspects such as…

A: Chromatography is the laboratory technique for the separation and isolation of a variety of samples…

Q: Does the amount of radiation (cpm) increase or decrease as the distance between the sorce and the…

A: Amount of radiation between the source and sensor is determine using the inverse square formula:…

Q: In column chromatography, a) what constitutes the stationary phase, and b) what is the mobile phase…

A: By definition, chromatography is a separation technique in which a sample is equilibrated between a…

Q: Which of these is NOT an electrochemical measurement technique? O chronopotentiometry O column…

A:

Q: Should you increase the diameter or length of the chromatography colum for increasing throughput?

A:

Q: Comments on choice of a paper and solvent are main back bones of paper chromatography.

A: Paper Chromatography is mailny based on the choice of paper and solvent used. In this technique,…