Filtration does/is NOT...? a) Sterilize heat sensitive liquids b) Kill microbes c) Sterilize providing pores are exceedingly small d) Used in preparation of vaccines, vitamins, and drugs e) Used to capture microbes in ventilation systems via HEPA filters Which of the following is false? a) Pasteurization does not sterilize b) Ionization radiation is useful for sterilizing food and pharmaceuticals c) Phenolics can be used as antiseptics d) Isopropyl alcohol can sterilize the skin e) Steam autoclaving is an effective means of sterilization Which of the following is not a factor that determines the effectiveness of an antimicrobial agent? a)  Time of exposure b)  Temperature c)  pH d)  Concentration of agent e)  All of the above are factors that determines the effectiveness of an antimicrobial agent 4. Which of the following is not a specific cell target for antimicrobial agents? a) Capsule b) Cell wall c) Cell membrane d) DNA / RNA synthesis e) Protein function 5. Which of the following antimicrobial drugs target cell wall synthesis? a) Ciprofloxacin b) Sulfa drugs c) Gentamycin d) Penicillin e) Polymyxin B 6. Which of the following antimicrobial drugs target DNA/RNA synthesis? a) Ciprofloxacin b) Sulfa drugs c) Gentamycin d) Penicillin e) Polymyxin B 7. Which of the following antimicrobial drugs target protein synthesis? a) Ciprofloxacin b) Sulfa drugs c) Gentamycin d) Penicillin e) Polymyxin B 8. Which of the following antimicrobial drugs target specific metabolic pathways? a) Ciprofloxacin b) Sulfa drugs c) Gentamycin d) Penicillin e) Polymyxin B   9.Which of the following antimicrobial drugs target the cell membrane? a) Ciprofloxacin b) Sulfa drugs c) Gentamycin d) Penicillin e) Polymyxin B 10. The process by which two cells arise from one is known as a) conjugation. b) binary fission. c) meiosis. d) mitosis. 11. The time interval required for the formation of two cells from one is called the a) generation time. b) growth time. c) growth rate. d) division rate 12. What temperature is most commonly used for autoclaves to sterilize growth media and other devices prior to experimentation? a) 95°C b) 101°C c) 121°C d) 140°C 13. A chemical that denatures proteins is MOST likely to be classified as a(n) ________ agent. a) antiseptic b) bacteriostatic c) bacteriocidal d) detergent True or false for the following questions:  The presence of Escherichia coli in water is used as an indication of fecal contamination. The Vibrio species are characterized as being curved, gram negative bacteria with polar flagella. Chlamydia, Rickettsia and Mycoplasma are all obligate intracellular parasites. Neisseria, Staphylococcus, and Streptococcus are all gram-positive cocci. Tetanus, botulism, and diphtheria are diseases caused by gram positive, soil-dwelling, spore- forming rods. Most togaviruses are transmitted to humans via insect bites. The reason why dogs do not get measles is because their cells lack the correct receptor sites for that virus. Glycoprotein spikes are found on the capsids of viruses. A naked virus lacks a capsid. The polioviruses and hepatitis A virus are transmitted by contaminated food or water. Staphylococcus grows best in a high salt environment. Streptococcus is killed by growing on blood agar. True or False of the following questions:  Antibiotics can be used to treat viral infections. Gram positive bacteria stain pink; Gram negative bacteria stain purple when using the Gram- staining technique. Gram staining color results are based on differences in the bacterial cell wall composition. Peptidoglycan is a glycoprotein found in all forms of life. Penicillin only works in bacterial cells that are undergoing mitosis. Vaccines cause autism and other severe developmental difficulties. Effective hand washing (in warm water, with soap, and for a time frame of 2 minutes) can prevent most common infections. Match the following physical methods of sterilization with their most common uses:  a. Pasteurization b.Incineration c. Ionizing radiation d. Non-ionizing radiation e. Filtration 1. Commonly used to sterilize water, vaccines, blood products, and air. 2. Commonly used to decontaminate milk and fruit juices. 3. Commonly used to treat meat, spices and tissues for grafts. 4. Commonly used to sterilize loops and needles in lab. 5. Commonly used to purify air in hospitals. Match the following drug targets with the type of antibiotic that uses them (answers may be used more than once or not at all). a. Cell wall b. Cell membrane c. Protein synthesis (Transcription/Translation) d. Metabolic Pathway e. Nucleic Acid synthesis Chloramphenicol Clindamycin Erythromycin Doxycycline Sulfonamides Matching: Match the following terms to the appropriate organisms.  a. gram negative cocci b. gram positive rods c.gram positive cocci d.gram negative rods e. gram negative spirals Campylobacter Staphylococcus Neisseria Klebsiella Corynebacterium  Match the following: a. Corynebacteria b. Mycobacteria c. Streptomycetes d. Mycoplasmas e. Enterics filamentous, resemble fungi acid-fast lack cell walls can be termed coliforms club shaped

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  1. Filtration does/is NOT...?
    a) Sterilize heat sensitive liquids
    b) Kill microbes
    c) Sterilize providing pores are exceedingly small
    d) Used in preparation of vaccines, vitamins, and drugs
    e) Used to capture microbes in ventilation systems via HEPA filters

  2. Which of the following is false?
    a) Pasteurization does not sterilize
    b) Ionization radiation is useful for sterilizing food and pharmaceuticals c) Phenolics can be used as antiseptics
    d) Isopropyl alcohol can sterilize the skin
    e) Steam autoclaving is an effective means of sterilization

    1. Which of the following is not a factor that determines the effectiveness of an antimicrobial agent?

      a)  Time of exposure
    2. b)  Temperature
    3. c)  pH
    4. d)  Concentration of agent
    5. e)  All of the above are factors that determines the effectiveness of an antimicrobial agent

      4. Which of the following is not a specific cell target for antimicrobial agents? a) Capsule

      b) Cell wall
      c) Cell membrane
      d) DNA / RNA synthesis e) Protein function

      5. Which of the following antimicrobial drugs target cell wall synthesis? a) Ciprofloxacin

      b) Sulfa drugs c) Gentamycin d) Penicillin

    e) Polymyxin B

    6. Which of the following antimicrobial drugs target DNA/RNA synthesis? a) Ciprofloxacin

    b) Sulfa drugs c) Gentamycin d) Penicillin
    e) Polymyxin B

    7. Which of the following antimicrobial drugs target protein synthesis? a) Ciprofloxacin

    b) Sulfa drugs c) Gentamycin d) Penicillin
    e) Polymyxin B

    8. Which of the following antimicrobial drugs target specific metabolic pathways? a) Ciprofloxacin

    b) Sulfa drugs c) Gentamycin d) Penicillin
    e) Polymyxin B

 

9.Which of the following antimicrobial drugs target the cell membrane? a) Ciprofloxacin

b) Sulfa drugs c) Gentamycin d) Penicillin
e) Polymyxin B

10. The process by which two cells arise from one is known as a) conjugation.

b) binary fission. c) meiosis.
d) mitosis.

11. The time interval required for the formation of two cells from one is called the a) generation time.

b) growth time. c) growth rate. d) division rate

12. What temperature is most commonly used for autoclaves to sterilize growth media and other devices prior to experimentation?

a) 95°C b) 101°C c) 121°C d) 140°C

13. A chemical that denatures proteins is MOST likely to be classified as a(n) ________ agent. a) antiseptic

b) bacteriostatic c) bacteriocidal d) detergent

True or false for the following questions: 

  1. The presence of Escherichia coli in water is used as an indication of fecal contamination.

  2. The Vibrio species are characterized as being curved, gram negative bacteria with polar flagella.

  3. Chlamydia, Rickettsia and Mycoplasma are all obligate intracellular parasites.

  4. Neisseria, Staphylococcus, and Streptococcus are all gram-positive cocci.

  5. Tetanus, botulism, and diphtheria are diseases caused by gram positive, soil-dwelling, spore-

    forming rods.

  6. Most togaviruses are transmitted to humans via insect bites.

  7. The reason why dogs do not get measles is because their cells lack the correct receptor sites for

    that virus.

  8. Glycoprotein spikes are found on the capsids of viruses.

  9. A naked virus lacks a capsid.

  10. The polioviruses and hepatitis A virus are transmitted by contaminated food or water.

  11. Staphylococcus grows best in a high salt environment.

  12. Streptococcus is killed by growing on blood agar.

True or False of the following questions: 

  1. Antibiotics can be used to treat viral infections.

  2. Gram positive bacteria stain pink; Gram negative bacteria stain purple when using the Gram-

    staining technique.

  3. Gram staining color results are based on differences in the bacterial cell wall composition.

  4. Peptidoglycan is a glycoprotein found in all forms of life.

  5. Penicillin only works in bacterial cells that are undergoing mitosis.

  6. Vaccines cause autism and other severe developmental difficulties.

  7. Effective hand washing (in warm water, with soap, and for a time frame of 2 minutes) can prevent most common infections.

Match the following physical methods of sterilization with their most common uses: 

a. Pasteurization

b.Incineration

c. Ionizing radiation

d. Non-ionizing radiation

e. Filtration

1. Commonly used to sterilize water, vaccines, blood products, and air.

2. Commonly used to decontaminate milk and fruit juices.
3. Commonly used to treat meat, spices and tissues for grafts.

4. Commonly used to sterilize loops and needles in lab.

5. Commonly used to purify air in hospitals.

Match the following drug targets with the type of antibiotic that uses them (answers may be used more than once or not at all).

a. Cell wall

b. Cell membrane

c. Protein synthesis (Transcription/Translation)

d. Metabolic Pathway

e. Nucleic Acid synthesis

  1. Chloramphenicol

  2. Clindamycin

  3. Erythromycin

  4. Doxycycline

  5. Sulfonamides

Matching: Match the following terms to the appropriate organisms. 

a. gram negative cocci

b. gram positive rods

c.gram positive cocci

d.gram negative rods

e. gram negative spirals

  1. Campylobacter

  2. Staphylococcus

  3. Neisseria

  4. Klebsiella

  5. Corynebacterium

 Match the following:

a. Corynebacteria

b. Mycobacteria

c. Streptomycetes

d. Mycoplasmas

e. Enterics

  1. filamentous, resemble fungi

  2. acid-fast

  3. lack cell walls

  4. can be termed coliforms

  5. club shaped

  6.  
 
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