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how can you determine aluminum, lead and zinc in a specimen using a microchemical technique?
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- A student performs a separation by TLC on a silica get plate that results in three spots. Ared spot is measured 23 mm away from the original sample spot, a blue spot is 42 mmaway, and a yellow spot is 49 mm away. The distance from the original spot to where thesolvent stopped is 55 mm.a) Calculate the Rf value for each component in the mixture. (Show your work and reportthe correct number of significant figures.) b) The silica gel plate is polar. Rank the three components in the mixture from lowest tohighest polarity. Explain your answer.a) What is the dilution factor if you add a 1ml aliquot of a specimen to 99ml of diluent? b)How to make 500ml of a 1:250 dilution?The following image is a scheme for serial dilutions prepared for spectrophotometric analysis. If the stock solution concentration is 0.05 % (v/v) can you calculate the other tube’s concentrations in % v/v? I've used this with direct dilutions, how would I use this on serial dilutions?
- Provide the Experimental procedure of the following: Example: Ninhydrin Test -The samples needed in this test were 1% casein solution, albumin and gelatin and the reagent used was ninhydrin (Triketohydrindenehydrate or Triketohydrin hydrate). Test tube, test tube stand, pippete, water bath, and spectrophotometer were the materials and equipment used. Ninhydrin reagent in 1-2 drops was added to 0.5 ml of the sample with care. It was mixed and heated to boiling for 1-2 minutes. Then, it was allowed to cool to record the observation. Test that needs Experimental procedure: 1. Heat denaturation Samples: Albumin, gelatin 2. Isoelectric Precipitation Samples: Casein 3. Concentrated Mineral Acids Samples: Albumin 4. Organic Solvents Samples: AlbuminCan paper chromatography be used to separate and identify very volatile substances? Explain your answer.What special precautions must be taken when using the oil immersion (100x) objective?
- What are the mechanisms of samples separation work in Thin layer Chromatography? Please shortly write at your own words. Answer should be to the point (5-6 lines maximum).In a spectrophotometer, what should be the appearance on the graph if the sample is pure? presence of salt and protein?The nurse notes the patient's IV pump is set at 12 mL/hr. The IV bag holds 20 units of Pitocin mixed in 500. mL of Lactated Ringers. How many mu/min is the patient receiving. Note that mu is milliunits. 6mu/min 48 mu/min 12 mu/min 0.48 mu/min 8 mu/min
