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How column is developed and solvent selection is done in HPLC?
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- What are the properties that determine retention time in the Chromatography process? Please shortly answer at your own words. Answer should be to the point ( specific 5-6 lines).What is quantitative transfer and why is it used?Column Chromatography Alumina Chromatography Mixture 9:1 Hexanes:Ether 8:2 Hexanes:Ether 1:1 Hexanes:Acetone Amount Used 3.962 g 0.143 g 9.50 mL 9.50 mL 11.00 mL Additional Observations (Color, etc.) BIU X₂ X² → BI IU X₂ X² → BI IU X₂ X² → BI IU X₂ X² → BIU X₂ X² →
- In HPLC, fill in the blanks with the choices below: - chromatogramretention timevoid volume markernumber of theoretical platespeak widthplate height Resolutioncapacity factorreduced retention volumeselectivity - 1. This chromatographic parameter is a measure of efficiency ____.2. A high value of the _______ means high efficiency.3. ______ is both a measure of selectivity and efficiency.4. To measure the void time a _______ is use.5. A higher value of _______ means higher HETP.6. Another term for retention factor is _______7. _______ is ability of the chromatographic system to discriminate differentanalytes.8. The plot of the detector response vs. time is called a _______9. _______ distance of the peak maxima from the injection point expressed intime units.10. The difference between the retention volume and the void volume is called _____Brief discussion of column chromatographic separation (solvent polarity, elution ofcompounds etc.)What are the mandatory components required for column Chromatography? Please shortly answer at your own words. Answer should be to the point ( specific 4-5 lines).