Explain how you would seperate Taq polymerase(PDB: 1TAQ) using one or series of chromatography methods from a cell extract.
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Q: Can two-dimensional gel electrophoresis be used as a purificationtechnique? Explain.
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Q: describe how lysozyme may or may not be separated by thin-layer chromatography
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Explain how you would seperate Taq polymerase(PDB: 1TAQ) using one or series of chromatography methods from a cell extract.
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- Would you expect transfer of chromosomal DNA by conjugation to be more efficient if cells were plated together on solid medium (agar) or mixed together in a liquid in a shaking flask? Explain.Give three reasons why liposomes/nanoparticles are attractive drug delivery system for recombinant protein preparations?Suppose you have a cell-lysis sample containing genomic DNA and a complex mixture of proteins and debris. Describe different commonly used analytical methods to i) separate and purify DNA contents from other cellular contents. ii) amplify a gene of interest if present in the genomic DNA.
- What is the purpose of using Triton X in hemolytic assay.describe each step carefully and use your own words. Steps will be like following; I) Primer design for SLIC cloning and the protocol II) Transformation and sequence validation of the clones III) Culture of HEK293T IV) Transduction of HEK293T cells with plasmid V) Western Blotting VI) Immunfluorescence for Confocal MicroscopyWhich of the DNAs shown in Figure would move fastest during agarose gel electrophoresis?
- It is desired to isolate genomic DNA from liquid culture of S. cerevisiae yeast. A commercial kit will be used to isolate genomic DNA from this liquid culture. Answer the following questions to understand the strategy used by commercial kits for genomic DNA isolation. a) List all the steps from cell pellet preparation to DNA elution. b) With which feature can the membrane in the column that comes with the commercial kit bind DNA? c) Which component in the kit would you use to recover the DNA from the membrane of the column to which the DNA was attached?Since higher concentration colcemid will result in shorter chromosome, you want to change your protocol and reduce final concentration of colcemid in your 10 ml blood culture from 0.1ug/ml to 0.05ug/ml. how many ul colcemid stock solution with concentration10ug/ml needed to be added in 10ml blood culture?What other method could be used to purify lysozyme from white using its unusual pl?
- What are the different categories of cell viability assays? Describe one of them briefly. Define the role (aim of use) of Trypan blue dye in cell culture studies.Give advantages and disadvantages of using commercially available kits for plasmid extraction over conventional alkaline lysis technique.Fluorescence in-situ hybridisation (FISH) analysis can be performed on fixed pathological tumour sections. Briefly outline why interphase FISH is used on fixed material from a solid tumour, rather than metaphase FISH