For the reaction E + S <> ES, what is the [E] if [S] = 0.18 mM, [ES] = 133 uM, and the Kd = 15 uM? 11,000 UM 20 uM 11 uM 180 uM
Q: Which of the following is the most efficient enzyme An enzyme with a high kcat and a high Km. An…
A: Enzymes play a fundamental role in biological processes by catalyzing chemical reactions, thereby…
Q: The approximate thickness of the phospholipid bilayer is 10A 1 micro M 50A 150A 1nM
A: There are four classes of biological macromolecules - nucleic acid, proteins, lipids and…
Q: Rank the following fatty acids from lowest melting temperature to highest melting temperature. (A)…
A: Fatty acids are carboxylic acids with a hydrocarbon chain ranging from 4 carbons to 36 carbons. The…
Q: In the absence of an enzyme, a reaction has a Keq = 10¹5. i. Is AGO for the reaction + or -? ii.…
A: In a general reaction such as:aA + bB ⇌ cC + dDAt equilibrium (steady state), the concentration of…
Q: The reaction coordinate diagram shown below is for the mitochondrial enzyme ATP synthase. G KJ/mol…
A: A reaction coordination graph is a graphical representation of the free energy changes that occur…
Q: is the sum of all chemical reactions in the body. It allows the body to convert energy from oods…
A: Metabolism is a fundamental and intricate biochemical process that underpins the functioning of…
Q: Given the following atomic weights, what is the molecular weight of the following triglyceride if n…
A: Fatty acids are carboxylic acids with a hydrocarbon chain ranging from 4 carbons to 36 carbons.…
Q: People are very interested in understanding how prions fold. (Prions are proteins that begin…
A: 1. To determine which protein, mouse or bovine, will be more "folded" at 310K, we need to compare…
Q: Assume a protein is composed of 120 amino acid residues and that each amino acid can have three…
A: Proteins are primarily polypeptide chains. A polypeptide chain is formed by amino acids. Amino acids…
Q: Briefly list out the chemicals contributing the anti-oxidative activities found in green tea.
A: Green tea is the most widely consumed beverage, following water.The chemicals present in green tea…
Q: 2. You made a pH=9.8 buffer solution by mixing NaOH and glycine to give a solution that is 0.20 M in…
A: In order to solve (d), first we need to calculate (c). So, here is a brief and comprehensive…
Q: Which of the following would be a valid symbol identifier of the fatty acid shown below? HO |…
A: There are four classes of biological macromolecules; proteins, nucleic acids, lipids and…
Q: HO-CH2 НО ОН H 0 Н но-сн, Н ОН H ОН Н Н ОН но-CH2 Н Н Н OH Н H OH HO-CH, H ОН H о он ОН H List the…
A: Monosaccharides are connected by glycosidic bonds. Glycosidic bonds are covalent bonds, formed when…
Q: Cold-blooded animals modulate the fatty acid composition of their membranes as a function of…
A: Answer :- Animals classified as cold-blooded, or ectotherms, depend on the outside temperature to…
Q: What is the relationship between lactate production and lactate remov
A: Lactate production and lactate removal are closely related processes in the human body, both of…
Q: 1a. Name and draw diagrammatically the series of mitochondrial electron transfer catalysts, starting…
A: ETC consists of four protein complexes called Complex I, II, III and IV that transport electrons…
Q: Draw a mechanism using the general features of a serine protease to explain how inhibitor x might…
A: Inhibitors can be broadly classified into reversible and irreversible inhibitors. If the bond…
Q: 10.) ANSWER THE FOLLOWING PROBLEM AND EXPLAIN YOUR ANSWER FOR BETTER UNDERSTANDING. Fill in the…
A: Amino acids are the building blocks of protein. Some amino acids in the protein undergo…
Q: Case Study: Enzyme Kinetics Data for new statin drug (inhibits HMG CoA reductase): I Substrate (UM)…
A: For a one-substrate enzyme-catalyzed reaction, the Michaelis-Menton equation shows the quantitative…
Q: 1a. If you wanted you could take a glucose molecule and convert it to pyruvate via glycolysis and…
A: Hello! Due to time constraints, we are able to provide the answer for the first sub part only. If…
Q: G protein-coupled receptors (GPCRs) and receptor tyrosine kinases (RTKs) are two basic receptor…
A: GPCR stands for G Protein-Coupled Receptor a type of cell surface receptor involved in signaling.RTK…
Q: The dominant motif found in hemoglobin and myoglobin is: a) twisted beta sheet b) beta barrel c)…
A: 1)The dominant motif found in hemoglobin and myoglobin is (b) beta barrel.2)myoglobin consists of (d…
Q: An enzyme with concentration of 3.0 uM is added to a flask containing 125 uM of substrate. You know…
A: The observed velocity (Vo) in enzyme kinetics denotes the initial rate of an enzyme-catalyzed…
Q: Determine whether each of the carbohydrates is a monosaccharide, disaccharide, or polysaccharide.
A: Carbohydrates are biomolecules that comprise carbon, hydrogen and oxygen atoms. The hydrogen-oxygen…
Q: Complete the Haworth projection of B-D-fructose. The anomeric carbon is shown. C H Answer Bank OH…
A:
Q: If a cell were given glucose labeled at carbon-3 with 14C, what carbon(s) of pyruvate would contain…
A: Glycolysis is a process by which a cell converts one glucose molecule to 2 molecules of pyruvate.…
Q: Choose the TRUE statement. A phospholipid micelle (not a liposome) can be characterized as Forming…
A: Phospholipids are important components of cell membranes and can form various structures in aqueous…
Q: ANSWER THE FOLLOWING PROBLEM AND EXPLAIN YOUR ANSWER FOR BETTER UNDERSTANDING. 7.) Which Two Amino…
A: There are four classes of biological macromolecules: they are proteins, nucleic acids, lipids and…
Q: What is the difference between fast and slow glycoly
A: Fast and slow glycolysis are two distinct metabolic pathways that cells employ to generate ATP…
Q: What is the process of beta oxidation
A: Beta-oxidation is a metabolic process that occurs in the mitochondria of cells and is responsible…
Q: You identify a section of 2 beta strands in a protein crystal structure. When you identify the…
A: The linear arrangement of amino acids in beta strands, an usual secondary structure in proteins,…
Q: Match the descriptions to the type of G protein. Heterotrimeric G proteins monomeric in both active…
A: G proteins (Guanosine nucleotide-binding proteins) can be broadly classified into two types. They…
Q: Why are several glycine residues found within the selectivity filter of K+ channels? a. Glycine…
A: The selectivity filter of potassium channels is the structural element directly responsible for the…
Q: TPCK is a molecule with large hydrophobic groups. Why does TPCK inactivate chymotrypsin but not…
A: Proteases are enzymes responsible for cleaving peptide bonds. Chymotrypsin and trypsin are both…
Q: Draw the chemical mechanism (with electron-pushing arrows) for the addition of an RNA nucleotide…
A: The process by which m RNA is synthesized from DNA is known as transcription. RNA polymerases are…
Q: 10) Explain how the Km and [VO] of a reaction between a fixed amount of catalase and a gradually…
A: An allosteric inhibitor doesn't competes with the substrate for binding at active site. It binds to…
Q: N Signal Sequence The schematic above depicts a protein with the indicated signaling sequences along…
A: Protein sorting is the process where the proteins are transported to their particular destination or…
Q: The scientific name of the fructose molecule shown below is CH2OH OH НО ОН CH₂OH
A: Fructofuranose is a structure which is similar to the furan ring, which has a five carbon membered…
Q: An enzyme catalyzes a reaction with a Km of 6.50 mM and a V of 4.45 mMs. Calculate the reaction…
A: The Michaelis-Menten equation to calculate the reaction velocity of enzymatic catalytic reaction is…
Q: Biktarvy, shown below, is a new drug that is being investigated for the treatment of HIV. но OH F…
A: In biochemistry a functional group is a group of atoms that is responsible for the chemical…
Q: Glycogen can form alpha 1-->4 and beta 1--> 6 linkages alpha 1--> 4 and alpha 1--> 6 linkages alpha…
A: Classification of carbohydrates: Monosaccharide: it composed of single sugar molecule. Example:…
Q: A) Effect of Enzymes on Reactions Which of the following effects (a-g) would increase the rate of an…
A: We know Keq is the equilibrium constant and it is equal to .Here;[P] is the concentration of product…
Q: A protein has binding affinity for its ligand of KA = 2 x 105 1/M at pH 5.0 and room temperature. a.…
A: Kd is the dissociation constant for a protein-ligand complex that measures the affinity with which a…
Q: Dimethoate is a commonly used agrochemical. However, chronic exposure to it can cause an individual…
A: Hyper-ß-alaninemia is a condition characterized by excessive alanine in the muscles. In the context…
Q: Imagine you need to move a large protein from one region in the cell to another. Which of the…
A: Nucleases are enzymes that break down nucleic acids (DNA and RNA) by cleaving the phosphodiester…
Q: Identify factors that account for the high phosphoryl-transfer potential of the nucleoside…
A: High phosphoryl transfer potential is the measure of tendency of a molecule to donate its phosphate…
Q: The most immediate result of ATP hydrolysis for the sodium/potassium ATPase is the... binding of ADP…
A: Na,K-ATPase is a membrane protein which plays a vital role in the cell. It pumps Na+ and K+ ions…
Q: In one type of hemoglobin variant, lysine EF6 is replaced by an aspartic acid residue. What would be…
A: The replacement of lysine (K) by aspartic acid (D) at position EF6 in hemoglobin affects the…
Q: You discover a new polysaccharide that has no branching whatsoever. What do you predict is the role…
A: A polysaccharide is a type of carbohydrate made up of long chains of sugar molecules linked…
Q: Assume a fragment of two-stranded antiparallel β-structure is 3.3 nm long. What is the approximate…
A: Deoxyribonucleic acid also known as DNA, is a molecule that houses the genetic instructions required…
Step by step
Solved in 4 steps
- In your acid phosphatase enzyme kinetics lab, you constructed a Lineweaver-Burk plot. Lefs assume that you graphicaly obtain 1/Vmax - 9.33 (umoliey and -1Km--0.012 M. Which of the following is correct? A) Vmax = 9.33 (no units) B) Km = 0.012 (no units) C) Vmax = (1/Vru" = 0.107 umolis D) Km =-14-1K = 83.3 pM E) Both C) and D) are correctPlease note the following Lineweaver-Burk plot for the enzyme Virbraniumase reacting with a substrate: -0.4 . 1/V 5 4 3 2 -0.2 0 0.2 y = 5.2781x + 1.3338 R² = 0.9967 0.4 0.6 0.8 1/[S] 1 Based on the information provided, what is the Vmax for this reaction?An enzyme has the following values: vmax's; 0.028 M s-' and 0.021 M s-' (I), and km's; 0.00198 M and 0.00197 M (I), when the total concentration of enzyme is 10-º M. The (I) values were determined with 1 microM inhibitor. Select the value closest to the k, for this enzyme. (hint 1 microM = 10-6 M).
- From a kinetics experiment, the Vmax was determined to be 450µM∙min-1. For the kinetic assay, 0.1mL of a 0.05mg/mL solution of enzyme was used, and the enzyme has a molecular weight of 125,000 g/mole. Assume a reaction volume of 700µL. Calculate the kcat (in sec-1) for the enzyme.The enzyme serine hydroxymethy ltransferase (SHMT) catalyses the conversion of serine into glycine. The fo llowing table gives the initial rates, vo, for the SHMT-catalysed reaction of the substrate serine at var ious concentratio ns of serine, lSI.[S]/(mmol dm-3) 10 20 30 40vo(μmol dm-3 s-1) 1.63 2.94 4.10 4.95Use the data to determine the values of the MichaelisMenten constant, the maximum velocity of the reaction, and the maximum turnover number of the enzyme.A Lineweaver-Burk plot generates a line with the following formula: y = 0.3x + 0.4. What is the Km of this enzyme?
- Suppose the biochemical pathway represented in Equation 5-10a is coupled at its first step to the hydrolysis of ATP to ADP. How would this change the expres- sion for AG of the pathway?If a data from an enzyme experiment is plotted as a Lineweaver - Burk plot, and the Vmax is 0.02 sec/mol, and x-intercept is -2.5 mM-1, then what is the KM value?From a kinetics experiment, kcat was determined to be 295sec-1. For the kinetic assay, 0.3mL of a 0.25mg/mL solution of enzyme was used, and the enzyme has a molecular weight of 125,000 g/mole. Assume a reaction volume of 3mL. Calculate Vmax (µM∙min-1) for the enzyme and catalytic efficiency ( in M-1sec-1) for the enzyme. The Km for the enzyme was determined to be 2.55 x 10-2M.
- Use the concept of Hess' Law to determine AH for the process V 2X +Z given the following numbered processes: V 2 W AH1 W X + Y AH2 Z- 2Y AH3 ΔΗ+ ΔH +ΔΗ3 ΟΔΗ+ 2ΔΗ-ΔΗ3 ΟΔΗ+ 2ΔΗ2+ ΔΗ3 ΔΗ + ΔH,-ΔΗ3What is the likely keq for an enzyme that has a deltaG =-18.3kj/mol?In a pUC19 digest for 1 ug of pUC19 (DNA conc. 282ng/ul) using 10X Cutsmart buffer, pure water and BamHI enzyme in a total volume of 40 ul, how much of each solution is added to the total volume?