Draw the Western Blot that would result from undertaking the same experiment in the presence of: i) An estrogen receptor agonist ii) An estrogen receptor antagonist iii) An estrogen receptor agonist in the presence of an excess amount of antagonist iv) Estrogen in the presence of an excess amount of antagonist v) An estrogen receptor inverse agonist.
Draw the Western Blot that would result from undertaking the same experiment in the presence of: i) An estrogen receptor agonist ii) An estrogen receptor antagonist iii) An estrogen receptor agonist in the presence of an excess amount of antagonist iv) Estrogen in the presence of an excess amount of antagonist v) An estrogen receptor inverse agonist.
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Question
Draw the Western Blot that would result from undertaking the same experiment in the presence of:
i) An estrogen receptor agonist
ii) An estrogen receptor antagonist
iii) An estrogen receptor agonist in the presence of an excess amount of antagonist
iv) Estrogen in the presence of an excess amount of antagonist
v) An estrogen receptor inverse agonist.
Transcribed Image Text:This question relates to the biological activity of the estrogen receptor (ER). ER is a
nuclear receptor and positively regulates the expression the c-Myc gene, a
transcription factor found at elevated levels in numerous cancers.
To understand the effect of drugs on the biological behaviour of the estrogen receptor
in cells, you undertake Western Blotting analysis of a cell line that expresses a
recombinant human estrogen receptor. After culturing the cells, you then lyse the cells
and fractionate them, generating membrane, cytosolic and nuclear protein fractions
from the whole cell lysate. After running these fractions on SDS-PAGE gels, you
transfer to nitrocellulose membrane and probe with monoclonal antibody conjugates
for the proteins ER and c-Myc.
The results of your Western Blotting experiment without any treatment are provided in
Figure 1.
Primary antibody
Cell fraction
Anti-ER
Anti-c-myc
Membrane
Cytoplasm
Nucleus
| |
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