How do I find the amount of substrate consumed using the given concentration of substrate, measured absorbance, and standard curve? The video I watched says compare the measured absorbance to the standard curve. How do I do that?
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How do I find the amount of substrate consumed using the given concentration of substrate, measured absorbance, and standard curve? The video I watched says compare the measured absorbance to the standard curve. How do I do that?
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- What is an absorption spectrum? The following graph is the visible absorbance spectrum of an indicatorsolution. How can we conclude about the color of this sample?a)Determine the amount of X (in gram) in order to prepare 100 mL of 50 mM stock solution of X. Show your work. (MW of X= 225) b)Determine the volume required (mL) from the stock solution X in (i) to prepare 500 mL of MS medium containing 5 mg/L X. Show your work.Absorbance is directly proportional to glucose concentration for both routine clinical glucose spectrophotometry methods: glucose oxidase method and the hexokinase method. For each one, name the final product measured in each test reaction, which is proportional to the sample glucose concentration. A.) glucose oxidase: B.) hexokinase:
- During cell couting, when the data are plotted in an ordinary graph or on a cross-section paper, why is the log value of the cell concentration used?If 3.8% (w/v) of sucrose is needed in the media, how much of sucrose is required to prepare the stated volume of media: a) 1000 mL b) 250 mLThe standard curve to determine the amount of betacyanin is shown below. You extracted a red pigment from a beet disc (the mass of a disc is 2 g) using 10 ml of 20% ethanol solution. You measured absorbance of the solution above the beet disc every minute for a total time of 20 minutes. The increase in absorbance was linear during a period of time from 1 min to 10 min. The absorbance at 10 min was 0.8. Calculate the amount of betacyanin extracted from 1g of a beet tissue per minute. Explain your calculations. You can use Excel or a calculator.
- Why are quantitative absorbance measurements made at lambda max?Using a P-1000 micropipette, you transfer 800 microliter of ddH2O to a beaker and measure its weight. It turns out to be only 780 milligram; what is the percent error of this micropipette?The Michaelis-Menten constant can be determined using the Lineweaver-Burk plot and is equal to twice the Vmax. can be determined using the Lineweaver-Burk plot and is equal to the substrate concentration at Vmax/2. can be determined using the Lineweaver-Burk plot. O is equal to the substrate concentration at Vmax/2. O is equal to twice the Vmax
- What is meant by the term plating efficiency?Calculate the volume of BSA stock that will be required to make the standard solutions needed to create the BSA standard curve. Be sure to show your work and include the volume of 0.02 M phosphate buffer required to reach a final volume of 1 mL. From a 2,000 μg/mL BSA stock, create 1 mL of each of the following stock solutions in 0.02 M phosphate buffer using individual microcentrifuge tubes: 50 μg/mL, 250 μg/mL, 500 μg/mL, 1,000 μg/mL, 1,250 μg/mL, 1,500 μg/mL. Be sure to properly label all the microcentrifuge tubes before creating the standards.What is the relationship of concentration and absorbance based on the table and what are the changes in the absorbance value mean?